Analysis of Serum Metabolomics in Obese Mice Induced by High-Fat Diet
Li Bao,1,2 Chunjing Yang,1,2 Zhengyuan Shi,1,2 Zhanrong Wang,3 Dechun Jiang1,2 1Department of Pharmacy, Beijing Shijitan Hospital, Capital Medical University, Beijing, People’s Republic of China; 2Beijing Key Laboratory of Biocharacteristic Profiling for Evaluation of Rational Drug Use, Beijing, Peo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-11-01
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| Series: | Diabetes, Metabolic Syndrome and Obesity |
| Subjects: | |
| Online Access: | https://www.dovepress.com/analysis-of-serum-metabolomics-in-obese-mice-induced-by-high-fat-diet-peer-reviewed-fulltext-article-DMSO |
| _version_ | 1811174234327613440 |
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| author | Bao L Yang C Shi Z Wang Z Jiang D |
| author_facet | Bao L Yang C Shi Z Wang Z Jiang D |
| author_sort | Bao L |
| collection | DOAJ |
| description | Li Bao,1,2 Chunjing Yang,1,2 Zhengyuan Shi,1,2 Zhanrong Wang,3 Dechun Jiang1,2 1Department of Pharmacy, Beijing Shijitan Hospital, Capital Medical University, Beijing, People’s Republic of China; 2Beijing Key Laboratory of Biocharacteristic Profiling for Evaluation of Rational Drug Use, Beijing, People’s Republic of China; 3Department of Traditional Chinese Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, People’s Republic of ChinaCorrespondence: Zhanrong WangDepartment of Traditional Chinese Medicine, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Haidian District, Beijing 100038, People’s Republic of China, Tel +86-10-6392-6405Email dor_j@163.comDechun JiangDepartment of Pharmacy, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Haidian District, Beijing 100038, People’s Republic of China, Tel +86-10-6392-6723Email jiangdechun@sina.comBackground: Obesity is a public health problem all over the world, and dietary habits are considered one of the important reasons.Methods: In this study, serum metabolites of mice fed a normal or high-fat diet (HFD) were analyzed using UPLC-QTOF-MS.Results: A significant increase in body weight was noted in HFD mice. The HFD and control groups were significantly different from each other on OPLS-DA scores. The major metabolites contributing to obesity were lipid metabolites (phosphatidylcholines, phosphatidylethanolamine, and lysophosphatidylcholines). In addition, this study revealed that glycerophospholipid metabolism, α-linolenic acid metabolism, and linoleic acid metabolism were related to obesity and obesity-associated diseases.Conclusion: These results can be used to better understand obesity and assess its risk, which will provide new ideas for treatment.Keywords: obesity, serum metabolomics, high-fat diet, UPLC-QTOF-MS technique |
| first_indexed | 2024-04-10T18:00:49Z |
| format | Article |
| id | doaj.art-ac141a20301a40b4acd68b862d588572 |
| institution | Directory Open Access Journal |
| issn | 1178-7007 |
| language | English |
| last_indexed | 2024-04-10T18:00:49Z |
| publishDate | 2021-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Diabetes, Metabolic Syndrome and Obesity |
| spelling | doaj.art-ac141a20301a40b4acd68b862d5885722023-02-02T16:02:24ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072021-11-01Volume 144671467871023Analysis of Serum Metabolomics in Obese Mice Induced by High-Fat DietBao LYang CShi ZWang ZJiang DLi Bao,1,2 Chunjing Yang,1,2 Zhengyuan Shi,1,2 Zhanrong Wang,3 Dechun Jiang1,2 1Department of Pharmacy, Beijing Shijitan Hospital, Capital Medical University, Beijing, People’s Republic of China; 2Beijing Key Laboratory of Biocharacteristic Profiling for Evaluation of Rational Drug Use, Beijing, People’s Republic of China; 3Department of Traditional Chinese Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, People’s Republic of ChinaCorrespondence: Zhanrong WangDepartment of Traditional Chinese Medicine, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Haidian District, Beijing 100038, People’s Republic of China, Tel +86-10-6392-6405Email dor_j@163.comDechun JiangDepartment of Pharmacy, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Haidian District, Beijing 100038, People’s Republic of China, Tel +86-10-6392-6723Email jiangdechun@sina.comBackground: Obesity is a public health problem all over the world, and dietary habits are considered one of the important reasons.Methods: In this study, serum metabolites of mice fed a normal or high-fat diet (HFD) were analyzed using UPLC-QTOF-MS.Results: A significant increase in body weight was noted in HFD mice. The HFD and control groups were significantly different from each other on OPLS-DA scores. The major metabolites contributing to obesity were lipid metabolites (phosphatidylcholines, phosphatidylethanolamine, and lysophosphatidylcholines). In addition, this study revealed that glycerophospholipid metabolism, α-linolenic acid metabolism, and linoleic acid metabolism were related to obesity and obesity-associated diseases.Conclusion: These results can be used to better understand obesity and assess its risk, which will provide new ideas for treatment.Keywords: obesity, serum metabolomics, high-fat diet, UPLC-QTOF-MS techniquehttps://www.dovepress.com/analysis-of-serum-metabolomics-in-obese-mice-induced-by-high-fat-diet-peer-reviewed-fulltext-article-DMSOobesityserum metabolomicshigh fat dietuplc-qtof-ms technique |
| spellingShingle | Bao L Yang C Shi Z Wang Z Jiang D Analysis of Serum Metabolomics in Obese Mice Induced by High-Fat Diet Diabetes, Metabolic Syndrome and Obesity obesity serum metabolomics high fat diet uplc-qtof-ms technique |
| title | Analysis of Serum Metabolomics in Obese Mice Induced by High-Fat Diet |
| title_full | Analysis of Serum Metabolomics in Obese Mice Induced by High-Fat Diet |
| title_fullStr | Analysis of Serum Metabolomics in Obese Mice Induced by High-Fat Diet |
| title_full_unstemmed | Analysis of Serum Metabolomics in Obese Mice Induced by High-Fat Diet |
| title_short | Analysis of Serum Metabolomics in Obese Mice Induced by High-Fat Diet |
| title_sort | analysis of serum metabolomics in obese mice induced by high fat diet |
| topic | obesity serum metabolomics high fat diet uplc-qtof-ms technique |
| url | https://www.dovepress.com/analysis-of-serum-metabolomics-in-obese-mice-induced-by-high-fat-diet-peer-reviewed-fulltext-article-DMSO |
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