Emergence of IncHI2 Plasmid-Harboring blaNDM-5 from Porcine <i>Escherichia coli</i> Isolates in Guangdong, China
Carbapenem resistance has posed potential harmful risks to human and animals. The objectives of this study were to understand the prevalence of <i>bla</i><sub>NDM-5</sub> in pigs and investigate the molecular characteristics of NDM-5-producing <i>Escherichia coli</i&...
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MDPI AG
2021-07-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/8/954 |
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| author | Zhenbao Ma Zhenling Zeng Jiao Liu Chang Liu Yu Pan Yanan Zhang Yafei Li |
| author_facet | Zhenbao Ma Zhenling Zeng Jiao Liu Chang Liu Yu Pan Yanan Zhang Yafei Li |
| author_sort | Zhenbao Ma |
| collection | DOAJ |
| description | Carbapenem resistance has posed potential harmful risks to human and animals. The objectives of this study were to understand the prevalence of <i>bla</i><sub>NDM-5</sub> in pigs and investigate the molecular characteristics of NDM-5-producing <i>Escherichia coli</i> isolates in Guangdong province in China. Carbapenem-resistant <i>E. coli</i> isolates were isolated from pigs and obtained using MacConkey plates containing 0.5 mg/L meropenem. Conjugation assay and antimicrobial susceptibility testing were conducted for the isolates and their transconjugants. Whole-genome sequence (WGS) was used to analyze the plasmid genetic feature. A total of five <i>bla</i><sub>NDM-5</sub>-carrying <i>E. coli</i> isolates were obtained in the present investigations. They belonged to five ST types. The <i>bla</i><sub>NDM-5</sub> genes were found to be in IncX3 and IncHI2 plasmid. The IncX3 plasmid was 46,161 bp in size and identical to other reports. IncHI2 plasmid was 246,593 bp in size and similar to other IncHI2-ST3 plasmids. It consisted of a typical IncHI2 plasmid backbone region and a multiresistance region (MRR). The <i>bla</i><sub>NDM-5</sub> was closely associated with the <i>IS3000-ISAba125-blaNDM-5-bleMBL-trpF-tat-IS26</i> unit. We first reported the <i>bla</i><sub>NDM-5</sub>-carrying IncHI2 in <i>E. coli</i> isolates recovered from pigs and revealed the molecular characterization. Continued surveillance for the dissemination of <i>bla</i><sub>NDM-5</sub> among food-producing animals is required. |
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| publishDate | 2021-07-01 |
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| spelling | doaj.art-ac2a036f8e7d46af8b57359c77f0d1042023-11-22T09:08:32ZengMDPI AGPathogens2076-08172021-07-0110895410.3390/pathogens10080954Emergence of IncHI2 Plasmid-Harboring blaNDM-5 from Porcine <i>Escherichia coli</i> Isolates in Guangdong, ChinaZhenbao Ma0Zhenling Zeng1Jiao Liu2Chang Liu3Yu Pan4Yanan Zhang5Yafei Li6Institute of Quality Standard and Monitoring Technology for Agro-Products, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang 550005, ChinaInstitute of Quality Standard and Monitoring Technology for Agro-Products, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaCarbapenem resistance has posed potential harmful risks to human and animals. The objectives of this study were to understand the prevalence of <i>bla</i><sub>NDM-5</sub> in pigs and investigate the molecular characteristics of NDM-5-producing <i>Escherichia coli</i> isolates in Guangdong province in China. Carbapenem-resistant <i>E. coli</i> isolates were isolated from pigs and obtained using MacConkey plates containing 0.5 mg/L meropenem. Conjugation assay and antimicrobial susceptibility testing were conducted for the isolates and their transconjugants. Whole-genome sequence (WGS) was used to analyze the plasmid genetic feature. A total of five <i>bla</i><sub>NDM-5</sub>-carrying <i>E. coli</i> isolates were obtained in the present investigations. They belonged to five ST types. The <i>bla</i><sub>NDM-5</sub> genes were found to be in IncX3 and IncHI2 plasmid. The IncX3 plasmid was 46,161 bp in size and identical to other reports. IncHI2 plasmid was 246,593 bp in size and similar to other IncHI2-ST3 plasmids. It consisted of a typical IncHI2 plasmid backbone region and a multiresistance region (MRR). The <i>bla</i><sub>NDM-5</sub> was closely associated with the <i>IS3000-ISAba125-blaNDM-5-bleMBL-trpF-tat-IS26</i> unit. We first reported the <i>bla</i><sub>NDM-5</sub>-carrying IncHI2 in <i>E. coli</i> isolates recovered from pigs and revealed the molecular characterization. Continued surveillance for the dissemination of <i>bla</i><sub>NDM-5</sub> among food-producing animals is required.https://www.mdpi.com/2076-0817/10/8/954<i>Escherichia coli</i><i>bla</i><sub>NDM-5</sub>IncHI2 plasmidresistance |
| spellingShingle | Zhenbao Ma Zhenling Zeng Jiao Liu Chang Liu Yu Pan Yanan Zhang Yafei Li Emergence of IncHI2 Plasmid-Harboring blaNDM-5 from Porcine <i>Escherichia coli</i> Isolates in Guangdong, China Pathogens <i>Escherichia coli</i> <i>bla</i><sub>NDM-5</sub> IncHI2 plasmid resistance |
| title | Emergence of IncHI2 Plasmid-Harboring blaNDM-5 from Porcine <i>Escherichia coli</i> Isolates in Guangdong, China |
| title_full | Emergence of IncHI2 Plasmid-Harboring blaNDM-5 from Porcine <i>Escherichia coli</i> Isolates in Guangdong, China |
| title_fullStr | Emergence of IncHI2 Plasmid-Harboring blaNDM-5 from Porcine <i>Escherichia coli</i> Isolates in Guangdong, China |
| title_full_unstemmed | Emergence of IncHI2 Plasmid-Harboring blaNDM-5 from Porcine <i>Escherichia coli</i> Isolates in Guangdong, China |
| title_short | Emergence of IncHI2 Plasmid-Harboring blaNDM-5 from Porcine <i>Escherichia coli</i> Isolates in Guangdong, China |
| title_sort | emergence of inchi2 plasmid harboring blandm 5 from porcine i escherichia coli i isolates in guangdong china |
| topic | <i>Escherichia coli</i> <i>bla</i><sub>NDM-5</sub> IncHI2 plasmid resistance |
| url | https://www.mdpi.com/2076-0817/10/8/954 |
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