Determination of Rebaudioside A in Mixed beer, Beer-based Beverages and Lemonades
The plant Stevia rebaudiana (Stevia sweet) is becoming a favourite sweetener in the food industry. Sweet taste is caused by diterpenic glycosides, so-called steviol glycosides, which lack calories while being 300 times sweeter than sucrose. While all the other glycosides are slightly or even disagre...
| Main Authors: | , |
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| Format: | Article |
| Language: | ces |
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Research Institute of Brewing and Malting, Plc.
2015-10-01
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| Series: | Kvasný průmysl |
| Subjects: | |
| Online Access: | http://www.kvasnyprumysl.eu/index.php/kp/article/view/157 |
| _version_ | 1818232927714017280 |
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| author | Marie Jurková Jana Olšovská |
| author_facet | Marie Jurková Jana Olšovská |
| author_sort | Marie Jurková |
| collection | DOAJ |
| description | The plant Stevia rebaudiana (Stevia sweet) is becoming a favourite sweetener in the food industry. Sweet taste is caused by diterpenic glycosides, so-called steviol glycosides, which lack calories while being 300 times sweeter than sucrose. While all the other glycosides are slightly or even disagreeably bitter, rebaudioside A possesses desirable sensorial properties. Because this sweetener is used to sweeten beverages including mixed beer, a fast routine analytical method was developed using HPLC with UV detection for determination of rebaudioside A in mixed beer and lemonade. The HILIC principle, which is suitable for separation of polar and hydrophilic analytes, was used for separation of steviol glycosides. The method is selective, reproducible and has a high recovery of 90–100 %. |
| first_indexed | 2024-12-12T11:14:04Z |
| format | Article |
| id | doaj.art-ac1a05f537d248968668caee7d5a58cd |
| institution | Directory Open Access Journal |
| issn | 2570-8619 |
| language | ces |
| last_indexed | 2024-12-12T11:14:04Z |
| publishDate | 2015-10-01 |
| publisher | Research Institute of Brewing and Malting, Plc. |
| record_format | Article |
| series | Kvasný průmysl |
| spelling | doaj.art-ac1a05f537d248968668caee7d5a58cd2022-12-22T00:26:13ZcesResearch Institute of Brewing and Malting, Plc.Kvasný průmysl2570-86192015-10-01619268–272268–27210.18832/kp2015025157Determination of Rebaudioside A in Mixed beer, Beer-based Beverages and LemonadesMarie Jurková0Jana Olšovská1Research Institute of Brewing and Malting, plc., Lípová 15, CZ 120 44 Praha 2Research Institute of Brewing and Malting, plc., Lípová 15, CZ 120 44 Praha 2The plant Stevia rebaudiana (Stevia sweet) is becoming a favourite sweetener in the food industry. Sweet taste is caused by diterpenic glycosides, so-called steviol glycosides, which lack calories while being 300 times sweeter than sucrose. While all the other glycosides are slightly or even disagreeably bitter, rebaudioside A possesses desirable sensorial properties. Because this sweetener is used to sweeten beverages including mixed beer, a fast routine analytical method was developed using HPLC with UV detection for determination of rebaudioside A in mixed beer and lemonade. The HILIC principle, which is suitable for separation of polar and hydrophilic analytes, was used for separation of steviol glycosides. The method is selective, reproducible and has a high recovery of 90–100 %.http://www.kvasnyprumysl.eu/index.php/kp/article/view/157SteviasweetenerbeerHPLC – UVHILIC |
| spellingShingle | Marie Jurková Jana Olšovská Determination of Rebaudioside A in Mixed beer, Beer-based Beverages and Lemonades Kvasný průmysl Stevia sweetener beer HPLC – UV HILIC |
| title | Determination of Rebaudioside A in Mixed beer, Beer-based Beverages and Lemonades |
| title_full | Determination of Rebaudioside A in Mixed beer, Beer-based Beverages and Lemonades |
| title_fullStr | Determination of Rebaudioside A in Mixed beer, Beer-based Beverages and Lemonades |
| title_full_unstemmed | Determination of Rebaudioside A in Mixed beer, Beer-based Beverages and Lemonades |
| title_short | Determination of Rebaudioside A in Mixed beer, Beer-based Beverages and Lemonades |
| title_sort | determination of rebaudioside a in mixed beer beer based beverages and lemonades |
| topic | Stevia sweetener beer HPLC – UV HILIC |
| url | http://www.kvasnyprumysl.eu/index.php/kp/article/view/157 |
| work_keys_str_mv | AT mariejurkova determinationofrebaudiosideainmixedbeerbeerbasedbeveragesandlemonades AT janaolsovska determinationofrebaudiosideainmixedbeerbeerbasedbeveragesandlemonades |