In vitro anti-aging activity of Muntingia calabura L. fruit extract and its fractions
Context: Premature aging usually occurred due to free radicals reducing the skins’ physiological functions. Muntingia calabura, a plant containing rich antioxidants, has the potential to overcome this problem. Aims: To evaluate the antioxidant capacity of M. calabura in inhibiting the premature a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2021-07-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | https://jppres.com/jppres/pdf/vol9/jppres20.979_9.4.409.pdf |
| _version_ | 1818385425734041600 |
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| author | Syamsu Nur Aprilia Angreiny Angelina Muhammad Aswad Risfah Yulianty Asril Burhan Nursamsiar |
| author_facet | Syamsu Nur Aprilia Angreiny Angelina Muhammad Aswad Risfah Yulianty Asril Burhan Nursamsiar |
| author_sort | Syamsu Nur |
| collection | DOAJ |
| description | Context: Premature aging usually occurred due to free radicals reducing the skins’ physiological functions. Muntingia calabura, a plant containing rich antioxidants, has the potential to overcome this problem.
Aims: To evaluate the antioxidant capacity of M. calabura in inhibiting the premature aging process, to be potentially developed into an antiaging active ingredient.
Methods: The samples were extracted using ethanol 96%, and processed into n-hexane, ethyl acetate, and ethanol fractions, respectively. Total phenolic content was determined, followed by the evaluation of antioxidant capacity through DPPH, FRAP, and ABTS assay. Further, anti-elastase was conducted using human neutrophil elastase as a skin degradation enzyme, followed by an anti-collagenase test. Finally, normal cell proliferation was also evaluated via the MTT method measuring cell viability on HDFa cells.
Results: As the results, ethanol extract, ethyl acetate fraction, and ethanol fraction showed a strong antioxidant effect, having great capacity reducing DPPH, ABTS radicals, and also iron reduction, in contrast to n-hexane fraction that exhibited only weak activity. The antioxidant trend capacities were found directly correlated to total phenolic contents. Furthermore, the ethyl acetate fraction was found to have optimum activity in inhibiting elastase and collagenase enzymes, showing a similar impact on cell viability.
Conclusions: The ethyl acetate fraction from M. calabura exhibits the prospect for further development to support its effectiveness as an active ingredient in antiaging cosmetics. |
| first_indexed | 2024-12-14T03:37:57Z |
| format | Article |
| id | doaj.art-ac38965f21334791a0987b656fd9f4d2 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-12-14T03:37:57Z |
| publishDate | 2021-07-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-ac38965f21334791a0987b656fd9f4d22022-12-21T23:18:33ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502021-07-0194409421In vitro anti-aging activity of Muntingia calabura L. fruit extract and its fractionsSyamsu Nur0Aprilia Angreiny Angelina1Muhammad Aswad2Risfah Yulianty3Asril Burhan4Nursamsiar5Department of Pharmaceutical Chemistry, Sekolah Tinggi Ilmu Farmasi, Makassar, 90242, Indonesia.Department of Pharmaceutical Chemistry, Sekolah Tinggi Ilmu Farmasi, Makassar, 90242, Indonesia.Pharmacy Faculty, Hasanuddin University, Makassar, 90242, Indonesia.Pharmacy Faculty, Hasanuddin University, Makassar, 90242, Indonesia.Department of Pharmaceutical Chemistry, Sekolah Tinggi Ilmu Farmasi, Makassar, 90242, Indonesia.Department of Pharmaceutical Chemistry, Sekolah Tinggi Ilmu Farmasi, Makassar, 90242, Indonesia.Context: Premature aging usually occurred due to free radicals reducing the skins’ physiological functions. Muntingia calabura, a plant containing rich antioxidants, has the potential to overcome this problem. Aims: To evaluate the antioxidant capacity of M. calabura in inhibiting the premature aging process, to be potentially developed into an antiaging active ingredient. Methods: The samples were extracted using ethanol 96%, and processed into n-hexane, ethyl acetate, and ethanol fractions, respectively. Total phenolic content was determined, followed by the evaluation of antioxidant capacity through DPPH, FRAP, and ABTS assay. Further, anti-elastase was conducted using human neutrophil elastase as a skin degradation enzyme, followed by an anti-collagenase test. Finally, normal cell proliferation was also evaluated via the MTT method measuring cell viability on HDFa cells. Results: As the results, ethanol extract, ethyl acetate fraction, and ethanol fraction showed a strong antioxidant effect, having great capacity reducing DPPH, ABTS radicals, and also iron reduction, in contrast to n-hexane fraction that exhibited only weak activity. The antioxidant trend capacities were found directly correlated to total phenolic contents. Furthermore, the ethyl acetate fraction was found to have optimum activity in inhibiting elastase and collagenase enzymes, showing a similar impact on cell viability. Conclusions: The ethyl acetate fraction from M. calabura exhibits the prospect for further development to support its effectiveness as an active ingredient in antiaging cosmetics.https://jppres.com/jppres/pdf/vol9/jppres20.979_9.4.409.pdfantiagingantioxidantcell viabilitycosmeticmuntingia calabura l. |
| spellingShingle | Syamsu Nur Aprilia Angreiny Angelina Muhammad Aswad Risfah Yulianty Asril Burhan Nursamsiar In vitro anti-aging activity of Muntingia calabura L. fruit extract and its fractions Journal of Pharmacy & Pharmacognosy Research antiaging antioxidant cell viability cosmetic muntingia calabura l. |
| title | In vitro anti-aging activity of Muntingia calabura L. fruit extract and its fractions |
| title_full | In vitro anti-aging activity of Muntingia calabura L. fruit extract and its fractions |
| title_fullStr | In vitro anti-aging activity of Muntingia calabura L. fruit extract and its fractions |
| title_full_unstemmed | In vitro anti-aging activity of Muntingia calabura L. fruit extract and its fractions |
| title_short | In vitro anti-aging activity of Muntingia calabura L. fruit extract and its fractions |
| title_sort | in vitro anti aging activity of muntingia calabura l fruit extract and its fractions |
| topic | antiaging antioxidant cell viability cosmetic muntingia calabura l. |
| url | https://jppres.com/jppres/pdf/vol9/jppres20.979_9.4.409.pdf |
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