Anti-Cancer Activity of Catechin against A549 Lung Carcinoma Cells by Induction of Cyclin Kinase Inhibitor p21 and Suppression of Cyclin E1 and P–AKT
Catechin is one of the major polyphenols in teas, beans, and berry fruits. A number of studies have confirmed that catechins extract possesses health benefits in the prevention of various chronic diseases. In this study, the anti-cancer activity and mechanism of catechin against non-small cell lung...
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MDPI AG
2020-03-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/6/2065 |
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| author | Haiyan Sun Meichen Yin Danqing Hao Yixiao Shen |
| author_facet | Haiyan Sun Meichen Yin Danqing Hao Yixiao Shen |
| author_sort | Haiyan Sun |
| collection | DOAJ |
| description | Catechin is one of the major polyphenols in teas, beans, and berry fruits. A number of studies have confirmed that catechins extract possesses health benefits in the prevention of various chronic diseases. In this study, the anti-cancer activity and mechanism of catechin against non-small cell lung cancer A549 cells were investigated. The inhibitory rate of catechin on the proliferation of A549 cells reached 19.76% at a concentration of 600 μmol·L<sup>−1</sup> with 24 h incubation. The results demonstrated that catechin inhibits A549 cells by increasing the expressions of p21 and p27 in the cancer cells. Furthermore, the catechin treatment inhibited the expressions of cyclin E1 and phosphorylation of protein kinase (P−AKT) in a dose-dependent manner, which also contributed to the inhibition of cancer cell proliferation. Therefore, the results of this study indicated that catechin can effectively inhibit the proliferation of A549 cells through regulating its cell cycle arrest or indirectly via the p21 signaling pathway. It would provide important information for developing catechin and catechin-rich functional food or co-therapy for antitumor purposes. |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-13T19:24:25Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-ac1fb9065e17487886f6b6b24e595b212022-12-21T23:34:04ZengMDPI AGApplied Sciences2076-34172020-03-01106206510.3390/app10062065app10062065Anti-Cancer Activity of Catechin against A549 Lung Carcinoma Cells by Induction of Cyclin Kinase Inhibitor p21 and Suppression of Cyclin E1 and P–AKTHaiyan Sun0Meichen Yin1Danqing Hao2Yixiao Shen3Key Laboratory of Life Resources of Shaanxi Province, College of Biological Science and Engineering Shaanxi, University of Technology, Hanzhong 723000, ChinaKey Laboratory of Life Resources of Shaanxi Province, College of Biological Science and Engineering Shaanxi, University of Technology, Hanzhong 723000, ChinaKey Laboratory of Life Resources of Shaanxi Province, College of Biological Science and Engineering Shaanxi, University of Technology, Hanzhong 723000, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110161, ChinaCatechin is one of the major polyphenols in teas, beans, and berry fruits. A number of studies have confirmed that catechins extract possesses health benefits in the prevention of various chronic diseases. In this study, the anti-cancer activity and mechanism of catechin against non-small cell lung cancer A549 cells were investigated. The inhibitory rate of catechin on the proliferation of A549 cells reached 19.76% at a concentration of 600 μmol·L<sup>−1</sup> with 24 h incubation. The results demonstrated that catechin inhibits A549 cells by increasing the expressions of p21 and p27 in the cancer cells. Furthermore, the catechin treatment inhibited the expressions of cyclin E1 and phosphorylation of protein kinase (P−AKT) in a dose-dependent manner, which also contributed to the inhibition of cancer cell proliferation. Therefore, the results of this study indicated that catechin can effectively inhibit the proliferation of A549 cells through regulating its cell cycle arrest or indirectly via the p21 signaling pathway. It would provide important information for developing catechin and catechin-rich functional food or co-therapy for antitumor purposes.https://www.mdpi.com/2076-3417/10/6/2065catechinnon-small cell lung cancercyclineaktcyclin kinase inhibitor |
| spellingShingle | Haiyan Sun Meichen Yin Danqing Hao Yixiao Shen Anti-Cancer Activity of Catechin against A549 Lung Carcinoma Cells by Induction of Cyclin Kinase Inhibitor p21 and Suppression of Cyclin E1 and P–AKT Applied Sciences catechin non-small cell lung cancer cycline akt cyclin kinase inhibitor |
| title | Anti-Cancer Activity of Catechin against A549 Lung Carcinoma Cells by Induction of Cyclin Kinase Inhibitor p21 and Suppression of Cyclin E1 and P–AKT |
| title_full | Anti-Cancer Activity of Catechin against A549 Lung Carcinoma Cells by Induction of Cyclin Kinase Inhibitor p21 and Suppression of Cyclin E1 and P–AKT |
| title_fullStr | Anti-Cancer Activity of Catechin against A549 Lung Carcinoma Cells by Induction of Cyclin Kinase Inhibitor p21 and Suppression of Cyclin E1 and P–AKT |
| title_full_unstemmed | Anti-Cancer Activity of Catechin against A549 Lung Carcinoma Cells by Induction of Cyclin Kinase Inhibitor p21 and Suppression of Cyclin E1 and P–AKT |
| title_short | Anti-Cancer Activity of Catechin against A549 Lung Carcinoma Cells by Induction of Cyclin Kinase Inhibitor p21 and Suppression of Cyclin E1 and P–AKT |
| title_sort | anti cancer activity of catechin against a549 lung carcinoma cells by induction of cyclin kinase inhibitor p21 and suppression of cyclin e1 and p akt |
| topic | catechin non-small cell lung cancer cycline akt cyclin kinase inhibitor |
| url | https://www.mdpi.com/2076-3417/10/6/2065 |
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