Ultrasonic-assisted hydrothermal fabrication of selenium nanorods and their anticancer properties in prostate cancer cells and associated signaling pathway
This paper describes some useful information on the ultrasonic-assisted hydrothermal fabrication of selenium nanorods (abbreviated as SeNRs) and their potential anticancer activity. The synthesis of SeNRs was confirmed and their selective anticancer activity against DU-145 cells as a standard prosta...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535224001163 |
| _version_ | 1797214914170847232 |
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| author | Du Shen Shaosan Kang |
| author_facet | Du Shen Shaosan Kang |
| author_sort | Du Shen |
| collection | DOAJ |
| description | This paper describes some useful information on the ultrasonic-assisted hydrothermal fabrication of selenium nanorods (abbreviated as SeNRs) and their potential anticancer activity. The synthesis of SeNRs was confirmed and their selective anticancer activity against DU-145 cells as a standard prostate cancer cell line and human prostate normal cell line, RWPE-1 were assessed. The result of the FTIR study revealed the bending and stretching vibration of the Se-O bond. UV–vis spectroscopy data displayed a sharp maximum absorption (λmax) peak at 298 nm and the TEM image demonstrated that Se nanostructures had a rod-shaped morphology with lengths ranging from 50 to 100 nm and a diameter of around 10 nm. DLS data displayed that SeNRs had an average size of 204.68 nm and a zeta potential value of – 37.29 mV. Then, IC50 concentration of SeNRs against DU-145 and RWPE-1 cell lines were calculated to be about 15 µg/mL and 50.06 µg/mL, respectively, at 24 h. Also, it was deduced that SeNRs triggered their anticancer activity through the induction of membrane leakage, oxidative stress, apoptosis by overexpression of Bax/Bcl-2 ratio and caspase-9,-8, -3, and downexpression of AKT, PI3K, and mTOR mRNA and proteins, which was further verified by pre-incubation of cells with PI3K inhibitor, LY294002. Thus, the synthesized SeNRs can be used and developed as an effective nanostructure for the treatment of prostate cancer in future studies. |
| first_indexed | 2024-04-24T11:21:44Z |
| format | Article |
| id | doaj.art-ac201d1234d64386b73e0f65111696c5 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-24T11:21:44Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-ac201d1234d64386b73e0f65111696c52024-04-11T04:40:58ZengElsevierArabian Journal of Chemistry1878-53522024-05-01175105714Ultrasonic-assisted hydrothermal fabrication of selenium nanorods and their anticancer properties in prostate cancer cells and associated signaling pathwayDu Shen0Shaosan Kang1College of Clinic Medicine, North China University of Science and Technology, Tangshan 063000, ChinaDepartment of Urology, North China University of Technology Affiliated Hospital, Tangshan 063000, China; Corresponding author at: No 21. Bohai Avenue, Caofeidian, New Town, Tangshan 063000, China.This paper describes some useful information on the ultrasonic-assisted hydrothermal fabrication of selenium nanorods (abbreviated as SeNRs) and their potential anticancer activity. The synthesis of SeNRs was confirmed and their selective anticancer activity against DU-145 cells as a standard prostate cancer cell line and human prostate normal cell line, RWPE-1 were assessed. The result of the FTIR study revealed the bending and stretching vibration of the Se-O bond. UV–vis spectroscopy data displayed a sharp maximum absorption (λmax) peak at 298 nm and the TEM image demonstrated that Se nanostructures had a rod-shaped morphology with lengths ranging from 50 to 100 nm and a diameter of around 10 nm. DLS data displayed that SeNRs had an average size of 204.68 nm and a zeta potential value of – 37.29 mV. Then, IC50 concentration of SeNRs against DU-145 and RWPE-1 cell lines were calculated to be about 15 µg/mL and 50.06 µg/mL, respectively, at 24 h. Also, it was deduced that SeNRs triggered their anticancer activity through the induction of membrane leakage, oxidative stress, apoptosis by overexpression of Bax/Bcl-2 ratio and caspase-9,-8, -3, and downexpression of AKT, PI3K, and mTOR mRNA and proteins, which was further verified by pre-incubation of cells with PI3K inhibitor, LY294002. Thus, the synthesized SeNRs can be used and developed as an effective nanostructure for the treatment of prostate cancer in future studies.http://www.sciencedirect.com/science/article/pii/S1878535224001163SynthesisSelenium nanorodCharacterizationProstate cancerSignaling |
| spellingShingle | Du Shen Shaosan Kang Ultrasonic-assisted hydrothermal fabrication of selenium nanorods and their anticancer properties in prostate cancer cells and associated signaling pathway Arabian Journal of Chemistry Synthesis Selenium nanorod Characterization Prostate cancer Signaling |
| title | Ultrasonic-assisted hydrothermal fabrication of selenium nanorods and their anticancer properties in prostate cancer cells and associated signaling pathway |
| title_full | Ultrasonic-assisted hydrothermal fabrication of selenium nanorods and their anticancer properties in prostate cancer cells and associated signaling pathway |
| title_fullStr | Ultrasonic-assisted hydrothermal fabrication of selenium nanorods and their anticancer properties in prostate cancer cells and associated signaling pathway |
| title_full_unstemmed | Ultrasonic-assisted hydrothermal fabrication of selenium nanorods and their anticancer properties in prostate cancer cells and associated signaling pathway |
| title_short | Ultrasonic-assisted hydrothermal fabrication of selenium nanorods and their anticancer properties in prostate cancer cells and associated signaling pathway |
| title_sort | ultrasonic assisted hydrothermal fabrication of selenium nanorods and their anticancer properties in prostate cancer cells and associated signaling pathway |
| topic | Synthesis Selenium nanorod Characterization Prostate cancer Signaling |
| url | http://www.sciencedirect.com/science/article/pii/S1878535224001163 |
| work_keys_str_mv | AT dushen ultrasonicassistedhydrothermalfabricationofseleniumnanorodsandtheiranticancerpropertiesinprostatecancercellsandassociatedsignalingpathway AT shaosankang ultrasonicassistedhydrothermalfabricationofseleniumnanorodsandtheiranticancerpropertiesinprostatecancercellsandassociatedsignalingpathway |