Modulation of Active Gut Microbiota by Lactobacillus rhamnosus GG in a Diet Induced Obesity Murine Model
Gut microbiota play a key role in the development of metabolic disorders. Defining and correlating structural shifts in gut microbial assemblages with conditions related to metabolic syndrome have, however, been proven difficult. Results from 16S genomic DNA and 16S ribosomal RNA analyses of fecal s...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00710/full |
| _version_ | 1828764463597092864 |
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| author | Yosep Ji Soyoung Park Haryung Park Eunchong Hwang Hyeunkil Shin Bruno Pot Wilhelm H. Holzapfel |
| author_facet | Yosep Ji Soyoung Park Haryung Park Eunchong Hwang Hyeunkil Shin Bruno Pot Wilhelm H. Holzapfel |
| author_sort | Yosep Ji |
| collection | DOAJ |
| description | Gut microbiota play a key role in the development of metabolic disorders. Defining and correlating structural shifts in gut microbial assemblages with conditions related to metabolic syndrome have, however, been proven difficult. Results from 16S genomic DNA and 16S ribosomal RNA analyses of fecal samples may differ widely, leading to controversial information on the whole microbial community and metabolically active microbiota. Using a C57BL/6J murine model, we compared data from 16S genomic DNA and ribosomal RNA of the fecal microbiota. The study included three groups of experimental animals comprising two groups with high fat diet induced obesity (DIO) while a third group (control) received a low fat diet. One of the DIO groups was treated with the probiotic Lactobacillus rhamnosus GG (LGG). Compared to the data obtained by DNA analysis, a significantly higher abundance of OTUs was accounted for by RNA analysis. Moreover, rRNA based analysis showed a modulation of the active gut microbial population in the DIO group receiving LGG, thus reflecting a change in the induced obesity status of the host. As one of the most widely studied probiotics the functionality of LGG has been linked to the alleviation of metabolic syndrome, and, in some cases, to an impact on the microbiome. Yet, it appears that no study has reported thus far on modulation of the active microbiota by LGG treatment. It is postulated that the resulting impact on calorie consumption affects weight gain concomitantly with modulation of the functional structure of the gut microbial population. Using the 16S rRNA based approach therefore decisively increased the precision of gut microbiota metagenome analysis. |
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| id | doaj.art-ac1036a7106c439d94d181de4a06d38c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T02:22:23Z |
| publishDate | 2018-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ac1036a7106c439d94d181de4a06d38c2022-12-22T01:24:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-04-01910.3389/fmicb.2018.00710344249Modulation of Active Gut Microbiota by Lactobacillus rhamnosus GG in a Diet Induced Obesity Murine ModelYosep Ji0Soyoung Park1Haryung Park2Eunchong Hwang3Hyeunkil Shin4Bruno Pot5Wilhelm H. Holzapfel6Graduate School of Advanced Green Energy and Environment, Handong Global University, Pohang, South KoreaGraduate School of Advanced Green Energy and Environment, Handong Global University, Pohang, South KoreaGraduate School of Advanced Green Energy and Environment, Handong Global University, Pohang, South KoreaGraduate School of Advanced Green Energy and Environment, Handong Global University, Pohang, South KoreaSchool of Life Sciences, Handong Global University, Pohang, South KoreaResearch Group of Industrial Microbiology and Food Biotechnology, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Brussels, BelgiumGraduate School of Advanced Green Energy and Environment, Handong Global University, Pohang, South KoreaGut microbiota play a key role in the development of metabolic disorders. Defining and correlating structural shifts in gut microbial assemblages with conditions related to metabolic syndrome have, however, been proven difficult. Results from 16S genomic DNA and 16S ribosomal RNA analyses of fecal samples may differ widely, leading to controversial information on the whole microbial community and metabolically active microbiota. Using a C57BL/6J murine model, we compared data from 16S genomic DNA and ribosomal RNA of the fecal microbiota. The study included three groups of experimental animals comprising two groups with high fat diet induced obesity (DIO) while a third group (control) received a low fat diet. One of the DIO groups was treated with the probiotic Lactobacillus rhamnosus GG (LGG). Compared to the data obtained by DNA analysis, a significantly higher abundance of OTUs was accounted for by RNA analysis. Moreover, rRNA based analysis showed a modulation of the active gut microbial population in the DIO group receiving LGG, thus reflecting a change in the induced obesity status of the host. As one of the most widely studied probiotics the functionality of LGG has been linked to the alleviation of metabolic syndrome, and, in some cases, to an impact on the microbiome. Yet, it appears that no study has reported thus far on modulation of the active microbiota by LGG treatment. It is postulated that the resulting impact on calorie consumption affects weight gain concomitantly with modulation of the functional structure of the gut microbial population. Using the 16S rRNA based approach therefore decisively increased the precision of gut microbiota metagenome analysis.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00710/full16S rDNA analysis16S rRNA analysisgut microbiotaobesityLGG |
| spellingShingle | Yosep Ji Soyoung Park Haryung Park Eunchong Hwang Hyeunkil Shin Bruno Pot Wilhelm H. Holzapfel Modulation of Active Gut Microbiota by Lactobacillus rhamnosus GG in a Diet Induced Obesity Murine Model Frontiers in Microbiology 16S rDNA analysis 16S rRNA analysis gut microbiota obesity LGG |
| title | Modulation of Active Gut Microbiota by Lactobacillus rhamnosus GG in a Diet Induced Obesity Murine Model |
| title_full | Modulation of Active Gut Microbiota by Lactobacillus rhamnosus GG in a Diet Induced Obesity Murine Model |
| title_fullStr | Modulation of Active Gut Microbiota by Lactobacillus rhamnosus GG in a Diet Induced Obesity Murine Model |
| title_full_unstemmed | Modulation of Active Gut Microbiota by Lactobacillus rhamnosus GG in a Diet Induced Obesity Murine Model |
| title_short | Modulation of Active Gut Microbiota by Lactobacillus rhamnosus GG in a Diet Induced Obesity Murine Model |
| title_sort | modulation of active gut microbiota by lactobacillus rhamnosus gg in a diet induced obesity murine model |
| topic | 16S rDNA analysis 16S rRNA analysis gut microbiota obesity LGG |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00710/full |
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