Determination of Optimum Conditions for Ergosterol Production by Saccharomyces cerevisiae
Ergosterol is an important provitamin in the present-day of industrial biotechnology. Seven yeast strains were obtained from the market of Basrah city and subjected to screening for their ergosterol production ability using liquid-state fermentation, the cultural conditions, and nutritional requirem...
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| Format: | Article |
| Language: | English |
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University of Basrah
2022-08-01
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| Series: | Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ |
| Subjects: | |
| Online Access: | https://www.bajas.edu.iq/BJAS/index.php/bjas/article/view/656 |
| _version_ | 1818009628046262272 |
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| author | Esraa S. Ethafa Alaa J. A. Al-Manhel |
| author_facet | Esraa S. Ethafa Alaa J. A. Al-Manhel |
| author_sort | Esraa S. Ethafa |
| collection | DOAJ |
| description | Ergosterol is an important provitamin in the present-day of industrial biotechnology. Seven yeast strains were obtained from the market of Basrah city and subjected to screening for their ergosterol production ability using liquid-state fermentation, the cultural conditions, and nutritional requirements for optimal production of ergosterol by Saccharomyces cerevisiae under laboratory conditions were determined. Y.6 is the best isolate of the yeast that produced ergosterol. It was identified as S. cerevisiae, with a similarity rate of 97% by using the Vitek2 device, this S. cerevisiae (Y.6) was further subjected to optimization conditions. The results showed that the best medium for production was yeast extract peptone dextrose broth. The effect of two cheaper carbon sources, molasses and date juice were investigated. Maximum ergosterol (0.55% ) was produced using a medium containing date juice, with a replacement ratio of 75%, an incubation time of 72 hours, pH 5, at a temperature of 30 °C, and an inoculation volume of 4 ml. The percentage of ergosterol was (0.47, 0.55, 0.74, 0.66, 0.68 and 0.78 %), respectively.
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| first_indexed | 2024-04-14T05:44:09Z |
| format | Article |
| id | doaj.art-ac10ca418a6048d1a99248e9ad0d58b4 |
| institution | Directory Open Access Journal |
| issn | 1814-5868 2520-0860 |
| language | English |
| last_indexed | 2024-04-14T05:44:09Z |
| publishDate | 2022-08-01 |
| publisher | University of Basrah |
| record_format | Article |
| series | Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ |
| spelling | doaj.art-ac10ca418a6048d1a99248e9ad0d58b42022-12-22T02:09:20ZengUniversity of BasrahMaǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ1814-58682520-08602022-08-0135210.37077/25200860.2022.35.2.03Determination of Optimum Conditions for Ergosterol Production by Saccharomyces cerevisiaeEsraa S. Ethafa0Alaa J. A. Al-Manhel1Department of Food Science, College of Agriculture, University of Basrah, IraqDepartment of Food Science, College of Agriculture, University of Basrah, IraqErgosterol is an important provitamin in the present-day of industrial biotechnology. Seven yeast strains were obtained from the market of Basrah city and subjected to screening for their ergosterol production ability using liquid-state fermentation, the cultural conditions, and nutritional requirements for optimal production of ergosterol by Saccharomyces cerevisiae under laboratory conditions were determined. Y.6 is the best isolate of the yeast that produced ergosterol. It was identified as S. cerevisiae, with a similarity rate of 97% by using the Vitek2 device, this S. cerevisiae (Y.6) was further subjected to optimization conditions. The results showed that the best medium for production was yeast extract peptone dextrose broth. The effect of two cheaper carbon sources, molasses and date juice were investigated. Maximum ergosterol (0.55% ) was produced using a medium containing date juice, with a replacement ratio of 75%, an incubation time of 72 hours, pH 5, at a temperature of 30 °C, and an inoculation volume of 4 ml. The percentage of ergosterol was (0.47, 0.55, 0.74, 0.66, 0.68 and 0.78 %), respectively. https://www.bajas.edu.iq/BJAS/index.php/bjas/article/view/656Ergosterol productionOptimum conditionsSaccharomyces cerevisiaeScreening |
| spellingShingle | Esraa S. Ethafa Alaa J. A. Al-Manhel Determination of Optimum Conditions for Ergosterol Production by Saccharomyces cerevisiae Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ Ergosterol production Optimum conditions Saccharomyces cerevisiae Screening |
| title | Determination of Optimum Conditions for Ergosterol Production by Saccharomyces cerevisiae |
| title_full | Determination of Optimum Conditions for Ergosterol Production by Saccharomyces cerevisiae |
| title_fullStr | Determination of Optimum Conditions for Ergosterol Production by Saccharomyces cerevisiae |
| title_full_unstemmed | Determination of Optimum Conditions for Ergosterol Production by Saccharomyces cerevisiae |
| title_short | Determination of Optimum Conditions for Ergosterol Production by Saccharomyces cerevisiae |
| title_sort | determination of optimum conditions for ergosterol production by saccharomyces cerevisiae |
| topic | Ergosterol production Optimum conditions Saccharomyces cerevisiae Screening |
| url | https://www.bajas.edu.iq/BJAS/index.php/bjas/article/view/656 |
| work_keys_str_mv | AT esraasethafa determinationofoptimumconditionsforergosterolproductionbysaccharomycescerevisiae AT alaajaalmanhel determinationofoptimumconditionsforergosterolproductionbysaccharomycescerevisiae |