Isolation of MRSA, ESBL and AmpC – β -lactamases from Neonatal Sepsis at a Tertiary Care Hospital
Background and Objectives: The emergence of methicillin resistant Staphylococcus aureus (MRSA) and extended spectrum β– lactamases (ESBLs) in neonatal intensive care unit patients is increasing. This study aims to find out the bacteriological profile in neonatal sepsis and study their antimicro...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2014-06-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/4512/8597_final(K)_PF1(AG)_F(DK)_PFA(AK)_PF2(PAG)_PF2(PN).pdf |
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| author | Amutha Chelliah Thyagarajan Ravinder Radhika Katragadda K.V. Leela R. Narayana Babu |
| author_facet | Amutha Chelliah Thyagarajan Ravinder Radhika Katragadda K.V. Leela R. Narayana Babu |
| author_sort | Amutha Chelliah |
| collection | DOAJ |
| description | Background and Objectives: The emergence of methicillin
resistant Staphylococcus aureus (MRSA) and extended spectrum
β– lactamases (ESBLs) in neonatal intensive care unit patients is
increasing.
This study aims to find out the bacteriological profile in neonatal
sepsis and study their antimicrobial susceptibility pattern
including detection of MRSA and ESBLs.
Materials and Methods: This study was conducted for a period
of one and a half years from January 2010 to June 2011 in a
tertiary care hospital in Chennai. A total of 182 blood samples
were collected using sterile precautions. They were processed
following standard laboratory protocol. Antibiogram was done
using appropriate antibiotics by Kirby-Bauer disc diffusion
method. Isolated Staphylococcus aureus were tested for
methicillin resistance using Cefoxitin disc (30µg), ESBL was
detected using combined disc method, MIC reduction and
Polymerase chain reaction, metallobetalactamases using EDTA
and Amp-C beta lactamases using AmpC disc test. C-reactive
protein (CRP) was estimated for all the cases.
Results: Out of the 182 cases, 110 (60.4%) were culture positive.
Fifty five (63.9 %) of early onset sepsis cases had Gram negative
bacteria (GNB) and 19 (79.1%) of late onset sepsis cases had
Gram positive bacteria. Out of the total pathogens, 31 (28.1%)
were Klebsiella pneumoniae and 30 (27%) were Staphylococcus
aureus.
17 (56.6 %) of Staphylococcus aureus were found to be MRSA
and they were 100% sensitive to Vancomycin. 33 (67.3%) of
Enterobacteriaceae were ESBL producers. ESBL isolates were
100% sensitive to Imipenem. Three (6.1%) of Enterobacteriaceae
were AmpC producers and 3 (27.2%) of Pseudomonas
aeruginosa were MBL producers. CRP was positive in 99 (54.3%)
cases, out of which 94 (94.9%) were culture positive.
Conclusion: Klebsiella spp. and Staphylococcus aureus were
the commonest bacteria causing neonatal sepsis in this centre.
Multidrug resistance among the isolates was common. Early
diagnosis and institution of specific antibiotics after studying the
sensitivity pattern will help in reducing neonatal morbidity and
mortality and prevent emergence of drug resistant strains. |
| first_indexed | 2024-12-19T00:03:04Z |
| format | Article |
| id | doaj.art-ac2da3f1b8b746dbb3771fa0e7ea6591 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-19T00:03:04Z |
| publishDate | 2014-06-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-ac2da3f1b8b746dbb3771fa0e7ea65912022-12-21T20:46:22ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-06-0186DC24DC2710.7860/JCDR/2014/8597.4512Isolation of MRSA, ESBL and AmpC – β -lactamases from Neonatal Sepsis at a Tertiary Care HospitalAmutha Chelliah0Thyagarajan Ravinder1Radhika Katragadda2K.V. Leela3R. Narayana Babu4Assistant Professor, Department of Microbiology, Govt. Kilpauk Medical College, Chennai, India.Professor, Department of Microbiology, Govt. Kilpauk Medical College, Chennai, India.Professor and HOD, Department of Microbiology, Govt. Kilpauk Medical College, Chennai, India.Associate