Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells
Background: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal...
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| Format: | Article |
| Language: | English |
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Universidad de Antioquia
2020-09-01
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| Series: | Revista Colombiana de Ciencias Pecuarias |
| Subjects: | |
| Online Access: | https://revistas.udea.edu.co/index.php/rccp/article/view/343953 |
| _version_ | 1827584417921499136 |
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| author | Pablo E. Ocampo Viviana Vallejo Luis M. Montoya Noeme S. Rocha Fernanda da C. Landim Sheila C. Rahal |
| author_facet | Pablo E. Ocampo Viviana Vallejo Luis M. Montoya Noeme S. Rocha Fernanda da C. Landim Sheila C. Rahal |
| author_sort | Pablo E. Ocampo |
| collection | DOAJ |
| description | Background: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs). Methods: MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry. Results: The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs. Conclusions: Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM. |
| first_indexed | 2024-03-08T23:30:03Z |
| format | Article |
| id | doaj.art-ac359c6d52d846f4a3bcbffe4dc77ed9 |
| institution | Directory Open Access Journal |
| issn | 2256-2958 |
| language | English |
| last_indexed | 2024-03-08T23:30:03Z |
| publishDate | 2020-09-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Revista Colombiana de Ciencias Pecuarias |
| spelling | doaj.art-ac359c6d52d846f4a3bcbffe4dc77ed92023-12-14T15:34:28ZengUniversidad de AntioquiaRevista Colombiana de Ciencias Pecuarias2256-29582020-09-0134321222310.17533/udea.rccp.v34n3a0641366Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cellsPablo E. Ocampo0Viviana Vallejo1Luis M. Montoya2Noeme S. Rocha3Fernanda da C. Landim4Sheila C. Rahal5São Paulo State UniversitySão Paulo State UniversityNational University of ColombiaSão Paulo State UniversitySão Paulo State UniversitySão Paulo State UniversityBackground: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs). Methods: MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry. Results: The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs. Conclusions: Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM.https://revistas.udea.edu.co/index.php/rccp/article/view/343953cell differentiationcartilagechondrocyteschondrogenesischondrogenic differentiationcollagencorticosteroidsglucocorticoidhistologyhyaluronic acidmesenchymal stem cellosteoarthritistriancinolone acetonideviscosupplementation |
| spellingShingle | Pablo E. Ocampo Viviana Vallejo Luis M. Montoya Noeme S. Rocha Fernanda da C. Landim Sheila C. Rahal Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells Revista Colombiana de Ciencias Pecuarias cell differentiation cartilage chondrocytes chondrogenesis chondrogenic differentiation collagen corticosteroids glucocorticoid histology hyaluronic acid mesenchymal stem cell osteoarthritis triancinolone acetonide viscosupplementation |
| title | Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells |
| title_full | Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells |
| title_fullStr | Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells |
| title_full_unstemmed | Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells |
| title_short | Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells |
| title_sort | potential effect of hyaluronic acid and triamcinolone acetate alone or combined on chondrogenic differentiation of mesenchymal stem cells |
| topic | cell differentiation cartilage chondrocytes chondrogenesis chondrogenic differentiation collagen corticosteroids glucocorticoid histology hyaluronic acid mesenchymal stem cell osteoarthritis triancinolone acetonide viscosupplementation |
| url | https://revistas.udea.edu.co/index.php/rccp/article/view/343953 |
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