Double Mutant Analysis with the Large Flower Mutant, <i>ohbana1</i>, to Explore the Regulatory Network Controlling the Flower and Seed Sizes in <i>Arabidopsis thaliana</i>
Two growth processes, cell proliferation and expansion, determine plant species-specific organ sizes. A large flower mutant in <i>Arabidopsis thaliana</i>, <i>ohbana1</i> (<i>ohb1</i>), was isolated from a mutant library. In the <i>ohb1</i> flowers, po...
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MDPI AG
2021-09-01
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| Series: | Plants |
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| author | Vuong Quoc Nhat Yusuke Kazama Kotaro Ishii Sumie Ohbu Hisato Kunitake Tomoko Abe Tomonari Hirano |
| author_facet | Vuong Quoc Nhat Yusuke Kazama Kotaro Ishii Sumie Ohbu Hisato Kunitake Tomoko Abe Tomonari Hirano |
| author_sort | Vuong Quoc Nhat |
| collection | DOAJ |
| description | Two growth processes, cell proliferation and expansion, determine plant species-specific organ sizes. A large flower mutant in <i>Arabidopsis thaliana</i>, <i>ohbana1</i> (<i>ohb1</i>), was isolated from a mutant library. In the <i>ohb1</i> flowers, post-mitotic cell expansion and endoreduplication of nuclear DNA were promoted. The whole-genome resequencing and genetic analysis results showed that the loss of function in <i>MEDIATOR16</i> (<i>MED16</i>), a mediator complex subunit, was responsible for the large flower phenotypes exhibited by <i>ohb1</i>. A phenotypic analysis of the mutant alleles in <i>MED16</i> and the double mutants created by crossing <i>ohb1</i> with representative large flower mutants revealed that MED16 and MED25 share part of the negative petal size regulatory pathways. Furthermore, the double mutant analyses suggested that there were genetically independent pathways leading to cell size restrictions in the floral organs which were not related to the MED complex. Several double mutants also formed larger and heavier seeds than the wild type and single mutant plants, which indicated that MED16 was involved in seed size regulation. This study has revealed part of the size-regulatory network in flowers and seeds through analysis of the <i>ohb1</i> mutant, and that the size-regulation pathways are partially different between floral organs and seeds. |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T07:17:37Z |
| publishDate | 2021-09-01 |
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| spelling | doaj.art-ac17f2df3c5f4739a60cf21cd323e7cb2023-11-22T14:53:09ZengMDPI AGPlants2223-77472021-09-01109188110.3390/plants10091881Double Mutant Analysis with the Large Flower Mutant, <i>ohbana1</i>, to Explore the Regulatory Network Controlling the Flower and Seed Sizes in <i>Arabidopsis thaliana</i>Vuong Quoc Nhat0Yusuke Kazama1Kotaro Ishii2Sumie Ohbu3Hisato Kunitake4Tomoko Abe5Tomonari Hirano6Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai Nishi, Miyazaki 889-2192, JapanFaculty of Bioscience and Biotechnology, Fukui Prefectural University, 4-1-1 Kenjojima, Matsuoka, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, JapanNishina Center for Accelerator-Based Science, RIKEN, 2-1 Hirosawa, Wako 351-0198, JapanNishina Center for Accelerator-Based Science, RIKEN, 2-1 Hirosawa, Wako 351-0198, JapanFaculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai Nishi, Miyazaki 889-2192, JapanNishina Center for Accelerator-Based Science, RIKEN, 2-1 Hirosawa, Wako 351-0198, JapanFaculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai Nishi, Miyazaki 889-2192, JapanTwo growth processes, cell proliferation and expansion, determine plant species-specific organ sizes. A large flower mutant in <i>Arabidopsis thaliana</i>, <i>ohbana1</i> (<i>ohb1</i>), was isolated from a mutant library. In the <i>ohb1</i> flowers, post-mitotic cell expansion and endoreduplication of nuclear DNA were promoted. The whole-genome resequencing and genetic analysis results showed that the loss of function in <i>MEDIATOR16</i> (<i>MED16</i>), a mediator complex subunit, was responsible for the large flower phenotypes exhibited by <i>ohb1</i>. A phenotypic analysis of the mutant alleles in <i>MED16</i> and the double mutants created by crossing <i>ohb1</i> with representative large flower mutants revealed that MED16 and MED25 share part of the negative petal size regulatory pathways. Furthermore, the double mutant analyses suggested that there were genetically independent pathways leading to cell size restrictions in the floral organs which were not related to the MED complex. Several double mutants also formed larger and heavier seeds than the wild type and single mutant plants, which indicated that MED16 was involved in seed size regulation. This study has revealed part of the size-regulatory network in flowers and seeds through analysis of the <i>ohb1</i> mutant, and that the size-regulation pathways are partially different between floral organs and seeds.https://www.mdpi.com/2223-7747/10/9/1881argon ion beamfloral organmutagenesisorgan sizepetalseed |
| spellingShingle | Vuong Quoc Nhat Yusuke Kazama Kotaro Ishii Sumie Ohbu Hisato Kunitake Tomoko Abe Tomonari Hirano Double Mutant Analysis with the Large Flower Mutant, <i>ohbana1</i>, to Explore the Regulatory Network Controlling the Flower and Seed Sizes in <i>Arabidopsis thaliana</i> Plants argon ion beam floral organ mutagenesis organ size petal seed |
| title | Double Mutant Analysis with the Large Flower Mutant, <i>ohbana1</i>, to Explore the Regulatory Network Controlling the Flower and Seed Sizes in <i>Arabidopsis thaliana</i> |
| title_full | Double Mutant Analysis with the Large Flower Mutant, <i>ohbana1</i>, to Explore the Regulatory Network Controlling the Flower and Seed Sizes in <i>Arabidopsis thaliana</i> |
| title_fullStr | Double Mutant Analysis with the Large Flower Mutant, <i>ohbana1</i>, to Explore the Regulatory Network Controlling the Flower and Seed Sizes in <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | Double Mutant Analysis with the Large Flower Mutant, <i>ohbana1</i>, to Explore the Regulatory Network Controlling the Flower and Seed Sizes in <i>Arabidopsis thaliana</i> |
| title_short | Double Mutant Analysis with the Large Flower Mutant, <i>ohbana1</i>, to Explore the Regulatory Network Controlling the Flower and Seed Sizes in <i>Arabidopsis thaliana</i> |
| title_sort | double mutant analysis with the large flower mutant i ohbana1 i to explore the regulatory network controlling the flower and seed sizes in i arabidopsis thaliana i |
| topic | argon ion beam floral organ mutagenesis organ size petal seed |
| url | https://www.mdpi.com/2223-7747/10/9/1881 |
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