Oral Administration of Thymoquinone Attenuates Diazinon-induced Renal Injury in Rat: The Involvement of Keap1/Nrf2/HO1/NQO Signaling Pathway
The most prevalent component of the volatile oil found in Nigella sativa seeds is thymoquinone (TQ). As well as being used as food supplements, the seeds and oil are also utilized in traditional medicine to treat a variety of ailments. The purpose of this investigation was to determine whether TQ c...
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| Format: | Article |
| Language: | English |
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Assiut University
2023-09-01
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| Series: | Journal of Advanced Veterinary Research |
| Subjects: | |
| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1463 |
| _version_ | 1797657137802903552 |
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| author | Eman M. Fath Hatem H. Bakery Ragab M. EL Shawarby Mohamed E.S. Abosalem Nesrine Ebrahim Ahmed Medhat Hegazy Samer S. Ibrahim |
| author_facet | Eman M. Fath Hatem H. Bakery Ragab M. EL Shawarby Mohamed E.S. Abosalem Nesrine Ebrahim Ahmed Medhat Hegazy Samer S. Ibrahim |
| author_sort | Eman M. Fath |
| collection | DOAJ |
| description |
The most prevalent component of the volatile oil found in Nigella sativa seeds is thymoquinone (TQ). As well as being used as food supplements, the seeds and oil are also utilized in traditional medicine to treat a variety of ailments. The purpose of this investigation was to determine whether TQ could protect rats from acute nephrotoxicity caused by diazinon (DZN). Six equal groups of thirty six adult’s male Wistar rats were created at random. Group 1 (G1) was maintained in typical control circumstances and given saline daily intragastric (IG) for 4 weeks; G2 was administered 0.1 mL olive oil IG for 4 weeks; G3 was administered 0.1 mL DMSO IG for 4 weeks; G4 was administered IG TQ at a dose of 10 mg/kg B.W. daily for 4 weeks; G5 was administered IG DZN at a dose of 15 mg/kg B.W. daily for 4 weeks; G6 was administered IG TQ daily one hour before DZN at the same dose in G4 and G5 for 4 weeks. The findings shown that TQ reduces the renal dysfunctions brought on by DZN by restoring urea and creatinine levels as well as oxidative indicators. Although the expression of Keap-1 was also elevated, overexpression of Nrf2 also enhanced the expression of heme oxygenase-1 (HO-1), and NFκB in renal tissue. Also, TQ increased antiapoptotic (BCL2) factors and decrease proapoptotic (BAX) factors. As a result, it is hypothesized that TQ may be helpful in the prevention and management of acute nephrotoxicity brought on by DZN.
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| first_indexed | 2024-03-11T17:40:02Z |
| format | Article |
| id | doaj.art-ac23f884f939414580b74d66ef2b68a3 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-03-11T17:40:02Z |
| publishDate | 2023-09-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-ac23f884f939414580b74d66ef2b68a32023-10-18T14:07:56ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772023-09-01138Oral Administration of Thymoquinone Attenuates Diazinon-induced Renal Injury in Rat: The Involvement of Keap1/Nrf2/HO1/NQO Signaling PathwayEman M. Fath0Hatem H. Bakery1Ragab M. EL Shawarby2Mohamed E.S. Abosalem3Nesrine Ebrahim4Ahmed Medhat Hegazy5Samer S. Ibrahim6Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Qalyubia, Egypt.Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Qalyubia, Egypt.Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Qalyubia, Egypt.Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Qalyubia, Egypt.Department of Histology and Cell Biology Faculty of Medicine, Benha University, Benha 13511, Egypt.Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Qalyubia, Egypt.Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Qalyubia, Egypt. The most prevalent component of the volatile oil found in Nigella sativa seeds is thymoquinone (TQ). As well as being used as food supplements, the seeds and oil are also utilized in traditional medicine to treat a variety of ailments. The purpose of this investigation was to determine whether TQ could protect rats from acute nephrotoxicity caused by diazinon (DZN). Six equal groups of thirty six adult’s male Wistar rats were created at random. Group 1 (G1) was maintained in typical control circumstances and given saline daily intragastric (IG) for 4 weeks; G2 was administered 0.1 mL olive oil IG for 4 weeks; G3 was administered 0.1 mL DMSO IG for 4 weeks; G4 was administered IG TQ at a dose of 10 mg/kg B.W. daily for 4 weeks; G5 was administered IG DZN at a dose of 15 mg/kg B.W. daily for 4 weeks; G6 was administered IG TQ daily one hour before DZN at the same dose in G4 and G5 for 4 weeks. The findings shown that TQ reduces the renal dysfunctions brought on by DZN by restoring urea and creatinine levels as well as oxidative indicators. Although the expression of Keap-1 was also elevated, overexpression of Nrf2 also enhanced the expression of heme oxygenase-1 (HO-1), and NFκB in renal tissue. Also, TQ increased antiapoptotic (BCL2) factors and decrease proapoptotic (BAX) factors. As a result, it is hypothesized that TQ may be helpful in the prevention and management of acute nephrotoxicity brought on by DZN. https://advetresearch.com/index.php/AVR/article/view/1463 DiazinonN-acetyl cysteineNephrotoxicityNLRP3Oxidative stress |
| spellingShingle | Eman M. Fath Hatem H. Bakery Ragab M. EL Shawarby Mohamed E.S. Abosalem Nesrine Ebrahim Ahmed Medhat Hegazy Samer S. Ibrahim Oral Administration of Thymoquinone Attenuates Diazinon-induced Renal Injury in Rat: The Involvement of Keap1/Nrf2/HO1/NQO Signaling Pathway Journal of Advanced Veterinary Research Diazinon N-acetyl cysteine Nephrotoxicity NLRP3 Oxidative stress |
| title | Oral Administration of Thymoquinone Attenuates Diazinon-induced Renal Injury in Rat: The Involvement of Keap1/Nrf2/HO1/NQO Signaling Pathway |
| title_full | Oral Administration of Thymoquinone Attenuates Diazinon-induced Renal Injury in Rat: The Involvement of Keap1/Nrf2/HO1/NQO Signaling Pathway |
| title_fullStr | Oral Administration of Thymoquinone Attenuates Diazinon-induced Renal Injury in Rat: The Involvement of Keap1/Nrf2/HO1/NQO Signaling Pathway |
| title_full_unstemmed | Oral Administration of Thymoquinone Attenuates Diazinon-induced Renal Injury in Rat: The Involvement of Keap1/Nrf2/HO1/NQO Signaling Pathway |
| title_short | Oral Administration of Thymoquinone Attenuates Diazinon-induced Renal Injury in Rat: The Involvement of Keap1/Nrf2/HO1/NQO Signaling Pathway |
| title_sort | oral administration of thymoquinone attenuates diazinon induced renal injury in rat the involvement of keap1 nrf2 ho1 nqo signaling pathway |
| topic | Diazinon N-acetyl cysteine Nephrotoxicity NLRP3 Oxidative stress |
| url | https://advetresearch.com/index.php/AVR/article/view/1463 |
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