Initial Weight Loss, Anthropometric Parameters, and Proinflammatory Transcript Levels in Patients with Class I Obesity
Research into early predictors of effective weight loss could help determine more effective therapeutic interventions. In this study, 106 subjects with class I obesity, genotyped with the fat mass and obesity-associated (<i>FTO</i>) rs9930506 gene variant, were enrolled into a 12-week we...
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MDPI AG
2023-08-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/8/2304 |
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| author | Beata Jabłonowska-Lietz Grażyna Nowicka Marta Włodarczyk Sławomir Rejowski Maria Stasiowska Małgorzata Wrzosek |
| author_facet | Beata Jabłonowska-Lietz Grażyna Nowicka Marta Włodarczyk Sławomir Rejowski Maria Stasiowska Małgorzata Wrzosek |
| author_sort | Beata Jabłonowska-Lietz |
| collection | DOAJ |
| description | Research into early predictors of effective weight loss could help determine more effective therapeutic interventions. In this study, 106 subjects with class I obesity, genotyped with the fat mass and obesity-associated (<i>FTO</i>) rs9930506 gene variant, were enrolled into a 12-week weight loss program (WLP). Anthropometric and body composition measurements were controlled with bioelectrical impedance analysis (BIA) at baseline and after 4 and 12 weeks. Biopsies of abdominal subcutaneous adipose tissue (AT) and venous blood samples were collected to monitor changes in interleukin 6 (<i>IL-6</i>), tumor necrosis factor-alpha (<i>TNF-α</i>), and nuclear factor kappa B (<i>NF-κB</i>) mRNA levels in white blood cells (WBCs) and to assess if changes in WBC gene expression reflected changes in adipose tissue. The <i>FTO</i> rs9930506 variant had no effect on weight loss and no reduction in proinflammatory transcripts in WBCs or AT. Changes in anthropometric parameters were associated with changes in carbohydrate metabolism. A linear regression model showed that initial weight loss (after 4 weeks of the WLP) was the most predictive factor of weight loss success after 12 weeks of the WLP. Changes in plasma lipids or proinflammatory transcript levels in WBCs or AT were not associated with weight loss effectiveness. However, the gene expression in WBCs did reflect changes occurring in subcutaneous AT. |
| first_indexed | 2024-03-09T10:55:34Z |
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| id | doaj.art-ac404486a5914cc8a2c6bdabf612a703 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T10:55:34Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-ac404486a5914cc8a2c6bdabf612a7032023-12-01T01:42:25ZengMDPI AGBiomedicines2227-90592023-08-01118230410.3390/biomedicines11082304Initial Weight Loss, Anthropometric Parameters, and Proinflammatory Transcript Levels in Patients with Class I ObesityBeata Jabłonowska-Lietz0Grażyna Nowicka1Marta Włodarczyk2Sławomir Rejowski3Maria Stasiowska4Małgorzata Wrzosek5Medical Center, National Institute of Public Health NIH—National Research Institute, 24 Chocimska St., 00-791 Warsaw, PolandDepartment of Biochemistry and Pharmacogenomics, Center for Preclinical Research, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, PolandDepartment of Biochemistry and Pharmacogenomics, Center for Preclinical Research, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, PolandLiver and Internal Medicine Unit, Department of General, Transplant and Liver Surgery, Medical University of Warsaw, 1A Banacha St., 02-097 Warsaw, PolandDepartment of Anaesthesia and Intensive Care, University College London Hospital, National Hospital for Neurology and Neurosurgery, Queen Square, London WC1N 3BG, UKDepartment of Biochemistry and Pharmacogenomics, Center for Preclinical Research, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, PolandResearch into early predictors of effective weight loss could help determine more effective therapeutic interventions. In this study, 106 subjects with class I obesity, genotyped with the fat mass and obesity-associated (<i>FTO</i>) rs9930506 gene variant, were enrolled into a 12-week weight loss program (WLP). Anthropometric and body composition measurements were controlled with bioelectrical impedance analysis (BIA) at baseline and after 4 and 12 weeks. Biopsies of abdominal subcutaneous adipose tissue (AT) and venous blood samples were collected to monitor changes in interleukin 6 (<i>IL-6</i>), tumor necrosis factor-alpha (<i>TNF-α</i>), and nuclear factor kappa B (<i>NF-κB</i>) mRNA levels in white blood cells (WBCs) and to assess if changes in WBC gene expression reflected changes in adipose tissue. The <i>FTO</i> rs9930506 variant had no effect on weight loss and no reduction in proinflammatory transcripts in WBCs or AT. Changes in anthropometric parameters were associated with changes in carbohydrate metabolism. A linear regression model showed that initial weight loss (after 4 weeks of the WLP) was the most predictive factor of weight loss success after 12 weeks of the WLP. Changes in plasma lipids or proinflammatory transcript levels in WBCs or AT were not associated with weight loss effectiveness. However, the gene expression in WBCs did reflect changes occurring in subcutaneous AT.https://www.mdpi.com/2227-9059/11/8/2304weight loss predictorssubcutaneous adipose tissue<i>FTO</i> rs9930506 polymorphismproinflammatory gene expressionBIAHOMA-IR |
| spellingShingle | Beata Jabłonowska-Lietz Grażyna Nowicka Marta Włodarczyk Sławomir Rejowski Maria Stasiowska Małgorzata Wrzosek Initial Weight Loss, Anthropometric Parameters, and Proinflammatory Transcript Levels in Patients with Class I Obesity Biomedicines weight loss predictors subcutaneous adipose tissue <i>FTO</i> rs9930506 polymorphism proinflammatory gene expression BIA HOMA-IR |
| title | Initial Weight Loss, Anthropometric Parameters, and Proinflammatory Transcript Levels in Patients with Class I Obesity |
| title_full | Initial Weight Loss, Anthropometric Parameters, and Proinflammatory Transcript Levels in Patients with Class I Obesity |
| title_fullStr | Initial Weight Loss, Anthropometric Parameters, and Proinflammatory Transcript Levels in Patients with Class I Obesity |
| title_full_unstemmed | Initial Weight Loss, Anthropometric Parameters, and Proinflammatory Transcript Levels in Patients with Class I Obesity |
| title_short | Initial Weight Loss, Anthropometric Parameters, and Proinflammatory Transcript Levels in Patients with Class I Obesity |
| title_sort | initial weight loss anthropometric parameters and proinflammatory transcript levels in patients with class i obesity |
| topic | weight loss predictors subcutaneous adipose tissue <i>FTO</i> rs9930506 polymorphism proinflammatory gene expression BIA HOMA-IR |
| url | https://www.mdpi.com/2227-9059/11/8/2304 |
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