YWHAE promotes proliferation, metastasis, and chemoresistance in breast cancer cells
Abstract Breast cancer is the most common female‐specific malignancy in Taiwan and developed countries worldwide, and its incidence continues to grow. 14‐3‐3ε (YWHAE), which belong to 14‐3‐3 family, it has been reported up‐regulated in breast cancer tissues. However, the clinical implication and fun...
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| Format: | Article |
| Language: | English |
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Wiley
2019-07-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12075 |
| _version_ | 1819210137904087040 |
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| author | Yi‐Fang Yang Yi‐Chen Lee Yen‐Yun Wang Chie‐Hong Wang Ming‐Feng Hou Shyng‐Shiou F. Yuan |
| author_facet | Yi‐Fang Yang Yi‐Chen Lee Yen‐Yun Wang Chie‐Hong Wang Ming‐Feng Hou Shyng‐Shiou F. Yuan |
| author_sort | Yi‐Fang Yang |
| collection | DOAJ |
| description | Abstract Breast cancer is the most common female‐specific malignancy in Taiwan and developed countries worldwide, and its incidence continues to grow. 14‐3‐3ε (YWHAE), which belong to 14‐3‐3 family, it has been reported up‐regulated in breast cancer tissues. However, the clinical implication and function of YWHAE in breast cancer remains unclear. In this study, we investigated the prognostic value of the YWHAE in human breast cancer. Immunohistochemistry was used to analyze YWHAE expression in breast cancer tissues. Cell model was applied to examine the functions of YWHAE. The chemotherapeutic agents were used to evaluate the effect of YWHAE in breast cancer cell lines. YWHAE expression was associated with tumor size, lymph node metastasis, and poor patient survival in patients with breast cancer. YWHAE overexpression significantly increased the proliferation, migration, and invasion abilities of breast cancer cells. Knockdown of YWHAE expression reduced the expression of Snail and Twist in breast cancer cells. We also found that YWHAE was responsible for the resistance of breast cancer cells to chemotherapeutic agents, and knockdown of YWHAE enhanced sensitivity to multiple chemotherapeutic agents in breast cancer cells. Taken together, our findings indicated that YWHAE promoted cancer progression and chemoresistance in breast cancer cells and can be a potential therapeutic target for breast cancer. |
| first_indexed | 2024-12-23T06:06:24Z |
| format | Article |
| id | doaj.art-ac40c156f99e413fa36c3941a61817ac |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-12-23T06:06:24Z |
| publishDate | 2019-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-ac40c156f99e413fa36c3941a61817ac2022-12-21T17:57:32ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502019-07-0135740841610.1002/kjm2.12075YWHAE promotes proliferation, metastasis, and chemoresistance in breast cancer cellsYi‐Fang Yang0Yi‐Chen Lee1Yen‐Yun Wang2Chie‐Hong Wang3Ming‐Feng Hou4Shyng‐Shiou F. Yuan5Translational Research Center Kaohsiung Medical University Hospital Kaohsiung TaiwanDepartment of Anatomy, School of Medicine, College of Medicine Kaohsiung Medical University Kaohsiung TaiwanSchool of Dentistry, College of Dental Medicine Kaohsiung Medical University Kaohsiung TaiwanGraduate Institute of Biomedical Science China Medical University Taichung TaiwanDivision of Breast Surgery, Department of Surgery Kaohsiung Medical University Hospital Kaohsiung TaiwanTranslational Research Center Kaohsiung Medical University Hospital Kaohsiung TaiwanAbstract Breast cancer is the most common female‐specific malignancy in Taiwan and developed countries worldwide, and its incidence continues to grow. 14‐3‐3ε (YWHAE), which belong to 14‐3‐3 family, it has been reported up‐regulated in breast cancer tissues. However, the clinical implication and function of YWHAE in breast cancer remains unclear. In this study, we investigated the prognostic value of the YWHAE in human breast cancer. Immunohistochemistry was used to analyze YWHAE expression in breast cancer tissues. Cell model was applied to examine the functions of YWHAE. The chemotherapeutic agents were used to evaluate the effect of YWHAE in breast cancer cell lines. YWHAE expression was associated with tumor size, lymph node metastasis, and poor patient survival in patients with breast cancer. YWHAE overexpression significantly increased the proliferation, migration, and invasion abilities of breast cancer cells. Knockdown of YWHAE expression reduced the expression of Snail and Twist in breast cancer cells. We also found that YWHAE was responsible for the resistance of breast cancer cells to chemotherapeutic agents, and knockdown of YWHAE enhanced sensitivity to multiple chemotherapeutic agents in breast cancer cells. Taken together, our findings indicated that YWHAE promoted cancer progression and chemoresistance in breast cancer cells and can be a potential therapeutic target for breast cancer.https://doi.org/10.1002/kjm2.1207514‐3‐3εbreast cancerchemoresistanceYWHAE |
| spellingShingle | Yi‐Fang Yang Yi‐Chen Lee Yen‐Yun Wang Chie‐Hong Wang Ming‐Feng Hou Shyng‐Shiou F. Yuan YWHAE promotes proliferation, metastasis, and chemoresistance in breast cancer cells Kaohsiung Journal of Medical Sciences 14‐3‐3ε breast cancer chemoresistance YWHAE |
| title | YWHAE promotes proliferation, metastasis, and chemoresistance in breast cancer cells |
| title_full | YWHAE promotes proliferation, metastasis, and chemoresistance in breast cancer cells |
| title_fullStr | YWHAE promotes proliferation, metastasis, and chemoresistance in breast cancer cells |
| title_full_unstemmed | YWHAE promotes proliferation, metastasis, and chemoresistance in breast cancer cells |
| title_short | YWHAE promotes proliferation, metastasis, and chemoresistance in breast cancer cells |
| title_sort | ywhae promotes proliferation metastasis and chemoresistance in breast cancer cells |
| topic | 14‐3‐3ε breast cancer chemoresistance YWHAE |
| url | https://doi.org/10.1002/kjm2.12075 |
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