Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics
Abstract Dermal papilla cells (DPCs) play roles in key functions of the epidermis such as hair generation. The use of human induced pluripotent cells (hiPSCs) makes it possible to obtain DP-like cells and study the molecular mechanisms of DPC development during embryogenesis. In this work, we studie...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-40398-w |
| _version_ | 1797453080912986112 |
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| author | Andrei Riabinin Ekaterina Kalabusheva Anastasia Khrustaleva Mikhail Akulinin Alexander Tyakht Egor Osidak Elina Chermnykh Andrey Vasiliev Ekaterina Vorotelyak |
| author_facet | Andrei Riabinin Ekaterina Kalabusheva Anastasia Khrustaleva Mikhail Akulinin Alexander Tyakht Egor Osidak Elina Chermnykh Andrey Vasiliev Ekaterina Vorotelyak |
| author_sort | Andrei Riabinin |
| collection | DOAJ |
| description | Abstract Dermal papilla cells (DPCs) play roles in key functions of the epidermis such as hair generation. The use of human induced pluripotent cells (hiPSCs) makes it possible to obtain DP-like cells and study the molecular mechanisms of DPC development during embryogenesis. In this work, we studied the phenotypic trajectory of hiPSCs during their differentiation into DP-like cells and evaluated the epithelial-mesenchymal interaction potential of the resulting cell line. Specifically, we differentiated hiPSCs into neural progenitor cells (NPCs) and subsequently into DP-like cells. Analysis of bulk RNA-seq data during this process enabled us to observe gene expression dynamics during five stages of dermal differentiation. Furthermore, functional assays (organoids in both collagen gels and hanging drop cultures and tubulogenesis assays) revealed that the dermal cell lines we generated could interact with epidermal cells. |
| first_indexed | 2024-03-09T15:17:47Z |
| format | Article |
| id | doaj.art-ac0f4e342a8646819d2c017624669d02 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:17:47Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ac0f4e342a8646819d2c017624669d022023-11-26T13:00:59ZengNature PortfolioScientific Reports2045-23222023-08-0113111210.1038/s41598-023-40398-wTrajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristicsAndrei Riabinin0Ekaterina Kalabusheva1Anastasia Khrustaleva2Mikhail Akulinin3Alexander Tyakht4Egor Osidak5Elina Chermnykh6Andrey Vasiliev7Ekaterina Vorotelyak8Koltzov Institute of Developmental Biology of Russian Academy of SciencesKoltzov Institute of Developmental Biology of Russian Academy of SciencesInstitute of Gene Biology of Russian Academy of SciencesInstitute of Gene Biology of Russian Academy of SciencesInstitute of Gene Biology of Russian Academy of SciencesImtek LTDKoltzov Institute of Developmental Biology of Russian Academy of SciencesKoltzov Institute of Developmental Biology of Russian Academy of SciencesKoltzov Institute of Developmental Biology of Russian Academy of SciencesAbstract Dermal papilla cells (DPCs) play roles in key functions of the epidermis such as hair generation. The use of human induced pluripotent cells (hiPSCs) makes it possible to obtain DP-like cells and study the molecular mechanisms of DPC development during embryogenesis. In this work, we studied the phenotypic trajectory of hiPSCs during their differentiation into DP-like cells and evaluated the epithelial-mesenchymal interaction potential of the resulting cell line. Specifically, we differentiated hiPSCs into neural progenitor cells (NPCs) and subsequently into DP-like cells. Analysis of bulk RNA-seq data during this process enabled us to observe gene expression dynamics during five stages of dermal differentiation. Furthermore, functional assays (organoids in both collagen gels and hanging drop cultures and tubulogenesis assays) revealed that the dermal cell lines we generated could interact with epidermal cells.https://doi.org/10.1038/s41598-023-40398-w |
| spellingShingle | Andrei Riabinin Ekaterina Kalabusheva Anastasia Khrustaleva Mikhail Akulinin Alexander Tyakht Egor Osidak Elina Chermnykh Andrey Vasiliev Ekaterina Vorotelyak Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics Scientific Reports |
| title | Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics |
| title_full | Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics |
| title_fullStr | Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics |
| title_full_unstemmed | Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics |
| title_short | Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics |
| title_sort | trajectory of hipscs derived neural progenitor cells differentiation into dermal papilla like cells and their characteristics |
| url | https://doi.org/10.1038/s41598-023-40398-w |
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