In vivo biodistribution, antioxidant and hemolysis tendency of superparamagnetic iron oxide nanoparticles – Potential anticancer agents
Ferromagnetic and superparamagnetic oxide nanoparticles are of particular attention because of their possible use in various fields ranging from bio-nanotechnology to spintronics. Detailed magnetic, dielectric and impedance investigations are crucial for the above-mentioned applications. This study...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223000631 |
| _version_ | 1811159982654095360 |
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| author | Hera N Khan M Imran Ifra Sanaullah Irfan Ullah Khan Anjum N Sabri Shahzad Naseem Saira Riaz |
| author_facet | Hera N Khan M Imran Ifra Sanaullah Irfan Ullah Khan Anjum N Sabri Shahzad Naseem Saira Riaz |
| author_sort | Hera N Khan |
| collection | DOAJ |
| description | Ferromagnetic and superparamagnetic oxide nanoparticles are of particular attention because of their possible use in various fields ranging from bio-nanotechnology to spintronics. Detailed magnetic, dielectric and impedance investigations are crucial for the above-mentioned applications. This study deals with the exploration of various iron oxide phases under as-synthesized conditions by sol–gel method. pH of the sols is varied in the range of 1 to 11. X-ray diffraction (XRD) analysis indicate amorphous behavior for nanoparticles synthesized using pH 1 and 3. Nanoparticles synthesized using pH 2 and 4–6 exhibit hematite phase of iron oxide. Whereas structural transition to maghemite phase is observed for pH 7–8. Nanoparticles synthesized using high pH values, i.e. 9–11, exhibit structural transition towards magnetite phase of iron oxide. Hematite nanoparticles exhibit superparamagnetic and ferromagnetic hysteresis curves with saturation magnetization of ∼ 24 emu/g and ∼ 13–17 emu/g at pH 2 and pH 4–6, respectively. Maghemite nanoparticles exhibit superparamagnetic (pH 7) and ferromagnetic (pH 8) response with saturation magnetization of ∼ 69 and ∼ 42 emu/g, respectively. Fe3O4 nanoparticles exhibit superparamagnetic (pH 9–10) and ferromagnetic (pH 11) behavior with saturation magnetization of ∼ 88, 87 and 52 emu/g, respectively. High grain boundary resistance contributed towards high dielectric constant of ∼ 99, 109 and 154 (log f = 5.0) at pH 2, 7 and 9. Detailed impedance values indicate dominant role of grain boundaries in the conductivity of iron oxide nanoparticles. Superparamagnetic iron oxide (pH 9) exhibits strong antioxidant activity along with a very weak hemolytic response. The findings of cell lysis reveal that synthesized nanoparticles have a potential to combat dangerous cancer cells. Drug efficacy results show that after 120 min the encapsulation efficacy reaches a peak of ∼ 83 % using curcumin, a naturally existing drug. In vivo biodistribution of nanoparticles was studied in Rabbit model. Synthesized nanoparticles are labelled using Technetium-99 m. Whereas, labeling efficacy and stability was examined using =nstant thin layer chromatography (ITLC) process. In vitro and in vivo results suggest potential anti-cancer applications of as-synthesized superparamagnetic nanoparticles. |
| first_indexed | 2024-04-10T05:49:57Z |
| format | Article |
| id | doaj.art-ac12eb4dce69422289ad481c8366f823 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-10T05:49:57Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-ac12eb4dce69422289ad481c8366f8232023-03-05T04:23:59ZengElsevierArabian Journal of Chemistry1878-53522023-04-01164104602In vivo biodistribution, antioxidant and hemolysis tendency of superparamagnetic iron oxide nanoparticles – Potential anticancer agentsHera N Khan0M Imran1Ifra Sanaullah2Irfan Ullah Khan3Anjum N Sabri4Shahzad Naseem5Saira Riaz6Centre of Excellence in Solid State Physics, University of the Punjab, Lahore 54590, Pakistan; Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore 54590, PakistanCentre of Excellence in Solid State Physics, University of the Punjab, Lahore 54590, PakistanCentre of Excellence in Solid State Physics, University of the Punjab, Lahore 54590, PakistanDepartment of Cyclotron and Allied Radiopharmaceutics, INMOL Cancer Hospital, New Campus Road, Lahore 