Effect of β-caryophyllene from Cloves Extract on <i>Helicobacter pylori</i> Eradication in Mouse Model
New antibacterial treatments against <i>Helicobacter pylori</i> are needed as <i>H. pylori</i> is acquiring antibiotic resistance. β-caryophyllene is a natural bicyclic sesquiterpene, with anti-inflammatory and antimicrobial effects. This study investigates the effects of H-0...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/4/1000 |
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| author | Da Hyun Jung Mi Hee Park Chul Jin Kim Jin Young Lee Chang Yeop Keum In Seon Kim Chang-Hyun Yun Sung-kyu Kim Won Ho Kim Yong Chan Lee |
| author_facet | Da Hyun Jung Mi Hee Park Chul Jin Kim Jin Young Lee Chang Yeop Keum In Seon Kim Chang-Hyun Yun Sung-kyu Kim Won Ho Kim Yong Chan Lee |
| author_sort | Da Hyun Jung |
| collection | DOAJ |
| description | New antibacterial treatments against <i>Helicobacter pylori</i> are needed as <i>H. pylori</i> is acquiring antibiotic resistance. β-caryophyllene is a natural bicyclic sesquiterpene, with anti-inflammatory and antimicrobial effects. This study investigates the effects of H-002119-00-001 from β-caryophyllene on the eradication of <i>H. pylori</i> in a mouse model, and its effects on the inflammation of the gastric mucosa. To evaluate the anti-<i>H.pylori</i> efficacy of β-caryophyllene, a total of 160 mice were divided into eight groups (<i>n</i> = 10 each) and were administered different treatments for 2 and 4 weeks. <i>H. pylori</i> eradication was assessed using a Campylobacter-like organism (CLO) test and <i>H. pylori</i> qPCR of the gastric mucosa. The levels of inflammation of gastric mucosa were assessed using histology and immunostaining. H-002119-00-001 decreased bacterial burden in vitro. When H-002119-00-001 was administered to mice once daily for 2 weeks, cure rates shown by the CLO test were 40.0%, 60.0%, and 70.0% in groups 6, 7, and 8, respectively. <i>H. pylori</i> levels in gastric mucosa decreased dose-dependently after H-002119-00-001 treatment. H-002119-00-001 also reduced levels of inflammation in gastric mucosa. H-002119-00-001 improved inflammation and decreased bacterial burden in <i>H. pylori</i>-infected mouse models. H-002119-00-001 is a promising and effective therapeutic agent for the treatment of <i>H. pylori</i> infection. |
| first_indexed | 2024-03-10T20:40:19Z |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T20:40:19Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-ac4440d4019a40599a301e0163e083862023-11-19T20:43:21ZengMDPI AGNutrients2072-66432020-04-01124100010.3390/nu12041000Effect of β-caryophyllene from Cloves Extract on <i>Helicobacter pylori</i> Eradication in Mouse ModelDa Hyun Jung0Mi Hee Park1Chul Jin Kim2Jin Young Lee3Chang Yeop Keum4In Seon Kim5Chang-Hyun Yun6Sung-kyu Kim7Won Ho Kim8Yong Chan Lee9Department of Internal Medicine, Yonsei University College of Medicine, Seoul 03722, KoreaSFC bio Co., Ltd., 119, Dandae-ro, Dongnam-gu, Cheonan-si, Chungnam 31116, KoreaSFC bio Co., Ltd., 119, Dandae-ro, Dongnam-gu, Cheonan-si, Chungnam 31116, KoreaSFC bio Co., Ltd., 119, Dandae-ro, Dongnam-gu, Cheonan-si, Chungnam 31116, KoreaSFC bio Co., Ltd., 119, Dandae-ro, Dongnam-gu, Cheonan-si, Chungnam 31116, KoreaSFC bio Co., Ltd., 119, Dandae-ro, Dongnam-gu, Cheonan-si, Chungnam 31116, KoreaNew Drug & Bio Research Center, Handok Inc. 2nd floor, A-dong, BioPark, 700, Daewangpangyo-ro, Bundang-gu, Seongnam-si 13488, KoreaSFC bio Co., Ltd., 119, Dandae-ro, Dongnam-gu, Cheonan-si, Chungnam 31116, KoreaDepartment of Internal Medicine, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Internal Medicine, Yonsei University College of Medicine, Seoul 03722, KoreaNew antibacterial treatments against <i>Helicobacter pylori</i> are needed as <i>H. pylori</i> is acquiring antibiotic resistance. β-caryophyllene is a natural bicyclic sesquiterpene, with anti-inflammatory and antimicrobial effects. This study investigates the effects of H-002119-00-001 from β-caryophyllene on the eradication of <i>H. pylori</i> in a mouse model, and its effects on the inflammation of the gastric mucosa. To evaluate the anti-<i>H.pylori</i> efficacy of β-caryophyllene, a total of 160 mice were divided into eight groups (<i>n</i> = 10 each) and were administered different treatments for 2 and 4 weeks. <i>H. pylori</i> eradication was assessed using a Campylobacter-like organism (CLO) test and <i>H. pylori</i> qPCR of the gastric mucosa. The levels of inflammation of gastric mucosa were assessed using histology and immunostaining. H-002119-00-001 decreased bacterial burden in vitro. When H-002119-00-001 was administered to mice once daily for 2 weeks, cure rates shown by the CLO test were 40.0%, 60.0%, and 70.0% in groups 6, 7, and 8, respectively. <i>H. pylori</i> levels in gastric mucosa decreased dose-dependently after H-002119-00-001 treatment. H-002119-00-001 also reduced levels of inflammation in gastric mucosa. H-002119-00-001 improved inflammation and decreased bacterial burden in <i>H. pylori</i>-infected mouse models. H-002119-00-001 is a promising and effective therapeutic agent for the treatment of <i>H. pylori</i> infection.https://www.mdpi.com/2072-6643/12/4/1000β-caryophyllene<i>Helicobacter pylori</i>eradication |
| spellingShingle | Da Hyun Jung Mi Hee Park Chul Jin Kim Jin Young Lee Chang Yeop Keum In Seon Kim Chang-Hyun Yun Sung-kyu Kim Won Ho Kim Yong Chan Lee Effect of β-caryophyllene from Cloves Extract on <i>Helicobacter pylori</i> Eradication in Mouse Model Nutrients β-caryophyllene <i>Helicobacter pylori</i> eradication |
| title | Effect of β-caryophyllene from Cloves Extract on <i>Helicobacter pylori</i> Eradication in Mouse Model |
| title_full | Effect of β-caryophyllene from Cloves Extract on <i>Helicobacter pylori</i> Eradication in Mouse Model |
| title_fullStr | Effect of β-caryophyllene from Cloves Extract on <i>Helicobacter pylori</i> Eradication in Mouse Model |
| title_full_unstemmed | Effect of β-caryophyllene from Cloves Extract on <i>Helicobacter pylori</i> Eradication in Mouse Model |
| title_short | Effect of β-caryophyllene from Cloves Extract on <i>Helicobacter pylori</i> Eradication in Mouse Model |
| title_sort | effect of β caryophyllene from cloves extract on i helicobacter pylori i eradication in mouse model |
| topic | β-caryophyllene <i>Helicobacter pylori</i> eradication |
| url | https://www.mdpi.com/2072-6643/12/4/1000 |
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