Characterization of transcriptome profile and clinical features of a novel immunotherapy target CD204 in diffuse glioma
Abstract CD204 is a specific marker of tumor‐associated macrophages (TAMs) in glioma. However, the expression levels of CD204 and its involvement in glioma are not fully understood. In this large‐scale study, we assessed the expression and function of CD204 in whole‐grade glioma molecularly and clin...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-07-01
|
| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.2312 |
| _version_ | 1811221285955436544 |
|---|---|
| author | Yongliang Yuan Qitai Zhao Songfeng Zhao Penghua Zhang Haibiao Zhao Zeyun Li Yue Du Xin Tian Jingli Lu |
| author_facet | Yongliang Yuan Qitai Zhao Songfeng Zhao Penghua Zhang Haibiao Zhao Zeyun Li Yue Du Xin Tian Jingli Lu |
| author_sort | Yongliang Yuan |
| collection | DOAJ |
| description | Abstract CD204 is a specific marker of tumor‐associated macrophages (TAMs) in glioma. However, the expression levels of CD204 and its involvement in glioma are not fully understood. In this large‐scale study, we assessed the expression and function of CD204 in whole‐grade glioma molecularly and clinically. In total, 1323 glioma samples, including 301 microarray data and 325 RNA‐seq data from the Chinese Glioma Genome Atlas (CGGA) dataset and 697 RNA‐seq data from The Cancer Genome Atlas (TCGA) dataset, were utilized. The statistical analysis and graphical work were mainly performed using the R software. Univariate and multivariate Cox analysis demonstrated that CD204 was an independent prognosticator in glioma patients. CD204 expression was positively correlated with the grade of malignancy. CD204 was consistently upregulated in wild‐type isocitrate dehydrogenase glioma and highly expressed in mesenchymal glioblastoma. Gene ontology of CD204‐related genes showed that CD204 was most enriched in inflammatory response and immune response. It was associated with the stromal and immune populations, especially the monocytic lineage, fibroblasts, and T cells. Circos plots revealed that CD204 was closely associated with many immune checkpoint regulators, especially TIM‐3. CD204 expression is consistent with the malignant phenotype of glioma and independently predicts poor outcomes in glioma patients. Additionally, CD204+ TAMs, collaborating with other checkpoint members, may contribute to the dysfunction of T cells. These findings suggest that CD204 may be a promising target for glioma immunotherapy. |
| first_indexed | 2024-04-12T07:56:44Z |
| format | Article |
| id | doaj.art-ac43616f94cc44c3958a14ccac1c2dac |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-04-12T07:56:44Z |
| publishDate | 2019-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-ac43616f94cc44c3958a14ccac1c2dac2022-12-22T03:41:27ZengWileyCancer Medicine2045-76342019-07-01883811382110.1002/cam4.2312Characterization of transcriptome profile and clinical features of a novel immunotherapy target CD204 in diffuse gliomaYongliang Yuan0Qitai Zhao1Songfeng Zhao2Penghua Zhang3Haibiao Zhao4Zeyun Li5Yue Du6Xin Tian7Jingli Lu8Department of Pharmacy The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaBiotherapy Center The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaDepartment of Pharmacy The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaImaging Department The Third Affiliated Hospital of Zhengzhou University Zhengzhou ChinaDepartment of Neurosurgery The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaDepartment of Pharmacy The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaDepartment of Pharmacy The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaDepartment of Pharmacy The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaDepartment of Pharmacy The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaAbstract CD204 is a specific marker of tumor‐associated macrophages (TAMs) in glioma. However, the expression levels of CD204 and its involvement in glioma are not fully understood. In this large‐scale study, we assessed the expression and function of CD204 in whole‐grade glioma molecularly and clinically. In total, 1323 glioma samples, including 301 microarray data and 325 RNA‐seq data from the Chinese Glioma Genome Atlas (CGGA) dataset and 697 RNA‐seq data from The Cancer Genome Atlas (TCGA) dataset, were utilized. The statistical analysis and graphical work were mainly performed using the R software. Univariate and multivariate Cox analysis demonstrated that CD204 was an independent prognosticator in glioma patients. CD204 expression was positively correlated with the grade of malignancy. CD204 was consistently upregulated in wild‐type isocitrate dehydrogenase glioma and highly expressed in mesenchymal glioblastoma. Gene ontology of CD204‐related genes showed that CD204 was most enriched in inflammatory response and immune response. It was associated with the stromal and immune populations, especially the monocytic lineage, fibroblasts, and T cells. Circos plots revealed that CD204 was closely associated with many immune checkpoint regulators, especially TIM‐3. CD204 expression is consistent with the malignant phenotype of glioma and independently predicts poor outcomes in glioma patients. Additionally, CD204+ TAMs, collaborating with other checkpoint members, may contribute to the dysfunction of T cells. These findings suggest that CD204 may be a promising target for glioma immunotherapy.https://doi.org/10.1002/cam4.2312biomarkerCD204gliomaimmune checkpointsprognosis |
| spellingShingle | Yongliang Yuan Qitai Zhao Songfeng Zhao Penghua Zhang Haibiao Zhao Zeyun Li Yue Du Xin Tian Jingli Lu Characterization of transcriptome profile and clinical features of a novel immunotherapy target CD204 in diffuse glioma Cancer Medicine biomarker CD204 glioma immune checkpoints prognosis |
| title | Characterization of transcriptome profile and clinical features of a novel immunotherapy target CD204 in diffuse glioma |
| title_full | Characterization of transcriptome profile and clinical features of a novel immunotherapy target CD204 in diffuse glioma |
| title_fullStr | Characterization of transcriptome profile and clinical features of a novel immunotherapy target CD204 in diffuse glioma |
| title_full_unstemmed | Characterization of transcriptome profile and clinical features of a novel immunotherapy target CD204 in diffuse glioma |
| title_short | Characterization of transcriptome profile and clinical features of a novel immunotherapy target CD204 in diffuse glioma |
| title_sort | characterization of transcriptome profile and clinical features of a novel immunotherapy target cd204 in diffuse glioma |
| topic | biomarker CD204 glioma immune checkpoints prognosis |
| url | https://doi.org/10.1002/cam4.2312 |
| work_keys_str_mv | AT yongliangyuan characterizationoftranscriptomeprofileandclinicalfeaturesofanovelimmunotherapytargetcd204indiffuseglioma AT qitaizhao characterizationoftranscriptomeprofileandclinicalfeaturesofanovelimmunotherapytargetcd204indiffuseglioma AT songfengzhao characterizationoftranscriptomeprofileandclinicalfeaturesofanovelimmunotherapytargetcd204indiffuseglioma AT penghuazhang characterizationoftranscriptomeprofileandclinicalfeaturesofanovelimmunotherapytargetcd204indiffuseglioma AT haibiaozhao characterizationoftranscriptomeprofileandclinicalfeaturesofanovelimmunotherapytargetcd204indiffuseglioma AT zeyunli characterizationoftranscriptomeprofileandclinicalfeaturesofanovelimmunotherapytargetcd204indiffuseglioma AT yuedu characterizationoftranscriptomeprofileandclinicalfeaturesofanovelimmunotherapytargetcd204indiffuseglioma AT xintian characterizationoftranscriptomeprofileandclinicalfeaturesofanovelimmunotherapytargetcd204indiffuseglioma AT jinglilu characterizationoftranscriptomeprofileandclinicalfeaturesofanovelimmunotherapytargetcd204indiffuseglioma |