Protective effect of rosiglitazone on microscopic and oxidative stress parameters of ram sperm after freeze-thawing
Abstract The purpose of this study was to investigate the effects of rosiglitazone on ram semen after cryopreservation on the quality of thawed sperm. Sperm motility, membrane functionality, viability, total abnormality, acrosome membrane integrity, mitochondrial activity, reactive oxygen species pr...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-18298-2 |
| _version_ | 1818517090580037632 |
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| author | Mahdieh Mehdipour Hossein Daghigh-Kia Abouzar Najafi Zohreh Mehdipour Hossein Mohammadi |
| author_facet | Mahdieh Mehdipour Hossein Daghigh-Kia Abouzar Najafi Zohreh Mehdipour Hossein Mohammadi |
| author_sort | Mahdieh Mehdipour |
| collection | DOAJ |
| description | Abstract The purpose of this study was to investigate the effects of rosiglitazone on ram semen after cryopreservation on the quality of thawed sperm. Sperm motility, membrane functionality, viability, total abnormality, acrosome membrane integrity, mitochondrial activity, reactive oxygen species production, ATP content and apoptotic features were assessed after thawing. Rosiglitazone at concentration of 60 µM resulted in the highest (P < 0.05) total motility, progressive motility and straight-line velocity. The percentages of average path velocity and curvilinear velocity were greater in the 60 µM group. Different concentrations of rosiglitazone did not have significant effects on amplitude of the lateral head displacement, linearity and straightness. The highest amounts of membrane functionality and mitochondrial activity after freeze-thawing were observed in groups containing 60 µM. By increasing the rosiglitazone level to 80 µM, no positive effect was observed in most of the evaluated parameters. The lowest ROS concentration was recorded in 60 µM rosiglitazone group (P < 0.05). The group containing 60 µM rosiglitazone also produced the lowest significant percentage of apoptosis-like changes and dead sperm. A greater (P < 0.05) percentage of acrosome integrity in frozen-thawed spermatozoa was observed in the 60 µM rosiglitazone group. There was no significant difference between 40 and 60 µM rosiglitazone in intact acrosome of ram thawed semen. The result showed that supplementation in ram semen extender with rosiglitazone had a positive role in the regulation of ram sperm motility and had strong protective effect on the sperm membrane and acrosome integrity. |
| first_indexed | 2024-12-11T00:51:32Z |
| format | Article |
| id | doaj.art-ac31dcec30554aa5b19f17d7d7c656a4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-11T00:51:32Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ac31dcec30554aa5b19f17d7d7c656a42022-12-22T01:26:37ZengNature PortfolioScientific Reports2045-23222022-08-0112111010.1038/s41598-022-18298-2Protective effect of rosiglitazone on microscopic and oxidative stress parameters of ram sperm after freeze-thawingMahdieh Mehdipour0Hossein Daghigh-Kia1Abouzar Najafi2Zohreh Mehdipour3Hossein Mohammadi4Department of Animal Science, College of Agriculture, University of TabrizDepartment of Animal Science, College of Agriculture, University of TabrizDepartment of Animal and Poultry Science, College of Aburaihan, University of TehranPrestage Department of Poultry Science, North Carolina State UniversityDepartment of Animal Science, Faculty of Agriculture and Natural Resources, Arak UniversityAbstract The purpose of this study was to investigate the effects of rosiglitazone on ram semen after cryopreservation on the quality of thawed sperm. Sperm motility, membrane functionality, viability, total abnormality, acrosome membrane integrity, mitochondrial activity, reactive oxygen species production, ATP content and apoptotic features were assessed after thawing. Rosiglitazone at concentration of 60 µM resulted in the highest (P < 0.05) total motility, progressive motility and straight-line velocity. The percentages of average path velocity and curvilinear velocity were greater in the 60 µM group. Different concentrations of rosiglitazone did not have significant effects on amplitude of the lateral head displacement, linearity and straightness. The highest amounts of membrane functionality and mitochondrial activity after freeze-thawing were observed in groups containing 60 µM. By increasing the rosiglitazone level to 80 µM, no positive effect was observed in most of the evaluated parameters. The lowest ROS concentration was recorded in 60 µM rosiglitazone group (P < 0.05). The group containing 60 µM rosiglitazone also produced the lowest significant percentage of apoptosis-like changes and dead sperm. A greater (P < 0.05) percentage of acrosome integrity in frozen-thawed spermatozoa was observed in the 60 µM rosiglitazone group. There was no significant difference between 40 and 60 µM rosiglitazone in intact acrosome of ram thawed semen. The result showed that supplementation in ram semen extender with rosiglitazone had a positive role in the regulation of ram sperm motility and had strong protective effect on the sperm membrane and acrosome integrity.https://doi.org/10.1038/s41598-022-18298-2 |
| spellingShingle | Mahdieh Mehdipour Hossein Daghigh-Kia Abouzar Najafi Zohreh Mehdipour Hossein Mohammadi Protective effect of rosiglitazone on microscopic and oxidative stress parameters of ram sperm after freeze-thawing Scientific Reports |
| title | Protective effect of rosiglitazone on microscopic and oxidative stress parameters of ram sperm after freeze-thawing |
| title_full | Protective effect of rosiglitazone on microscopic and oxidative stress parameters of ram sperm after freeze-thawing |
| title_fullStr | Protective effect of rosiglitazone on microscopic and oxidative stress parameters of ram sperm after freeze-thawing |
| title_full_unstemmed | Protective effect of rosiglitazone on microscopic and oxidative stress parameters of ram sperm after freeze-thawing |
| title_short | Protective effect of rosiglitazone on microscopic and oxidative stress parameters of ram sperm after freeze-thawing |
| title_sort | protective effect of rosiglitazone on microscopic and oxidative stress parameters of ram sperm after freeze thawing |
| url | https://doi.org/10.1038/s41598-022-18298-2 |
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