Developmental Expression of Calcium-Binding Protein-Containing Neurons in Neocortical Transplants
The present study examined the development of calcium binding protein-containing neurons in a timed series of fetal neocortical transplants. The immunoexpression of parvalbumin and calbindin, which are subpopulations of GABAergic neurons, have been widely studied in normal development and in disease...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
1998-03-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/096368979800700207 |
| _version_ | 1818228923522088960 |
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| author | Jeffrey M. Rosenstein Newton S. More Nina Mani Janette M. Krum |
| author_facet | Jeffrey M. Rosenstein Newton S. More Nina Mani Janette M. Krum |
| author_sort | Jeffrey M. Rosenstein |
| collection | DOAJ |
| description | The present study examined the development of calcium binding protein-containing neurons in a timed series of fetal neocortical transplants. The immunoexpression of parvalbumin and calbindin, which are subpopulations of GABAergic neurons, have been widely studied in normal development and in disease and injury states. Because of their purported resistance to oxidative injury by their ability to buffer Ca ++ influx, these neurons have been particularly studied following ischemia. Because it is likely that oxidative stress is associated with the grafting procedure, we sought to determine if these neurons displayed enhanced survival characteristics. Normally, parvalbumin and calbindin represent about 5-10% of cortical neurons. Within 2-4 wk after grafting the expression of both proteins increased markedly in that a relatively larger number of neurons (27% for parvalbumin) were immunopositive. This increase was transitory, however, and by 4 mo and beyond, confocal microscopic data showed a reduction of over 50% of parvalbumin (+) neurons and processes. Calbindin (+) processes showed a qualitative change in that they were smaller with less terminal branching. Electron microscopy confirmed a substantial reduction in parvalbumin synaptic contacts. Interestingly, in older grafts, remaining parvalbumin neurons were those that were strongly NSE (+) suggesting a link between normal metabolism and Ca ++ buffering in grafted neurons. It is possible that in early grafts certain neuronal populations transiently upregulated calcium binding proteins as a defensive mechanism against Ca ++ influx associated with oxidative stress. Over time, however, following physiological normalization within grafts, the calcium binding protein (+) neurons are diminished, possibly due to lack of appropriate afferent input to the interneuronal pool. |
| first_indexed | 2024-12-12T10:10:25Z |
| format | Article |
| id | doaj.art-ac1d51cf6aef46cca2a0262ae3a2701e |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-12T10:10:25Z |
| publishDate | 1998-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-ac1d51cf6aef46cca2a0262ae3a2701e2022-12-22T00:27:49ZengSAGE PublishingCell Transplantation0963-68971555-38921998-03-01710.1177/096368979800700207Developmental Expression of Calcium-Binding Protein-Containing Neurons in Neocortical TransplantsJeffrey M. Rosenstein0Newton S. More1Nina Mani2Janette M. Krum3Department of Anatomy and Cell Biology, The George Washington University Medical Center, Washington, DC 20027Department of Anatomy and Cell Biology, The George Washington University Medical Center, Washington, DC 20027Department of Anatomy and Cell Biology, The George Washington University Medical Center, Washington, DC 20027Department of Anatomy and Cell Biology, The George Washington University Medical Center, Washington, DC 20027The present study examined the development of calcium binding protein-containing neurons in a timed series of fetal neocortical transplants. The immunoexpression of parvalbumin and calbindin, which are subpopulations of GABAergic neurons, have been widely studied in normal development and in disease and injury states. Because of their purported resistance to oxidative injury by their ability to buffer Ca ++ influx, these neurons have been particularly studied following ischemia. Because it is likely that oxidative stress is associated with the grafting procedure, we sought to determine if these neurons displayed enhanced survival characteristics. Normally, parvalbumin and calbindin represent about 5-10% of cortical neurons. Within 2-4 wk after grafting the expression of both proteins increased markedly in that a relatively larger number of neurons (27% for parvalbumin) were immunopositive. This increase was transitory, however, and by 4 mo and beyond, confocal microscopic data showed a reduction of over 50% of parvalbumin (+) neurons and processes. Calbindin (+) processes showed a qualitative change in that they were smaller with less terminal branching. Electron microscopy confirmed a substantial reduction in parvalbumin synaptic contacts. Interestingly, in older grafts, remaining parvalbumin neurons were those that were strongly NSE (+) suggesting a link between normal metabolism and Ca ++ buffering in grafted neurons. It is possible that in early grafts certain neuronal populations transiently upregulated calcium binding proteins as a defensive mechanism against Ca ++ influx associated with oxidative stress. Over time, however, following physiological normalization within grafts, the calcium binding protein (+) neurons are diminished, possibly due to lack of appropriate afferent input to the interneuronal pool.https://doi.org/10.1177/096368979800700207 |
| spellingShingle | Jeffrey M. Rosenstein Newton S. More Nina Mani Janette M. Krum Developmental Expression of Calcium-Binding Protein-Containing Neurons in Neocortical Transplants Cell Transplantation |
| title | Developmental Expression of Calcium-Binding Protein-Containing Neurons in Neocortical Transplants |
| title_full | Developmental Expression of Calcium-Binding Protein-Containing Neurons in Neocortical Transplants |
| title_fullStr | Developmental Expression of Calcium-Binding Protein-Containing Neurons in Neocortical Transplants |
| title_full_unstemmed | Developmental Expression of Calcium-Binding Protein-Containing Neurons in Neocortical Transplants |
| title_short | Developmental Expression of Calcium-Binding Protein-Containing Neurons in Neocortical Transplants |
| title_sort | developmental expression of calcium binding protein containing neurons in neocortical transplants |
| url | https://doi.org/10.1177/096368979800700207 |
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