Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil
Abstract Arthropod-borne pathogens are medically important because of their ability to cause diseases in their hosts. The purpose of this study was to detect the occurrence of Ehrlichia spp., piroplasmids and Hepatozoon spp. in dogs with anemia and thrombocytopenia in southern Brazil. EDTA-whole bl...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Parasitologia Veterinaria
2018-11-01
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000400505&lng=en&tlng=en |
| _version_ | 1818752854110765056 |
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| author | Anna Cláudia Baumel Mongruel Priscila Ikeda Keyla Carstens Marques de Sousa Jyan Lucas Benevenute Margarete Kimie Falbo Rosangela Zacarias Machado Adriano de Oliveira Torres Carrasco Marcos Rogério André Meire Christina Seki |
| author_facet | Anna Cláudia Baumel Mongruel Priscila Ikeda Keyla Carstens Marques de Sousa Jyan Lucas Benevenute Margarete Kimie Falbo Rosangela Zacarias Machado Adriano de Oliveira Torres Carrasco Marcos Rogério André Meire Christina Seki |
| author_sort | Anna Cláudia Baumel Mongruel |
| collection | DOAJ |
| description | Abstract Arthropod-borne pathogens are medically important because of their ability to cause diseases in their hosts. The purpose of this study was to detect the occurrence of Ehrlichia spp., piroplasmids and Hepatozoon spp. in dogs with anemia and thrombocytopenia in southern Brazil. EDTA-whole blood was collected from 75 domestic dogs presenting anemia or/and thrombocytopenia from Guarapuava, state of Paraná, Brazil. DNA samples were subjected to conventional PCR assays for Ehrlichia spp. (dsb), piroplasmids (18S rRNA) and Hepatozoon spp. (18S rRNA), followed by sequencing and phylogenetic analyses. Among the 75 dogs, one (1.33%) was positive for Hepatozoon sp. and six (8%) were positive for piroplasmids in 18S rRNA cPCR assays. None of the dogs showed positive results in Ehrlichia spp.-cPCR targeting dsb gene. The phylogenetic analyses revealed that three piroplasm sequences were clustered with Rangellia vitalii, while one sequence was grouped with B. vogeli. The only sequence obtained from Hepatozoon spp.-PCR protocol was pooled with H. canis. Therefore, there is urgent need for differential molecular diagnosis of the two piroplasm species cited as etiological agents in clinical cases of canine hemoparasitic diseases, given the higher pathogenic potential of R. vitalii than of B. vogeli. |
| first_indexed | 2024-12-18T04:58:04Z |
| format | Article |
| id | doaj.art-ac25213c6f2c4a3280ad6bce4fbb49c1 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-18T04:58:04Z |
| publishDate | 2018-11-01 |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-ac25213c6f2c4a3280ad6bce4fbb49c12022-12-21T21:20:14ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612018-11-0127450551310.1590/s1984-296120180069S1984-29612018000400505Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern BrazilAnna Cláudia Baumel MongruelPriscila IkedaKeyla Carstens Marques de SousaJyan Lucas BenevenuteMargarete Kimie FalboRosangela Zacarias MachadoAdriano de Oliveira Torres CarrascoMarcos Rogério AndréMeire Christina SekiAbstract Arthropod-borne pathogens are medically important because of their ability to cause diseases in their hosts. The purpose of this study was to detect the occurrence of Ehrlichia spp., piroplasmids and Hepatozoon spp. in dogs with anemia and thrombocytopenia in southern Brazil. EDTA-whole blood was collected from 75 domestic dogs presenting anemia or/and thrombocytopenia from Guarapuava, state of Paraná, Brazil. DNA samples were subjected to conventional PCR assays for Ehrlichia spp. (dsb), piroplasmids (18S rRNA) and Hepatozoon spp. (18S rRNA), followed by sequencing and phylogenetic analyses. Among the 75 dogs, one (1.33%) was positive for Hepatozoon sp. and six (8%) were positive for piroplasmids in 18S rRNA cPCR assays. None of the dogs showed positive results in Ehrlichia spp.-cPCR targeting dsb gene. The phylogenetic analyses revealed that three piroplasm sequences were clustered with Rangellia vitalii, while one sequence was grouped with B. vogeli. The only sequence obtained from Hepatozoon spp.-PCR protocol was pooled with H. canis. Therefore, there is urgent need for differential molecular diagnosis of the two piroplasm species cited as etiological agents in clinical cases of canine hemoparasitic diseases, given the higher pathogenic potential of R. vitalii than of B. vogeli.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000400505&lng=en&tlng=enBabesia vogeliRangelia vitaliiHepatozoon canisphylogenetic analysistick borne diseases |
| spellingShingle | Anna Cláudia Baumel Mongruel Priscila Ikeda Keyla Carstens Marques de Sousa Jyan Lucas Benevenute Margarete Kimie Falbo Rosangela Zacarias Machado Adriano de Oliveira Torres Carrasco Marcos Rogério André Meire Christina Seki Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil Revista Brasileira de Parasitologia Veterinária Babesia vogeli Rangelia vitalii Hepatozoon canis phylogenetic analysis tick borne diseases |
| title | Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil |
| title_full | Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil |
| title_fullStr | Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil |
| title_full_unstemmed | Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil |
| title_short | Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil |
| title_sort | molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern brazil |
| topic | Babesia vogeli Rangelia vitalii Hepatozoon canis phylogenetic analysis tick borne diseases |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000400505&lng=en&tlng=en |
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