Investigation of the expression of P53 gene in bystander cells after therapeutic ultrasound exposure
Background: Bystander effect is defined as biological responses observed in cells that are not exposed to radiation directly but these radiation effects are induced through messages from irradiated cells. One of the non-invasive methods for cancer treatment is ultrasound waves. The therapeutic effic...
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Kashan University of Medical Sciences and Health Services
2019-10-01
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| Online Access: | http://feyz.kaums.ac.ir/article-1-3785-en.html |
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| author | Rezaei M Kamran-Samani R Shanei A Kazemi M Hejazi SH |
| author_facet | Rezaei M Kamran-Samani R Shanei A Kazemi M Hejazi SH |
| author_sort | Rezaei M |
| collection | DOAJ |
| description | Background: Bystander effect is defined as biological responses observed in cells that are not exposed to radiation directly but these radiation effects are induced through messages from irradiated cells. One of the non-invasive methods for cancer treatment is ultrasound waves. The therapeutic efficacy of ultrasound waveslike treatment with ionizing radiation can be altered by the bystander effect, so it is necessary to evaluate the bystander effect after ultrasound exposure. In this study, the effect of ultrasound exposure on the expression of P53 gene in neighboring cells has been investigated.
Materials and Methods: The cells were divided into three groups: control (without treatment), target (under direct ultrasound exposure) and the bystander (no radiation, only receiving target cells culture media). In the target group, the cells were exposed to the ultrasound and after one hour their culture medium were transferred to the bystander group. Cell viability and the expression of P53 gene by using the MTT assay and Real-time PCR was analyzed.
Results: The percentage of cell survival in the target group was reduced compared to the control group, but in the bystander group, this difference was not significant. The expression of P53 gene in the bystander group was statistically increased compared to the control group.
Conclusion: The bystander effect is induced after ultrasound exposure and it can increase the level of P53 gene expression in bystander cells. |
| first_indexed | 2024-12-18T19:21:40Z |
| format | Article |
| id | doaj.art-ac111539c1d141d186b1b0b26b55e260 |
| institution | Directory Open Access Journal |
| issn | 1029-7855 2008-9821 |
| language | fas |
| last_indexed | 2024-12-18T19:21:40Z |
| publishDate | 2019-10-01 |
| publisher | Kashan University of Medical Sciences and Health Services |
| record_format | Article |
| series | Fiyz̤ |
| spelling | doaj.art-ac111539c1d141d186b1b0b26b55e2602022-12-21T20:55:59ZfasKashan University of Medical Sciences and Health ServicesFiyz̤1029-78552008-98212019-10-01234344351Investigation of the expression of P53 gene in bystander cells after therapeutic ultrasound exposureRezaei M0Kamran-Samani R1Shanei A2Kazemi M3Hejazi SH4Department of Medical Physics, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. IranDepartment of Medical Physics, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. IranDepartment of Medical Physics, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. IranDepartment of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.Department of Parasitology and Mycology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.Background: Bystander effect is defined as biological responses observed in cells that are not exposed to radiation directly but these radiation effects are induced through messages from irradiated cells. One of the non-invasive methods for cancer treatment is ultrasound waves. The therapeutic efficacy of ultrasound waveslike treatment with ionizing radiation can be altered by the bystander effect, so it is necessary to evaluate the bystander effect after ultrasound exposure. In this study, the effect of ultrasound exposure on the expression of P53 gene in neighboring cells has been investigated. Materials and Methods: The cells were divided into three groups: control (without treatment), target (under direct ultrasound exposure) and the bystander (no radiation, only receiving target cells culture media). In the target group, the cells were exposed to the ultrasound and after one hour their culture medium were transferred to the bystander group. Cell viability and the expression of P53 gene by using the MTT assay and Real-time PCR was analyzed. Results: The percentage of cell survival in the target group was reduced compared to the control group, but in the bystander group, this difference was not significant. The expression of P53 gene in the bystander group was statistically increased compared to the control group. Conclusion: The bystander effect is induced after ultrasound exposure and it can increase the level of P53 gene expression in bystander cells.http://feyz.kaums.ac.ir/article-1-3785-en.htmlUltrasound wavesBystander effectMTT assayReal-time PCRP53 gene |
| spellingShingle | Rezaei M Kamran-Samani R Shanei A Kazemi M Hejazi SH Investigation of the expression of P53 gene in bystander cells after therapeutic ultrasound exposure Fiyz̤ Ultrasound waves Bystander effect MTT assay Real-time PCR P53 gene |
| title | Investigation of the expression of P53 gene in bystander cells after therapeutic ultrasound exposure |
| title_full | Investigation of the expression of P53 gene in bystander cells after therapeutic ultrasound exposure |
| title_fullStr | Investigation of the expression of P53 gene in bystander cells after therapeutic ultrasound exposure |
| title_full_unstemmed | Investigation of the expression of P53 gene in bystander cells after therapeutic ultrasound exposure |
| title_short | Investigation of the expression of P53 gene in bystander cells after therapeutic ultrasound exposure |
| title_sort | investigation of the expression of p53 gene in bystander cells after therapeutic ultrasound exposure |
| topic | Ultrasound waves Bystander effect MTT assay Real-time PCR P53 gene |
| url | http://feyz.kaums.ac.ir/article-1-3785-en.html |
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