Molecular characterization and expression of the feminization-1c (fem-1c) in the freshwater mussel (Hyriopsis cumingii)
The feminization-1c (fem-1c) gene has been shown to be associated with sex differentiation and determination in many metazoan species. It belongs to the fem-1 family which is a member of the ANK superfamily. In this study, the full-length cDNA of the fem-1c (Hcfem-1c) gene was isolated from the fres...
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KeAi Communications Co., Ltd.
2018-01-01
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| Series: | Aquaculture and Fisheries |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468550X1730059X |
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| author | Yayu Wang Congdi Wu Pengfei Guo Guiling Wang Jiale Li |
| author_facet | Yayu Wang Congdi Wu Pengfei Guo Guiling Wang Jiale Li |
| author_sort | Yayu Wang |
| collection | DOAJ |
| description | The feminization-1c (fem-1c) gene has been shown to be associated with sex differentiation and determination in many metazoan species. It belongs to the fem-1 family which is a member of the ANK superfamily. In this study, the full-length cDNA of the fem-1c (Hcfem-1c) gene was isolated from the freshwater mussel (Hypriopsis cumingii). The isolated Hcfem-1c cDNA was 2196 bp in length and encoded a putative protein of 621 amino acids that contains seven ANK domains. Phylogenetic analysis of the deduced HcFEM-1C protein showed that it clustered with the other invertebrates homologues, indicating that the sequence of HcFEM-1C was conserved during evolution. Quantitative real-time PCR (qPCR) expression revealed that the Hcfem-1c gene was expressed in the adductor muscle, foot, liver, gill, kidney, mantle, and gonads of male and female adult mussels (two years old). In the gonads Hcfem-1c was much less abundant in males than that in females. During early development of the gonads, Hcfem-1c transcripts were significantly increased in the primordial germ cell differentiation stage (5 months old). We hypothesized that Hcfem-1c probably regulates female gonad differentiation. In situ hybridization showed that a strong and specific signal concentrated in the female oocyte cell membrane and male follicular wall, indicating that Hcfem-1c gene may not only be involved in female gonad differentiation, but also participates in egg development. This study laid the foundations for a better understanding of gender differentiation mechanism in H. cumingii. |
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| spelling | doaj.art-ac41feb0fe654f7aaf5a6910a291e4a02024-04-16T12:12:16ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2018-01-013161310.1016/j.aaf.2017.10.005Molecular characterization and expression of the feminization-1c (fem-1c) in the freshwater mussel (Hyriopsis cumingii)Yayu Wang0Congdi Wu1Pengfei Guo2Guiling Wang3Jiale Li4Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai, 201306, ChinaKey Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai, 201306, ChinaKey Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai, 201306, ChinaKey Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai, 201306, ChinaKey Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai, 201306, ChinaThe feminization-1c (fem-1c) gene has been shown to be associated with sex differentiation and determination in many metazoan species. It belongs to the fem-1 family which is a member of the ANK superfamily. In this study, the full-length cDNA of the fem-1c (Hcfem-1c) gene was isolated from the freshwater mussel (Hypriopsis cumingii). The isolated Hcfem-1c cDNA was 2196 bp in length and encoded a putative protein of 621 amino acids that contains seven ANK domains. Phylogenetic analysis of the deduced HcFEM-1C protein showed that it clustered with the other invertebrates homologues, indicating that the sequence of HcFEM-1C was conserved during evolution. Quantitative real-time PCR (qPCR) expression revealed that the Hcfem-1c gene was expressed in the adductor muscle, foot, liver, gill, kidney, mantle, and gonads of male and female adult mussels (two years old). In the gonads Hcfem-1c was much less abundant in males than that in females. During early development of the gonads, Hcfem-1c transcripts were significantly increased in the primordial germ cell differentiation stage (5 months old). We hypothesized that Hcfem-1c probably regulates female gonad differentiation. In situ hybridization showed that a strong and specific signal concentrated in the female oocyte cell membrane and male follicular wall, indicating that Hcfem-1c gene may not only be involved in female gonad differentiation, but also participates in egg development. This study laid the foundations for a better understanding of gender differentiation mechanism in H. cumingii.http://www.sciencedirect.com/science/article/pii/S2468550X1730059XHypriopsis cumingiiFem-1cIn situ hybridizationGender differentiation |
| spellingShingle | Yayu Wang Congdi Wu Pengfei Guo Guiling Wang Jiale Li Molecular characterization and expression of the feminization-1c (fem-1c) in the freshwater mussel (Hyriopsis cumingii) Aquaculture and Fisheries Hypriopsis cumingii Fem-1c In situ hybridization Gender differentiation |
| title | Molecular characterization and expression of the feminization-1c (fem-1c) in the freshwater mussel (Hyriopsis cumingii) |
| title_full | Molecular characterization and expression of the feminization-1c (fem-1c) in the freshwater mussel (Hyriopsis cumingii) |
| title_fullStr | Molecular characterization and expression of the feminization-1c (fem-1c) in the freshwater mussel (Hyriopsis cumingii) |
| title_full_unstemmed | Molecular characterization and expression of the feminization-1c (fem-1c) in the freshwater mussel (Hyriopsis cumingii) |
| title_short | Molecular characterization and expression of the feminization-1c (fem-1c) in the freshwater mussel (Hyriopsis cumingii) |
| title_sort | molecular characterization and expression of the feminization 1c fem 1c in the freshwater mussel hyriopsis cumingii |
| topic | Hypriopsis cumingii Fem-1c In situ hybridization Gender differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S2468550X1730059X |
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