Lack of in vivo genotoxic effect of dried whole Aloe ferox juice
Aloe ferox Mill is widely used as a traditional herbal medicine for the treatment of a broad spectrum of illnesses given its laxative, anti-inflammatory, bitter tonic, anti-oxidant, antimicrobial and anti-cancer properties.Using the in vivo alkaline comet assay in animals (OECD 489), this study inve...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Toxicology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750021001505 |
| _version_ | 1819179064816041984 |
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| author | Corrado L. Galli Serena Cinelli Paola Ciliutti Gloria Melzi Marina Marinovich |
| author_facet | Corrado L. Galli Serena Cinelli Paola Ciliutti Gloria Melzi Marina Marinovich |
| author_sort | Corrado L. Galli |
| collection | DOAJ |
| description | Aloe ferox Mill is widely used as a traditional herbal medicine for the treatment of a broad spectrum of illnesses given its laxative, anti-inflammatory, bitter tonic, anti-oxidant, antimicrobial and anti-cancer properties.Using the in vivo alkaline comet assay in animals (OECD 489), this study investigated the potential in vivo genotoxicity of dried Aloe ferox juice at dose levels of 500, 1000, and 2000 mg/kg/day in mice. Aloe ferox showed no genotoxic activity in preparations of single cells from the colon of the treated Hsd:ICR (CD-1) male mice. No statistically significant increase in DNA migration over the negative control was observed by analysis of variance for both comet parameters, tail moment and tail intensity, apart from the positive control ethyl methanesulphonate that induced clear and statistically significant increases in DNA migration parameters over the concurrent controls. The new reported scientific evidence unequivocally demonstrates that dried Aloe ferox juice containing hydroxyanthracene derivatives does not induce DNA damage in preparations of single cells from colon in in vivo comet genotoxicity studies. This suggests that the hyperplastic changes and mucosal hyperplasia observed after long-term administration of Aloe vera non-decolourised whole leaf extract may be attributed to an epigenetic effect of the material under investigation. |
| first_indexed | 2024-12-22T21:52:31Z |
| format | Article |
| id | doaj.art-ac22feb0e0ec4ce0a2aebeb0bf98780b |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-12-22T21:52:31Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-ac22feb0e0ec4ce0a2aebeb0bf98780b2022-12-21T18:11:20ZengElsevierToxicology Reports2214-75002021-01-01814711474Lack of in vivo genotoxic effect of dried whole Aloe ferox juiceCorrado L. Galli0Serena Cinelli1Paola Ciliutti2Gloria Melzi3Marina Marinovich4Department of Pharmacological and Biomolecular Sciences (DiSFeB) Section of Toxicology and Risk Assessment, University of Milan, Milan, Italy; Corresponding author.European Research Biology Center, ERBC, Pomezia, Rome, ItalyEuropean Research Biology Center, ERBC, Pomezia, Rome, ItalyDepartment of Pharmacological and Biomolecular Sciences (DiSFeB) Section of Toxicology and Risk Assessment, University of Milan, Milan, ItalyDepartment of Pharmacological and Biomolecular Sciences (DiSFeB) Section of Toxicology and Risk Assessment, University of Milan, Milan, ItalyAloe ferox Mill is widely used as a traditional herbal medicine for the treatment of a broad spectrum of illnesses given its laxative, anti-inflammatory, bitter tonic, anti-oxidant, antimicrobial and anti-cancer properties.Using the in vivo alkaline comet assay in animals (OECD 489), this study investigated the potential in vivo genotoxicity of dried Aloe ferox juice at dose levels of 500, 1000, and 2000 mg/kg/day in mice. Aloe ferox showed no genotoxic activity in preparations of single cells from the colon of the treated Hsd:ICR (CD-1) male mice. No statistically significant increase in DNA migration over the negative control was observed by analysis of variance for both comet parameters, tail moment and tail intensity, apart from the positive control ethyl methanesulphonate that induced clear and statistically significant increases in DNA migration parameters over the concurrent controls. The new reported scientific evidence unequivocally demonstrates that dried Aloe ferox juice containing hydroxyanthracene derivatives does not induce DNA damage in preparations of single cells from colon in in vivo comet genotoxicity studies. This suggests that the hyperplastic changes and mucosal hyperplasia observed after long-term administration of Aloe vera non-decolourised whole leaf extract may be attributed to an epigenetic effect of the material under investigation.http://www.sciencedirect.com/science/article/pii/S2214750021001505Aloe plantGenotoxicityIn vivo alkaline comet assayHydroxyanthraceneDerivativesHerbal preparation |
| spellingShingle | Corrado L. Galli Serena Cinelli Paola Ciliutti Gloria Melzi Marina Marinovich Lack of in vivo genotoxic effect of dried whole Aloe ferox juice Toxicology Reports Aloe plant Genotoxicity In vivo alkaline comet assay Hydroxyanthracene Derivatives Herbal preparation |
| title | Lack of in vivo genotoxic effect of dried whole Aloe ferox juice |
| title_full | Lack of in vivo genotoxic effect of dried whole Aloe ferox juice |
| title_fullStr | Lack of in vivo genotoxic effect of dried whole Aloe ferox juice |
| title_full_unstemmed | Lack of in vivo genotoxic effect of dried whole Aloe ferox juice |
| title_short | Lack of in vivo genotoxic effect of dried whole Aloe ferox juice |
| title_sort | lack of in vivo genotoxic effect of dried whole aloe ferox juice |
| topic | Aloe plant Genotoxicity In vivo alkaline comet assay Hydroxyanthracene Derivatives Herbal preparation |
| url | http://www.sciencedirect.com/science/article/pii/S2214750021001505 |
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