Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients
Abstract Background It has been demonstrated previously that induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (MSCs) have immunosuppressive effects on activated T cells. However, the effects of iPSC-MSCs on quiescent T cells are still unknown. The aim of this study was to identify...
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | http://link.springer.com/article/10.1186/s13287-018-0896-z |
| _version_ | 1818968185826705408 |
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| author | Xing-Liang Fan Qing-Xiang Zeng Xin Li Cheng-Lin Li Zhi-Bin Xu Xue-Quan Deng Jianbo Shi Dong Chen Song Guo Zheng Qing-Ling Fu |
| author_facet | Xing-Liang Fan Qing-Xiang Zeng Xin Li Cheng-Lin Li Zhi-Bin Xu Xue-Quan Deng Jianbo Shi Dong Chen Song Guo Zheng Qing-Ling Fu |
| author_sort | Xing-Liang Fan |
| collection | DOAJ |
| description | Abstract Background It has been demonstrated previously that induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (MSCs) have immunosuppressive effects on activated T cells. However, the effects of iPSC-MSCs on quiescent T cells are still unknown. The aim of this study was to identify the immunomodulatory role of iPSC-MSCs on resting peripheral blood mononuclear cells (PBMCs) from allergic rhinitis (AR) patients. Methods PBMCs were cocultured with iPSC-MSCs without any stimulation, following which lymphocyte proliferation, activation of T cells, TH1/TH2 and regulatory T (Treg) cell differentiation, and Treg cell function were analyzed. The roles of soluble factors and cell–cell contact were examined to investigate the mechanisms involved. Results iPSC-MSCs promoted the proliferation of resting lymphocytes, activated CD4+ and CD8+ T cells, and upregulated and activated Treg cells without any additional stimulation. In addition, iPSC-MSCs balanced biased TH1/TH2 cytokine levels. Cell–cell contact was confirmed to be a possible mechanism involved. NF-κB was identified to play an important role in the immunomodulatory effects of iPSC-MSCs on quiescent T cells. Conclusions iPSC-MSCs activate quiescent T cells and elevate regulatory T-cell response in AR patients, suggesting different immunomodulatory functions of iPSC-MSCs according to the phases of diseases. Therefore, iPSC-MSCs are a potential therapeutic candidate for treating allergic airway inflammation. |
| first_indexed | 2024-12-20T14:00:41Z |
| format | Article |
| id | doaj.art-ac104de859d24c9882f8cc3575cab7a0 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-20T14:00:41Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-ac104de859d24c9882f8cc3575cab7a02022-12-21T19:38:23ZengBMCStem Cell Research & Therapy1757-65122018-06-019111510.1186/s13287-018-0896-zInduced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patientsXing-Liang Fan0Qing-Xiang Zeng1Xin Li2Cheng-Lin Li3Zhi-Bin Xu4Xue-Quan Deng5Jianbo Shi6Dong Chen7Song Guo Zheng8Qing-Ling Fu9Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen UniversityOtorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen UniversityDepartment of Emergency, Guangdong General Hospital, Guangdong Academy of Medical ScienceOtorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen UniversityOtorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen UniversityOtorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen UniversityOtorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen UniversityOtorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen UniversityDepartment of Clinical Immunology, The Third Affiliated Hospital, Sun Yat-sen UniversityOtorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen UniversityAbstract Background It has been demonstrated previously that induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (MSCs) have immunosuppressive effects on activated T cells. However, the effects of iPSC-MSCs on quiescent T cells are still unknown. The aim of this study was to identify the immunomodulatory role of iPSC-MSCs on resting peripheral blood mononuclear cells (PBMCs) from allergic rhinitis (AR) patients. Methods PBMCs were cocultured with iPSC-MSCs without any stimulation, following which lymphocyte proliferation, activation of T cells, TH1/TH2 and regulatory T (Treg) cell differentiation, and Treg cell function were analyzed. The roles of soluble factors and cell–cell contact were examined to investigate the mechanisms involved. Results iPSC-MSCs promoted the proliferation of resting lymphocytes, activated CD4+ and CD8+ T cells, and upregulated and activated Treg cells without any additional stimulation. In addition, iPSC-MSCs balanced biased TH1/TH2 cytokine levels. Cell–cell contact was confirmed to be a possible mechanism involved. NF-κB was identified to play an important role in the immunomodulatory effects of iPSC-MSCs on quiescent T cells. Conclusions iPSC-MSCs activate quiescent T cells and elevate regulatory T-cell response in AR patients, suggesting different immunomodulatory functions of iPSC-MSCs according to the phases of diseases. Therefore, iPSC-MSCs are a potential therapeutic candidate for treating allergic airway inflammation.http://link.springer.com/article/10.1186/s13287-018-0896-zInduced pluripotent stem cell-derived mesenchymal stem cellsAllergic rhinitisQuiescent T cellsImmunomodulationNF-κB |
| spellingShingle | Xing-Liang Fan Qing-Xiang Zeng Xin Li Cheng-Lin Li Zhi-Bin Xu Xue-Quan Deng Jianbo Shi Dong Chen Song Guo Zheng Qing-Ling Fu Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients Stem Cell Research & Therapy Induced pluripotent stem cell-derived mesenchymal stem cells Allergic rhinitis Quiescent T cells Immunomodulation NF-κB |
| title | Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients |
| title_full | Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients |
| title_fullStr | Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients |
| title_full_unstemmed | Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients |
| title_short | Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients |
| title_sort | induced pluripotent stem cell derived mesenchymal stem cells activate quiescent t cells and elevate regulatory t cell response via nf κb in allergic rhinitis patients |
| topic | Induced pluripotent stem cell-derived mesenchymal stem cells Allergic rhinitis Quiescent T cells Immunomodulation NF-κB |
| url | http://link.springer.com/article/10.1186/s13287-018-0896-z |
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