Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci

Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci

Abstract Background The discovery of the CRISPR-Cas9 system and its applicability in mammalian embryos has revolutionized the way we generate genetically engineered animal models. To date, models harbouring conditional alleles (i.e. two loxP sites flanking an exon or a critical DNA sequence of inter...

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Bibliographic Details
Main Authors: Guillaume Bernas, Mariette Ouellet, Andréa Barrios, Hélène Jamann, Catherine Larochelle, Émile Lévy, Jean-François Schmouth
Format: Article
Language:English
Published: BMC 2022-05-01
Series:BMC Biotechnology
Subjects:
CRISPR
Electroporation
Conditional
loxP
Zygotes
Online Access:https://doi.org/10.1186/s12896-022-00744-8

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