Development and evaluation of an immunochromatography-based point-of-care test kit for a rapid diagnosis of human cysticercosis
Human cysticercosis is a life-threatening zoonotic disease caused by infection with larvae (cysticerci) of the pork tapeworm, Taenia solium. This can affect the nervous system causing chronic headache and intracranial hypertension, potentially leading to epileptic seizures and paralysis. The disease...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Food and Waterborne Parasitology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405676623000240 |
| _version_ | 1797398582592012288 |
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| author | Lakkhana Sadaow Patcharaporn Boonroumkaew Rutchanee Rodpai Penchom Janwan Oranuch Sanpool Tongjit Thanchomnang Yasuyuki Morishima Marcello Otake Sato Yasuhito Sako Kaoru Kobayashi Misako Iwai Wanchai Maleewong Hiroshi Yamasaki Pewpan M. Intapan |
| author_facet | Lakkhana Sadaow Patcharaporn Boonroumkaew Rutchanee Rodpai Penchom Janwan Oranuch Sanpool Tongjit Thanchomnang Yasuyuki Morishima Marcello Otake Sato Yasuhito Sako Kaoru Kobayashi Misako Iwai Wanchai Maleewong Hiroshi Yamasaki Pewpan M. Intapan |
| author_sort | Lakkhana Sadaow |
| collection | DOAJ |
| description | Human cysticercosis is a life-threatening zoonotic disease caused by infection with larvae (cysticerci) of the pork tapeworm, Taenia solium. This can affect the nervous system causing chronic headache and intracranial hypertension, potentially leading to epileptic seizures and paralysis. The disease is found in developing countries, especially in Southeast and South Asia, Sub-Saharan Africa, and Central and South America where porcine cysticercosis is endemic and people have a habit of eating undercooked pork. An immunochromatography-based test (ICT) kit, using T. solium cyst fluid as antigen, was manufactured to detect anti-T. solium IgG antibodies in human serum. To evaluate the kit, we used 187 serum samples including 24 from proven/confirmed cysticercosis cases, 133 from cases with other parasitosis and 30 healthy controls. Diagnostic efficiencies were calculated. The sensitivity, specificity, and accuracy were 83.3%, 92.0%, and 90.9%, respectively. Moreover, the ICT was positive before treatment but became negative after treatment, implying that this kit is also useful for follow-up monitoring post-treatment. In conclusion, we have successfully developed and present preliminary evaluation of an easy-to-handle rapid diagnostic tool for human cysticercosis in the form of an ICT platform using as antigen fluid from T. solium cysticerci. |
| first_indexed | 2024-03-09T01:27:35Z |
| format | Article |
| id | doaj.art-ac3d537af6b9416bbf1f38da18518034 |
| institution | Directory Open Access Journal |
| issn | 2405-6766 |
| language | English |
| last_indexed | 2024-03-09T01:27:35Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food and Waterborne Parasitology |
| spelling | doaj.art-ac3d537af6b9416bbf1f38da185180342023-12-10T06:16:39ZengElsevierFood and Waterborne Parasitology2405-67662023-12-0133e00211Development and evaluation of an immunochromatography-based point-of-care test kit for a rapid diagnosis of human cysticercosisLakkhana Sadaow0Patcharaporn Boonroumkaew1Rutchanee Rodpai2Penchom Janwan3Oranuch Sanpool4Tongjit Thanchomnang5Yasuyuki Morishima6Marcello Otake Sato7Yasuhito Sako8Kaoru Kobayashi9Misako Iwai10Wanchai Maleewong11Hiroshi Yamasaki12Pewpan M. Intapan13Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandMekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandMekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandMekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand; Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, ThailandMekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandMekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand; Department of Parasitology, Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, ThailandDepartment of Parasitology, National Institute of Infectious Diseases, Tokyo 162-8640, JapanDivision of Global Environment Parasitology, Faculty of Medical Technology, Niigata University of Pharmacy and Medical and Life Sciences, Niigata 956-8603, JapanDivision of Parasitology, Department of Infectious Diseases, Asahikawa Medical University, Asahikawa 078-8510, JapanDivision of Planning and Development, Adtec Inc., Oita 879-0453, JapanDivision of Planning and Development, Adtec Inc., Oita 879-0453, JapanMekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Parasitology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; Corresponding author at: Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; Corresponding author at: Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand.Human cysticercosis is a life-threatening zoonotic disease caused by infection with larvae (cysticerci) of the pork tapeworm, Taenia solium. This can affect the nervous system causing chronic headache and intracranial hypertension, potentially leading to epileptic seizures and paralysis. The disease is found in developing countries, especially in Southeast and South Asia, Sub-Saharan Africa, and Central and South America where porcine cysticercosis is endemic and people have a habit of eating undercooked pork. An immunochromatography-based test (ICT) kit, using T. solium cyst fluid as antigen, was manufactured to detect anti-T. solium IgG antibodies in human serum. To evaluate the kit, we used 187 serum samples including 24 from proven/confirmed cysticercosis cases, 133 from cases with other parasitosis and 30 healthy controls. Diagnostic efficiencies were calculated. The sensitivity, specificity, and accuracy were 83.3%, 92.0%, and 90.9%, respectively. Moreover, the ICT was positive before treatment but became negative after treatment, implying that this kit is also useful for follow-up monitoring post-treatment. In conclusion, we have successfully developed and present preliminary evaluation of an easy-to-handle rapid diagnostic tool for human cysticercosis in the form of an ICT platform using as antigen fluid from T. solium cysticerci.http://www.sciencedirect.com/science/article/pii/S2405676623000240Human cysticercosisTaenia soliumImmunochromatographyAntibody detectionDiagnosticsA point-of-care test |
| spellingShingle | Lakkhana Sadaow Patcharaporn Boonroumkaew Rutchanee Rodpai Penchom Janwan Oranuch Sanpool Tongjit Thanchomnang Yasuyuki Morishima Marcello Otake Sato Yasuhito Sako Kaoru Kobayashi Misako Iwai Wanchai Maleewong Hiroshi Yamasaki Pewpan M. Intapan Development and evaluation of an immunochromatography-based point-of-care test kit for a rapid diagnosis of human cysticercosis Food and Waterborne Parasitology Human cysticercosis Taenia solium Immunochromatography Antibody detection Diagnostics A point-of-care test |
| title | Development and evaluation of an immunochromatography-based point-of-care test kit for a rapid diagnosis of human cysticercosis |
| title_full | Development and evaluation of an immunochromatography-based point-of-care test kit for a rapid diagnosis of human cysticercosis |
| title_fullStr | Development and evaluation of an immunochromatography-based point-of-care test kit for a rapid diagnosis of human cysticercosis |
| title_full_unstemmed | Development and evaluation of an immunochromatography-based point-of-care test kit for a rapid diagnosis of human cysticercosis |
| title_short | Development and evaluation of an immunochromatography-based point-of-care test kit for a rapid diagnosis of human cysticercosis |
| title_sort | development and evaluation of an immunochromatography based point of care test kit for a rapid diagnosis of human cysticercosis |
| topic | Human cysticercosis Taenia solium Immunochromatography Antibody detection Diagnostics A point-of-care test |
| url | http://www.sciencedirect.com/science/article/pii/S2405676623000240 |
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