Professor, Department of Microbiology, Govt. Kilpauk Medical College, Chennai, India.Professor and HOD, Department of Paediatrics, Govt. Kilpauk Medical College, Chennai, India.Background and Objectives: The emergence of methicillin resistant Staphylococcus aureus (MRSA) and extended spectrum β– lactamases (ESBLs) in neonatal intensive care unit patients is increasing. This study aims to find out the bacteriological profile in neonatal sepsis and study their antimicrobial susceptibility pattern including detection of MRSA and ESBLs. Materials and Methods: This study was conducted for a period of one and a half years from January 2010 to June 2011 in a tertiary care hospital in Chennai. A total of 182 blood samples were collected using sterile precautions. They were processed following standard laboratory protocol. Antibiogram was done using appropriate antibiotics by Kirby-Bauer disc diffusion method. Isolated Staphylococcus aureus were tested for methicillin resistance using Cefoxitin disc (30µg), ESBL was detected using combined disc method, MIC reduction and Polymerase chain reaction, metallobetalactamases using EDTA and Amp-C beta lactamases using AmpC disc test. C-reactive protein (CRP) was estimated for all the cases. Results: Out of the 182 cases, 110 (60.4%) were culture positive. Fifty five (63.9 %) of early onset sepsis cases had Gram negative bacteria (GNB) and 19 (79.1%) of late onset sepsis cases had Gram positive bacteria. Out of the total pathogens, 31 (28.1%) were Klebsiella pneumoniae and 30 (27%) were Staphylococcus aureus. 17 (56.6 %) of Staphylococcus aureus were found to be MRSA and they were 100% sensitive to Vancomycin. 33 (67.3%) of Enterobacteriaceae were ESBL producers. ESBL isolates were 100% sensitive to Imipenem. Three (6.1%) of Enterobacteriaceae were AmpC producers and 3 (27.2%) of Pseudomonas aeruginosa were MBL producers. CRP was positive in 99 (54.3%) cases, out of which 94 (94.9%) were culture positive. Conclusion: Klebsiella spp. and Staphylococcus aureus were the commonest bacteria causing neonatal sepsis in this centre. Multidrug resistance among the isolates was common. Early diagnosis and institution of specific antibiotics after studying the sensitivity pattern will help in reducing neonatal morbidity and mortality and prevent emergence of drug resistant strains.https://jcdr.net/articles/PDF/4512/8597_final(K)_PF1(AG)_F(DK)_PFA(AK)_PF2(PAG)_PF2(PN).pdfc-reactive proteinextended spectrum beta lactamasesklebsiella pneumoniaemethicillin resistantneonatal sepsisstaphylococcus aureus |
| spellingShingle | Amutha Chelliah Thyagarajan Ravinder Radhika Katragadda K.V. Leela R. Narayana Babu Isolation of MRSA, ESBL and AmpC – β -lactamases from Neonatal Sepsis at a Tertiary Care Hospital Journal of Clinical and Diagnostic Research c-reactive protein extended spectrum beta lactamases klebsiella pneumoniae methicillin resistant neonatal sepsis staphylococcus aureus |
| title | Isolation of MRSA, ESBL and AmpC – β -lactamases from Neonatal Sepsis at a Tertiary Care Hospital |
| title_full | Isolation of MRSA, ESBL and AmpC – β -lactamases from Neonatal Sepsis at a Tertiary Care Hospital |
| title_fullStr | Isolation of MRSA, ESBL and AmpC – β -lactamases from Neonatal Sepsis at a Tertiary Care Hospital |
| title_full_unstemmed | Isolation of MRSA, ESBL and AmpC – β -lactamases from Neonatal Sepsis at a Tertiary Care Hospital |
| title_short | Isolation of MRSA, ESBL and AmpC – β -lactamases from Neonatal Sepsis at a Tertiary Care Hospital |
| title_sort | isolation of mrsa esbl and ampc β lactamases from neonatal sepsis at a tertiary care hospital |
| topic | c-reactive protein extended spectrum beta lactamases klebsiella pneumoniae methicillin resistant neonatal sepsis staphylococcus aureus |
| url | https://jcdr.net/articles/PDF/4512/8597_final(K)_PF1(AG)_F(DK)_PFA(AK)_PF2(PAG)_PF2(PN).pdf |
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