Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS
Tabat Barito (Ficus deltoidea) is known as a plant that has potency as an antioxidant because of containing a significant phenolic compound. In this study, we performed metabolite profiling on F. deltoidea leaves by UPLC-QTOF-MS/MS to identify its phenolic compounds. Extraction by maceration and ult...
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| פורמט: | Article |
| שפה: | English |
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University of Brawijaya
2018-05-01
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| סדרה: | Journal of Pure and Applied Chemistry Research |
| נושאים: | |
| גישה מקוונת: | https://jpacr.ub.ac.id/index.php/jpacr/article/view/374/pdf |
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| author | Nurmaida Nurmaida Latifah K Darusman Mohamad Rafi Rudi Heryanto |
| author_facet | Nurmaida Nurmaida Latifah K Darusman Mohamad Rafi Rudi Heryanto |
| author_sort | Nurmaida Nurmaida |
| collection | DOAJ |
| description | Tabat Barito (Ficus deltoidea) is known as a plant that has potency as an antioxidant because of containing a significant phenolic compound. In this study, we performed metabolite profiling on F. deltoidea leaves by UPLC-QTOF-MS/MS to identify its phenolic compounds. Extraction by maceration and ultrasonication techniques with methanol (MM and UM) and ethanol (ME and UE) were used to extract the F. deltoidea metabolites. About 70 metabolites were identified by using UPLC-QTOF-MS/MS in negative ion mode. The amounts of metabolites found in each extract were different, i.e., 45 metabolites in MM, 64 metabolites in UM, 42 metabolites in UE and 41 metabolites in ME. MS/MS could further tentatively identify 16 metabolites. The identified compounds belonged to the class of flavonoids and phenolic acid. Also, we conducted an antioxidant activity by using DPPH method on each extract to determine its potency as an antioxidant. The highest antioxidant activity was exhibited by UM extract (IC50 71.93 ppm) may be due to the number of metabolites in UM extract which was higher than the other extract based on the detected metabolites. |
| first_indexed | 2024-12-18T22:24:22Z |
| format | Article |
| id | doaj.art-ac2578c3f2a54b9f94ebb51df98b2f14 |
| institution | Directory Open Access Journal |
| issn | 2302-4690 2302-4690 |
| language | English |
| last_indexed | 2024-12-18T22:24:22Z |
| publishDate | 2018-05-01 |
| publisher | University of Brawijaya |
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| series | Journal of Pure and Applied Chemistry Research |
| spelling | doaj.art-ac2578c3f2a54b9f94ebb51df98b2f142022-12-21T20:49:36ZengUniversity of BrawijayaJournal of Pure and Applied Chemistry Research2302-46902302-46902018-05-0172100108http://dx.doi.org/10.21776/ub.jpacr.2018.007.02.374Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MSNurmaida Nurmaida0Latifah K Darusman1Mohamad Rafi2Rudi Heryanto3Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia and Center of Standard Test of Agricultural Quarantine, Indonesia Agricultural Quarantine Agency, Ministry of Agriculture Republic of Indonesia, Jl. Pemuda No. 64 Kav. 16-17, Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia and Tropical Biopharmaca Research Center, Bogor Agricultural University, Jl. Taman Kencana No. 3 Kampus IPB Taman Kencana, Bogor 16128, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia and Tropical Biopharmaca Research Center, Bogor Agricultural University, Jl. Taman Kencana No. 3 Kampus IPB Taman Kencana, Bogor 16128, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia and Tropical Biopharmaca Research Center, Bogor Agricultural University, Jl. Taman Kencana No. 3 Kampus IPB Taman Kencana, Bogor 16128, IndonesiaTabat Barito (Ficus deltoidea) is known as a plant that has potency as an antioxidant because of containing a significant phenolic compound. In this study, we performed metabolite profiling on F. deltoidea leaves by UPLC-QTOF-MS/MS to identify its phenolic compounds. Extraction by maceration and ultrasonication techniques with methanol (MM and UM) and ethanol (ME and UE) were used to extract the F. deltoidea metabolites. About 70 metabolites were identified by using UPLC-QTOF-MS/MS in negative ion mode. The amounts of metabolites found in each extract were different, i.e., 45 metabolites in MM, 64 metabolites in UM, 42 metabolites in UE and 41 metabolites in ME. MS/MS could further tentatively identify 16 metabolites. The identified compounds belonged to the class of flavonoids and phenolic acid. Also, we conducted an antioxidant activity by using DPPH method on each extract to determine its potency as an antioxidant. The highest antioxidant activity was exhibited by UM extract (IC50 71.93 ppm) may be due to the number of metabolites in UM extract which was higher than the other extract based on the detected metabolites.https://jpacr.ub.ac.id/index.php/jpacr/article/view/374/pdfficus deltoideametabolite profilinguplc-qtof-ms/msantioxidant activity |
| spellingShingle | Nurmaida Nurmaida Latifah K Darusman Mohamad Rafi Rudi Heryanto Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS Journal of Pure and Applied Chemistry Research ficus deltoidea metabolite profiling uplc-qtof-ms/ms antioxidant activity |
| title | Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS |
| title_full | Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS |
| title_fullStr | Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS |
| title_full_unstemmed | Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS |
| title_short | Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS |
| title_sort | metabolite profiling of tabat barito ficus deltoidea using uplc qtof ms ms |
| topic | ficus deltoidea metabolite profiling uplc-qtof-ms/ms antioxidant activity |
| url | https://jpacr.ub.ac.id/index.php/jpacr/article/view/374/pdf |
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