Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness

Human anthrax cases necessitate rapid response. We completed Bacillus anthracis nanopore whole-genome sequencing in our high-containment laboratory from a human anthrax isolate hours after receipt. The de novo assembled genome showed no evidence of known antimicrobial resistance genes or introduced...

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Main Authors: Heather P. McLaughlin, Julia V. Bugrysheva, Andrew B. Conley, Christopher A. Gulvik, Blake Cherney, Cari B. Kolton, Chung K. Marston, Elke Saile, Erin Swaney, David Lonsway, Amy S. Gargis, Thiphasone Kongphet-Tran, Christine Lascols, Pierre Michel, Julie Villanueva, Alex R. Hoffmaster, Jay E. Gee, David Sue
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2020-02-01
Series:Emerging Infectious Diseases
Subjects:
Online Access:https://wwwnc.cdc.gov/eid/article/26/2/19-1351_article
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author Heather P. McLaughlin
Julia V. Bugrysheva
Andrew B. Conley
Christopher A. Gulvik
Blake Cherney
Cari B. Kolton
Chung K. Marston
Elke Saile
Erin Swaney
David Lonsway
Amy S. Gargis
Thiphasone Kongphet-Tran
Christine Lascols
Pierre Michel
Julie Villanueva
Alex R. Hoffmaster
Jay E. Gee
David Sue
author_facet Heather P. McLaughlin
Julia V. Bugrysheva
Andrew B. Conley
Christopher A. Gulvik
Blake Cherney
Cari B. Kolton
Chung K. Marston
Elke Saile
Erin Swaney
David Lonsway
Amy S. Gargis
Thiphasone Kongphet-Tran
Christine Lascols
Pierre Michel
Julie Villanueva
Alex R. Hoffmaster
Jay E. Gee
David Sue
author_sort Heather P. McLaughlin
collection DOAJ
description Human anthrax cases necessitate rapid response. We completed Bacillus anthracis nanopore whole-genome sequencing in our high-containment laboratory from a human anthrax isolate hours after receipt. The de novo assembled genome showed no evidence of known antimicrobial resistance genes or introduced plasmid(s). Same-day genomic characterization enhances public health emergency response.
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spelling doaj.art-ac2475ca9ed343f6be9c7ddc7aad2b582022-12-22T03:14:49ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592020-02-0126235836110.3201/eid2602.191351Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency PreparednessHeather P. McLaughlinJulia V. BugryshevaAndrew B. ConleyChristopher A. GulvikBlake CherneyCari B. KoltonChung K. MarstonElke SaileErin SwaneyDavid LonswayAmy S. GargisThiphasone Kongphet-TranChristine LascolsPierre MichelJulie VillanuevaAlex R. HoffmasterJay E. GeeDavid SueHuman anthrax cases necessitate rapid response. We completed Bacillus anthracis nanopore whole-genome sequencing in our high-containment laboratory from a human anthrax isolate hours after receipt. The de novo assembled genome showed no evidence of known antimicrobial resistance genes or introduced plasmid(s). Same-day genomic characterization enhances public health emergency response.https://wwwnc.cdc.gov/eid/article/26/2/19-1351_articleanthraxBacillus anthracisbacteriawhole-genome sequencingnanoporeemergency preparedness
spellingShingle Heather P. McLaughlin
Julia V. Bugrysheva
Andrew B. Conley
Christopher A. Gulvik
Blake Cherney
Cari B. Kolton
Chung K. Marston
Elke Saile
Erin Swaney
David Lonsway
Amy S. Gargis
Thiphasone Kongphet-Tran
Christine Lascols
Pierre Michel
Julie Villanueva
Alex R. Hoffmaster
Jay E. Gee
David Sue
Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
Emerging Infectious Diseases
anthrax
Bacillus anthracis
bacteria
whole-genome sequencing
nanopore
emergency preparedness
title Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
title_full Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
title_fullStr Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
title_full_unstemmed Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
title_short Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
title_sort rapid nanopore whole genome sequencing for anthrax emergency preparedness
topic anthrax
Bacillus anthracis
bacteria
whole-genome sequencing
nanopore
emergency preparedness
url https://wwwnc.cdc.gov/eid/article/26/2/19-1351_article
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