54600, PakistanDepartment of Microbiology and Molecular Genetics, University of the Punjab, Lahore 54590, PakistanCentre of Excellence in Solid State Physics, University of the Punjab, Lahore 54590, Pakistan; Corresponding authors.Centre of Excellence in Solid State Physics, University of the Punjab, Lahore 54590, Pakistan; Corresponding authors.Ferromagnetic and superparamagnetic oxide nanoparticles are of particular attention because of their possible use in various fields ranging from bio-nanotechnology to spintronics. Detailed magnetic, dielectric and impedance investigations are crucial for the above-mentioned applications. This study deals with the exploration of various iron oxide phases under as-synthesized conditions by sol–gel method. pH of the sols is varied in the range of 1 to 11. X-ray diffraction (XRD) analysis indicate amorphous behavior for nanoparticles synthesized using pH 1 and 3. Nanoparticles synthesized using pH 2 and 4–6 exhibit hematite phase of iron oxide. Whereas structural transition to maghemite phase is observed for pH 7–8. Nanoparticles synthesized using high pH values, i.e. 9–11, exhibit structural transition towards magnetite phase of iron oxide. Hematite nanoparticles exhibit superparamagnetic and ferromagnetic hysteresis curves with saturation magnetization of ∼ 24 emu/g and ∼ 13–17 emu/g at pH 2 and pH 4–6, respectively. Maghemite nanoparticles exhibit superparamagnetic (pH 7) and ferromagnetic (pH 8) response with saturation magnetization of ∼ 69 and ∼ 42 emu/g, respectively. Fe3O4 nanoparticles exhibit superparamagnetic (pH 9–10) and ferromagnetic (pH 11) behavior with saturation magnetization of ∼ 88, 87 and 52 emu/g, respectively. High grain boundary resistance contributed towards high dielectric constant of ∼ 99, 109 and 154 (log f = 5.0) at pH 2, 7 and 9. Detailed impedance values indicate dominant role of grain boundaries in the conductivity of iron oxide nanoparticles. Superparamagnetic iron oxide (pH 9) exhibits strong antioxidant activity along with a very weak hemolytic response. The findings of cell lysis reveal that synthesized nanoparticles have a potential to combat dangerous cancer cells. Drug efficacy results show that after 120 min the encapsulation efficacy reaches a peak of ∼ 83 % using curcumin, a naturally existing drug. In vivo biodistribution of nanoparticles was studied in Rabbit model. Synthesized nanoparticles are labelled using Technetium-99 m. Whereas, labeling efficacy and stability was examined using =nstant thin layer chromatography (ITLC) process. In vitro and in vivo results suggest potential anti-cancer applications of as-synthesized superparamagnetic nanoparticles.http://www.sciencedirect.com/science/article/pii/S1878535223000631Sol–gelIron oxidepHMagneticAntioxidantHemolysis |
| spellingShingle | Hera N Khan M Imran Ifra Sanaullah Irfan Ullah Khan Anjum N Sabri Shahzad Naseem Saira Riaz In vivo biodistribution, antioxidant and hemolysis tendency of superparamagnetic iron oxide nanoparticles – Potential anticancer agents Arabian Journal of Chemistry Sol–gel Iron oxide pH Magnetic Antioxidant Hemolysis |
| title | In vivo biodistribution, antioxidant and hemolysis tendency of superparamagnetic iron oxide nanoparticles – Potential anticancer agents |
| title_full | In vivo biodistribution, antioxidant and hemolysis tendency of superparamagnetic iron oxide nanoparticles – Potential anticancer agents |
| title_fullStr | In vivo biodistribution, antioxidant and hemolysis tendency of superparamagnetic iron oxide nanoparticles – Potential anticancer agents |
| title_full_unstemmed | In vivo biodistribution, antioxidant and hemolysis tendency of superparamagnetic iron oxide nanoparticles – Potential anticancer agents |
| title_short | In vivo biodistribution, antioxidant and hemolysis tendency of superparamagnetic iron oxide nanoparticles – Potential anticancer agents |
| title_sort | in vivo biodistribution antioxidant and hemolysis tendency of superparamagnetic iron oxide nanoparticles potential anticancer agents |
| topic | Sol–gel Iron oxide pH Magnetic Antioxidant Hemolysis |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223000631 |
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