In Vivo Production of Monoclonal Antibodies by Gene Transfer via Electroporation Protects against Lethal Influenza and Ebola Infections
Monoclonal antibodies (mAbs) have wide clinical utility, but global access is limited by high costs and impracticalities associated with repeated passive administration. Here, we describe an optimized electroporation-based DNA gene transfer platform technology that can be utilized for production of...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-12-01
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| Series: | Molecular Therapy: Methods & Clinical Development |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S232905011730102X |
| _version_ | 1828463905480900608 |
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| author | Chasity D. Andrews Yang Luo Ming Sun Jian Yu Arthur J. Goff Pamela J. Glass Neal N. Padte Yaoxing Huang David D. Ho |
| author_facet | Chasity D. Andrews Yang Luo Ming Sun Jian Yu Arthur J. Goff Pamela J. Glass Neal N. Padte Yaoxing Huang David D. Ho |
| author_sort | Chasity D. Andrews |
| collection | DOAJ |
| description | Monoclonal antibodies (mAbs) have wide clinical utility, but global access is limited by high costs and impracticalities associated with repeated passive administration. Here, we describe an optimized electroporation-based DNA gene transfer platform technology that can be utilized for production of functional mAbs in vivo, with the potential to reduce costs and administration burdens. We demonstrate that multiple mAbs can be simultaneously expressed at protective concentrations for a protracted period of time using DNA doses and electroporation conditions that are feasible clinically. The expressed mAbs could also protect mice against lethal influenza or Ebola virus challenges. Our findings suggest that this DNA gene transfer platform technology could be a game-changing advance that expands access to effective mAb therapeutics globally. |
| first_indexed | 2024-12-11T03:02:13Z |
| format | Article |
| id | doaj.art-ac310ef9b08343a4956a922f3c9d2077 |
| institution | Directory Open Access Journal |
| issn | 2329-0501 |
| language | English |
| last_indexed | 2024-12-11T03:02:13Z |
| publishDate | 2017-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Methods & Clinical Development |
| spelling | doaj.art-ac310ef9b08343a4956a922f3c9d20772022-12-22T01:23:02ZengElsevierMolecular Therapy: Methods & Clinical Development2329-05012017-12-017C748210.1016/j.omtm.2017.09.003In Vivo Production of Monoclonal Antibodies by Gene Transfer via Electroporation Protects against Lethal Influenza and Ebola InfectionsChasity D. Andrews0Yang Luo1Ming Sun2Jian Yu3Arthur J. Goff4Pamela J. Glass5Neal N. Padte6Yaoxing Huang7David D. Ho8Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USAAaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USAAaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USAAaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USAUS Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USAUS Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USAAaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USAAaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USAAaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USAMonoclonal antibodies (mAbs) have wide clinical utility, but global access is limited by high costs and impracticalities associated with repeated passive administration. Here, we describe an optimized electroporation-based DNA gene transfer platform technology that can be utilized for production of functional mAbs in vivo, with the potential to reduce costs and administration burdens. We demonstrate that multiple mAbs can be simultaneously expressed at protective concentrations for a protracted period of time using DNA doses and electroporation conditions that are feasible clinically. The expressed mAbs could also protect mice against lethal influenza or Ebola virus challenges. Our findings suggest that this DNA gene transfer platform technology could be a game-changing advance that expands access to effective mAb therapeutics globally.http://www.sciencedirect.com/science/article/pii/S232905011730102XplasmidDNA-based antibody gene transferelectroporationinfectious diseaseinfluenzaEbola |
| spellingShingle | Chasity D. Andrews Yang Luo Ming Sun Jian Yu Arthur J. Goff Pamela J. Glass Neal N. Padte Yaoxing Huang David D. Ho In Vivo Production of Monoclonal Antibodies by Gene Transfer via Electroporation Protects against Lethal Influenza and Ebola Infections Molecular Therapy: Methods & Clinical Development plasmid DNA-based antibody gene transfer electroporation infectious disease influenza Ebola |
| title | In Vivo Production of Monoclonal Antibodies by Gene Transfer via Electroporation Protects against Lethal Influenza and Ebola Infections |
| title_full | In Vivo Production of Monoclonal Antibodies by Gene Transfer via Electroporation Protects against Lethal Influenza and Ebola Infections |
| title_fullStr | In Vivo Production of Monoclonal Antibodies by Gene Transfer via Electroporation Protects against Lethal Influenza and Ebola Infections |
| title_full_unstemmed | In Vivo Production of Monoclonal Antibodies by Gene Transfer via Electroporation Protects against Lethal Influenza and Ebola Infections |
| title_short | In Vivo Production of Monoclonal Antibodies by Gene Transfer via Electroporation Protects against Lethal Influenza and Ebola Infections |
| title_sort | in vivo production of monoclonal antibodies by gene transfer via electroporation protects against lethal influenza and ebola infections |
| topic | plasmid DNA-based antibody gene transfer electroporation infectious disease influenza Ebola |
| url | http://www.sciencedirect.com/science/article/pii/S232905011730102X |
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