Tumor microenvironment-responsive gold nanodendrites for nanoprobe-based single-cell Raman imaging and tumor-targeted chemo-photothermal therapy
Nanodendrite particles (NDs) with densely branched structures and biomimetic architectures have exhibited great promise in tumor therapy owing to their prolonged in vivo circulation time and exceptional photothermal efficiency. Nevertheless, traditional NDs are deficient in terms of specific surface...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2023-01-01
|
| Series: | Smart Materials in Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590183423000194 |
| _version_ | 1797774832568369152 |
|---|---|
| author | Yajun Shuai Qing Bao Hui Yue Jie Wang Tao Yang Quan Wan Yuxin Zhong Zongpu Xu Chuanbin Mao Mingying Yang |
| author_facet | Yajun Shuai Qing Bao Hui Yue Jie Wang Tao Yang Quan Wan Yuxin Zhong Zongpu Xu Chuanbin Mao Mingying Yang |
| author_sort | Yajun Shuai |
| collection | DOAJ |
| description | Nanodendrite particles (NDs) with densely branched structures and biomimetic architectures have exhibited great promise in tumor therapy owing to their prolonged in vivo circulation time and exceptional photothermal efficiency. Nevertheless, traditional NDs are deficient in terms of specific surface modification and targeting tumors, which restrict their potential for broader clinical applications. Here, we developed coronavirus-like gold NDs through a seed-mediated approach and using silk fibroin (SF) as a capping agent. Our results demonstrate that these NDs have a favorable drug-loading capacity (∼65.25%) and light-triggered release characteristics of doxorubicin hydrochloride (DOX). Additionally, NDs functionalized with specific probes exhibited exceptional surface-enhanced Raman scattering (SERS) characteristics, enabling high-sensitivity Raman imaging of unstained single cells. Moreover, these NDs allowed for real-time monitoring of endocytic NDs for over 24 h. Furthermore, ND@DOX conjugated with tumor-targeting peptides exhibited mild hyperthermia, minimal cytotoxicity, and effective targeting towards cancer cells in vitro, as well as responsiveness to the tumor microenvironment (TME) in vivo. These unique properties led to the highest level of synergistic tumor-killing efficiency when stimulated by a near-infrared (NIR) laser at 808 nm. Therefore, our virus-like ND functionalized with SF presents a novel type of nanocarrier that exhibits significant potential for synergistic applications in precision medicine. |
| first_indexed | 2024-03-12T22:25:57Z |
| format | Article |
| id | doaj.art-ac256f7877994f9092c58236f319e520 |
| institution | Directory Open Access Journal |
| issn | 2590-1834 |
| language | English |
| last_indexed | 2024-03-12T22:25:57Z |
| publishDate | 2023-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Smart Materials in Medicine |
| spelling | doaj.art-ac256f7877994f9092c58236f319e5202023-07-22T04:52:49ZengKeAi Communications Co., Ltd.Smart Materials in Medicine2590-18342023-01-014680689Tumor microenvironment-responsive gold nanodendrites for nanoprobe-based single-cell Raman imaging and tumor-targeted chemo-photothermal therapyYajun Shuai0Qing Bao1Hui Yue2Jie Wang3Tao Yang4Quan Wan5Yuxin Zhong6Zongpu Xu7Chuanbin Mao8Mingying Yang9Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Hangzhou, 310058, PR ChinaSchool of Materials Science and Engineering, Zhejiang University, Hangzhou, 310027, PR ChinaSchool of Materials Science and Engineering, Zhejiang University, Hangzhou, 310027, PR ChinaKey Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Hangzhou, 310058, PR ChinaSchool of Materials Science and Engineering, Zhejiang University, Hangzhou, 310027, PR ChinaKey Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Hangzhou, 310058, PR ChinaSchool of Materials Science and Engineering, Zhejiang University, Hangzhou, 310027, PR ChinaKey Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Hangzhou, 310058, PR ChinaSchool of Materials Science and Engineering, Zhejiang University, Hangzhou, 310027, PR China; Department of Biomedical Engineering, The Chinese University of Hong Kong, Sha Tin, Hong Kong SAR, PR China; Corresponding author. School of Materials Science and Engineering, Zhejiang University, Hangzhou, 310027, PR China.Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Hangzhou, 310058, PR China; Corresponding author.Nanodendrite particles (NDs) with densely branched structures and biomimetic architectures have exhibited great promise in tumor therapy owing to their prolonged in vivo circulation time and exceptional photothermal efficiency. Nevertheless, traditional NDs are deficient in terms of specific surface modification and targeting tumors, which restrict their potential for broader clinical applications. Here, we developed coronavirus-like gold NDs through a seed-mediated approach and using silk fibroin (SF) as a capping agent. Our results demonstrate that these NDs have a favorable drug-loading capacity (∼65.25%) and light-triggered release characteristics of doxorubicin hydrochloride (DOX). Additionally, NDs functionalized with specific probes exhibited exceptional surface-enhanced Raman scattering (SERS) characteristics, enabling high-sensitivity Raman imaging of unstained single cells. Moreover, these NDs allowed for real-time monitoring of endocytic NDs for over 24 h. Furthermore, ND@DOX conjugated with tumor-targeting peptides exhibited mild hyperthermia, minimal cytotoxicity, and effective targeting towards cancer cells in vitro, as well as responsiveness to the tumor microenvironment (TME) in vivo. These unique properties led to the highest level of synergistic tumor-killing efficiency when stimulated by a near-infrared (NIR) laser at 808 nm. Therefore, our virus-like ND functionalized with SF presents a novel type of nanocarrier that exhibits significant potential for synergistic applications in precision medicine.http://www.sciencedirect.com/science/article/pii/S2590183423000194Gold nanoparticlesSilk fibroinSERS imagingTargeted therapyMild photothermal heatingMultimodal bioimaging |
| spellingShingle | Yajun Shuai Qing Bao Hui Yue Jie Wang Tao Yang Quan Wan Yuxin Zhong Zongpu Xu Chuanbin Mao Mingying Yang Tumor microenvironment-responsive gold nanodendrites for nanoprobe-based single-cell Raman imaging and tumor-targeted chemo-photothermal therapy Smart Materials in Medicine Gold nanoparticles Silk fibroin SERS imaging Targeted therapy Mild photothermal heating Multimodal bioimaging |
| title | Tumor microenvironment-responsive gold nanodendrites for nanoprobe-based single-cell Raman imaging and tumor-targeted chemo-photothermal therapy |
| title_full | Tumor microenvironment-responsive gold nanodendrites for nanoprobe-based single-cell Raman imaging and tumor-targeted chemo-photothermal therapy |
| title_fullStr | Tumor microenvironment-responsive gold nanodendrites for nanoprobe-based single-cell Raman imaging and tumor-targeted chemo-photothermal therapy |
| title_full_unstemmed | Tumor microenvironment-responsive gold nanodendrites for nanoprobe-based single-cell Raman imaging and tumor-targeted chemo-photothermal therapy |
| title_short | Tumor microenvironment-responsive gold nanodendrites for nanoprobe-based single-cell Raman imaging and tumor-targeted chemo-photothermal therapy |
| title_sort | tumor microenvironment responsive gold nanodendrites for nanoprobe based single cell raman imaging and tumor targeted chemo photothermal therapy |
| topic | Gold nanoparticles Silk fibroin SERS imaging Targeted therapy Mild photothermal heating Multimodal bioimaging |
| url | http://www.sciencedirect.com/science/article/pii/S2590183423000194 |
| work_keys_str_mv | AT yajunshuai tumormicroenvironmentresponsivegoldnanodendritesfornanoprobebasedsinglecellramanimagingandtumortargetedchemophotothermaltherapy AT qingbao tumormicroenvironmentresponsivegoldnanodendritesfornanoprobebasedsinglecellramanimagingandtumortargetedchemophotothermaltherapy AT huiyue tumormicroenvironmentresponsivegoldnanodendritesfornanoprobebasedsinglecellramanimagingandtumortargetedchemophotothermaltherapy AT jiewang tumormicroenvironmentresponsivegoldnanodendritesfornanoprobebasedsinglecellramanimagingandtumortargetedchemophotothermaltherapy AT taoyang tumormicroenvironmentresponsivegoldnanodendritesfornanoprobebasedsinglecellramanimagingandtumortargetedchemophotothermaltherapy AT quanwan tumormicroenvironmentresponsivegoldnanodendritesfornanoprobebasedsinglecellramanimagingandtumortargetedchemophotothermaltherapy AT yuxinzhong tumormicroenvironmentresponsivegoldnanodendritesfornanoprobebasedsinglecellramanimagingandtumortargetedchemophotothermaltherapy AT zongpuxu tumormicroenvironmentresponsivegoldnanodendritesfornanoprobebasedsinglecellramanimagingandtumortargetedchemophotothermaltherapy AT chuanbinmao tumormicroenvironmentresponsivegoldnanodendritesfornanoprobebasedsinglecellramanimagingandtumortargetedchemophotothermaltherapy AT mingyingyang tumormicroenvironmentresponsivegoldnanodendritesfornanoprobebasedsinglecellramanimagingandtumortargetedchemophotothermaltherapy |