Heparin-binding protein in ventilator-induced lung injury
Abstract Background Although mechanical ventilation is often lifesaving, it can also cause injury to the lungs. The lung injury is caused by not only high pressure and mechanical forces but also by inflammatory processes that are not fully understood. Heparin-binding protein (HBP), released by activ...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-09-01
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| Series: | Intensive Care Medicine Experimental |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40635-018-0198-x |
| _version_ | 1828875326341513216 |
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| author | Jonas Tydén N. Larsson S. Lehtipalo H. Herwald M. Hultin J. Walldén A. F. Behndig J. Johansson |
| author_facet | Jonas Tydén N. Larsson S. Lehtipalo H. Herwald M. Hultin J. Walldén A. F. Behndig J. Johansson |
| author_sort | Jonas Tydén |
| collection | DOAJ |
| description | Abstract Background Although mechanical ventilation is often lifesaving, it can also cause injury to the lungs. The lung injury is caused by not only high pressure and mechanical forces but also by inflammatory processes that are not fully understood. Heparin-binding protein (HBP), released by activated granulocytes, has been indicated as a possible mediator of increased vascular permeability in the lung injury associated with trauma and sepsis. We investigated if HBP levels were increased in the bronchoalveolar lavage fluid (BALF) or plasma in a pig model of ventilator-induced lung injury (VILI). We also investigated if HBP was present in BALF from healthy volunteers and in intubated patients in the intensive care unit (ICU). Methods Anaesthetized pigs were randomized to receive ventilation with either tidal volumes of 8 ml/kg (controls, n = 6) or 20 ml/kg (VILI group, n = 6). Plasma and BALF samples were taken at 0, 1, 2, 4, and 6 h. In humans, HBP levels in BALF were sampled from 16 healthy volunteers and from 10 intubated patients being cared for in the ICU. Results Plasma levels of HBP did not differ between pigs in the control and VILI groups. The median HBP levels in BALF were higher in the VILI group after 6 h of ventilation compared to those in the controls (1144 ng/ml (IQR 359–1636 ng/ml) versus 89 ng/ml (IQR 33–191 ng/ml) ng/ml, respectively, p = 0.02). The median HBP level in BALF from healthy volunteers was 0.90 ng/ml (IQR 0.79–1.01 ng/ml) as compared to 1959 ng/ml (IQR 612–3306 ng/ml) from intubated ICU patients (p < 0.001). Conclusions In a model of VILI in pigs, levels of HBP in BALF increased over time compared to controls, while plasma levels did not differ between the two groups. HBP in BALF was high in intubated ICU patients in spite of the seemingly non-harmful ventilation, suggesting that inflammation from other causes might increase HBP levels. |
| first_indexed | 2024-12-13T07:56:58Z |
| format | Article |
| id | doaj.art-ac3960202e2e46eca3122ad2783309cc |
| institution | Directory Open Access Journal |
| issn | 2197-425X |
| language | English |
| last_indexed | 2024-12-13T07:56:58Z |
| publishDate | 2018-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Intensive Care Medicine Experimental |
| spelling | doaj.art-ac3960202e2e46eca3122ad2783309cc2022-12-21T23:54:31ZengSpringerOpenIntensive Care Medicine Experimental2197-425X2018-09-016111110.1186/s40635-018-0198-xHeparin-binding protein in ventilator-induced lung injuryJonas Tydén0N. Larsson1S. Lehtipalo2H. Herwald3M. Hultin4J. Walldén5A. F. Behndig6J. Johansson7Department of Surgical and Perioperative Sciences, Anaesthesiology and Critical Care Medicine (Östersund), Umeå UniversityDepartment of Surgical and Perioperative Sciences, Anaesthesiology and Critical Care Medicine (Umeå), Umeå UniversityDepartment of Surgical and Perioperative Sciences, Anaesthesiology and Critical Care Medicine (Umeå), Umeå UniversityDepartment of Cell and Molecular Biology, Lund UniversityDepartment of Surgical and Perioperative Sciences, Anaesthesiology and Critical Care Medicine (Umeå), Umeå UniversityDepartment of Surgical and Perioperative Sciences, Anaesthesiology and Critical Care Medicine (Sundsvall), Umeå UniversityDepartment of Public Health and Clinical Medicine, Division of Medicine, Umeå UniversityDepartment of Surgical and Perioperative Sciences, Anaesthesiology and Critical Care Medicine (Östersund), Umeå UniversityAbstract Background Although mechanical ventilation is often lifesaving, it can also cause injury to the lungs. The lung injury is caused by not only high pressure and mechanical forces but also by inflammatory processes that are not fully understood. Heparin-binding protein (HBP), released by activated granulocytes, has been indicated as a possible mediator of increased vascular permeability in the lung injury associated with trauma and sepsis. We investigated if HBP levels were increased in the bronchoalveolar lavage fluid (BALF) or plasma in a pig model of ventilator-induced lung injury (VILI). We also investigated if HBP was present in BALF from healthy volunteers and in intubated patients in the intensive care unit (ICU). Methods Anaesthetized pigs were randomized to receive ventilation with either tidal volumes of 8 ml/kg (controls, n = 6) or 20 ml/kg (VILI group, n = 6). Plasma and BALF samples were taken at 0, 1, 2, 4, and 6 h. In humans, HBP levels in BALF were sampled from 16 healthy volunteers and from 10 intubated patients being cared for in the ICU. Results Plasma levels of HBP did not differ between pigs in the control and VILI groups. The median HBP levels in BALF were higher in the VILI group after 6 h of ventilation compared to those in the controls (1144 ng/ml (IQR 359–1636 ng/ml) versus 89 ng/ml (IQR 33–191 ng/ml) ng/ml, respectively, p = 0.02). The median HBP level in BALF from healthy volunteers was 0.90 ng/ml (IQR 0.79–1.01 ng/ml) as compared to 1959 ng/ml (IQR 612–3306 ng/ml) from intubated ICU patients (p < 0.001). Conclusions In a model of VILI in pigs, levels of HBP in BALF increased over time compared to controls, while plasma levels did not differ between the two groups. HBP in BALF was high in intubated ICU patients in spite of the seemingly non-harmful ventilation, suggesting that inflammation from other causes might increase HBP levels.http://link.springer.com/article/10.1186/s40635-018-0198-xVentilator-induced lung injuryHBPPigsNeutrophils |
| spellingShingle | Jonas Tydén N. Larsson S. Lehtipalo H. Herwald M. Hultin J. Walldén A. F. Behndig J. Johansson Heparin-binding protein in ventilator-induced lung injury Intensive Care Medicine Experimental Ventilator-induced lung injury HBP Pigs Neutrophils |
| title | Heparin-binding protein in ventilator-induced lung injury |
| title_full | Heparin-binding protein in ventilator-induced lung injury |
| title_fullStr | Heparin-binding protein in ventilator-induced lung injury |
| title_full_unstemmed | Heparin-binding protein in ventilator-induced lung injury |
| title_short | Heparin-binding protein in ventilator-induced lung injury |
| title_sort | heparin binding protein in ventilator induced lung injury |
| topic | Ventilator-induced lung injury HBP Pigs Neutrophils |
| url | http://link.springer.com/article/10.1186/s40635-018-0198-x |
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