miR-200b inhibits prostate cancer EMT, growth and metastasis.
miRNA regulate gene expression at post-transcriptional level and fine-tune the key biological processes, including cancer progression. Here, we demonstrate the involvement of miR-200 b in the metastatic spread of prostate cancer. We identified miR-200 b as a downstream target of androgen receptor an...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3877136?pdf=render |
| _version_ | 1818270888369324032 |
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| author | LaTanya V Williams Dorina Veliceasa Elena Vinokour Olga V Volpert |
| author_facet | LaTanya V Williams Dorina Veliceasa Elena Vinokour Olga V Volpert |
| author_sort | LaTanya V Williams |
| collection | DOAJ |
| description | miRNA regulate gene expression at post-transcriptional level and fine-tune the key biological processes, including cancer progression. Here, we demonstrate the involvement of miR-200 b in the metastatic spread of prostate cancer. We identified miR-200 b as a downstream target of androgen receptor and linked its expression to decreased tumorigenicity and metastatic capacity of the prostate cancer cells. Overexpression of miR-200 b in PC-3 cells significantly inhibited their proliferation and the formation of subcutaneous tumors. Moreover, in an orthotopic model, miR-200 b blocked spontaneous metastasis and angiogenesis by PC-3 cells. This decreased metastatic potential was likely due to the reversal of the epithelial-to-mesenchymal transition, as was evidenced by increased pan-epithelial marker E-cadherin and specific markers of prostate epithelium, cytokeratins 8 and 18. In contrast, mesenchymal markers, fibronectin and vimentin, were significantly downregulated by miR-200 b. Our results suggest an important role for miR-200 b in prostate cancer progression and indicate its potential utility for prostate cancer therapy. |
| first_indexed | 2024-12-12T21:17:26Z |
| format | Article |
| id | doaj.art-ac40a7c17f6049a19e0d064ad7940afe |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T21:17:26Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ac40a7c17f6049a19e0d064ad7940afe2022-12-22T00:11:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8399110.1371/journal.pone.0083991miR-200b inhibits prostate cancer EMT, growth and metastasis.LaTanya V WilliamsDorina VeliceasaElena VinokourOlga V VolpertmiRNA regulate gene expression at post-transcriptional level and fine-tune the key biological processes, including cancer progression. Here, we demonstrate the involvement of miR-200 b in the metastatic spread of prostate cancer. We identified miR-200 b as a downstream target of androgen receptor and linked its expression to decreased tumorigenicity and metastatic capacity of the prostate cancer cells. Overexpression of miR-200 b in PC-3 cells significantly inhibited their proliferation and the formation of subcutaneous tumors. Moreover, in an orthotopic model, miR-200 b blocked spontaneous metastasis and angiogenesis by PC-3 cells. This decreased metastatic potential was likely due to the reversal of the epithelial-to-mesenchymal transition, as was evidenced by increased pan-epithelial marker E-cadherin and specific markers of prostate epithelium, cytokeratins 8 and 18. In contrast, mesenchymal markers, fibronectin and vimentin, were significantly downregulated by miR-200 b. Our results suggest an important role for miR-200 b in prostate cancer progression and indicate its potential utility for prostate cancer therapy.http://europepmc.org/articles/PMC3877136?pdf=render |
| spellingShingle | LaTanya V Williams Dorina Veliceasa Elena Vinokour Olga V Volpert miR-200b inhibits prostate cancer EMT, growth and metastasis. PLoS ONE |
| title | miR-200b inhibits prostate cancer EMT, growth and metastasis. |
| title_full | miR-200b inhibits prostate cancer EMT, growth and metastasis. |
| title_fullStr | miR-200b inhibits prostate cancer EMT, growth and metastasis. |
| title_full_unstemmed | miR-200b inhibits prostate cancer EMT, growth and metastasis. |
| title_short | miR-200b inhibits prostate cancer EMT, growth and metastasis. |
| title_sort | mir 200b inhibits prostate cancer emt growth and metastasis |
| url | http://europepmc.org/articles/PMC3877136?pdf=render |
| work_keys_str_mv | AT latanyavwilliams mir200binhibitsprostatecanceremtgrowthandmetastasis AT dorinaveliceasa mir200binhibitsprostatecanceremtgrowthandmetastasis AT elenavinokour mir200binhibitsprostatecanceremtgrowthandmetastasis AT olgavvolpert mir200binhibitsprostatecanceremtgrowthandmetastasis |