Immunoprotection Efficacy of Con A-Purified Proteins against <i>Haemonchus contortus</i> in Goats
Parasitic nematodes are important pathogens that infect animals, causing significant economic losses globally. Current repeated treatments have led to widespread anthelmintic resistance in nematode populations, so vaccine development offers an alternative control approach. However, only one effectiv...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/11/1891 |
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| author | Lisha Ye Yao Zhang Simin Wu Zhiheng Wang Feng Liu Chunqun Wang Min Hu |
| author_facet | Lisha Ye Yao Zhang Simin Wu Zhiheng Wang Feng Liu Chunqun Wang Min Hu |
| author_sort | Lisha Ye |
| collection | DOAJ |
| description | Parasitic nematodes are important pathogens that infect animals, causing significant economic losses globally. Current repeated treatments have led to widespread anthelmintic resistance in nematode populations, so vaccine development offers an alternative control approach. However, only one effective vaccine (named Barbervax) has been developed to protect animals against one of the most pathogenic nematodes of ruminants—<i>Haemonchus contortus</i> (the barber’s pole worm). This vaccine contains a dominant component, Concanavalin A (Con A) purified H11 antigen, which has been shown to induce high levels (>85%) of immune protection in sheep breeds, but in goat breeds, the immunoprotection test of this native protein is still lacking. Here, we evaluated the protective efficacy of low-dose Con A-purified proteins for controlling the <i>H. contortus</i> infection in goats. Four-month-old Boer goats were equally divided into two vaccinated groups of 5 μg and 10 μg native proteins, and one adjuvant control. Each goat was immunized subcutaneously thrice and then challenged with 7000 infective third-stage larvae (L3s). The fecal egg count (FEC), degree of anemia, antibody levels of serum and abomasum mucosa, as well as worm burdens, were detected in experimental goats. Our results showed that both 5 μg and 10 μg vaccinated groups induced the effective protection in goats, reduced mean FEC by 71.8% and 68.6%, and mean worm burdens by 69.8% and 61.6%, respectively, compared to the adjuvant control. In addition, we detected that the serum antibody responses to the Con A-purified proteins were dominated by the IgG subtype, but the mucosal antibody responses were not detected. These data demonstrate Con A-purified proteins induced effective immunoprotection in goats, and underline their significance for controlling this widespread parasite. |
| first_indexed | 2024-03-09T18:34:32Z |
| format | Article |
| id | doaj.art-ac452ecf5bd5418794e60fc7ecfe5a16 |
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| language | English |
| last_indexed | 2024-03-09T18:34:32Z |
| publishDate | 2022-11-01 |
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| series | Vaccines |
| spelling | doaj.art-ac452ecf5bd5418794e60fc7ecfe5a162023-11-24T07:14:20ZengMDPI AGVaccines2076-393X2022-11-011011189110.3390/vaccines10111891Immunoprotection Efficacy of Con A-Purified Proteins against <i>Haemonchus contortus</i> in GoatsLisha Ye0Yao Zhang1Simin Wu2Zhiheng Wang3Feng Liu4Chunqun Wang5Min Hu6State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaParasitic nematodes are important pathogens that infect animals, causing significant economic losses globally. Current repeated treatments have led to widespread anthelmintic resistance in nematode populations, so vaccine development offers an alternative control approach. However, only one effective vaccine (named Barbervax) has been developed to protect animals against one of the most pathogenic nematodes of ruminants—<i>Haemonchus contortus</i> (the barber’s pole worm). This vaccine contains a dominant component, Concanavalin A (Con A) purified H11 antigen, which has been shown to induce high levels (>85%) of immune protection in sheep breeds, but in goat breeds, the immunoprotection test of this native protein is still lacking. Here, we evaluated the protective efficacy of low-dose Con A-purified proteins for controlling the <i>H. contortus</i> infection in goats. Four-month-old Boer goats were equally divided into two vaccinated groups of 5 μg and 10 μg native proteins, and one adjuvant control. Each goat was immunized subcutaneously thrice and then challenged with 7000 infective third-stage larvae (L3s). The fecal egg count (FEC), degree of anemia, antibody levels of serum and abomasum mucosa, as well as worm burdens, were detected in experimental goats. Our results showed that both 5 μg and 10 μg vaccinated groups induced the effective protection in goats, reduced mean FEC by 71.8% and 68.6%, and mean worm burdens by 69.8% and 61.6%, respectively, compared to the adjuvant control. In addition, we detected that the serum antibody responses to the Con A-purified proteins were dominated by the IgG subtype, but the mucosal antibody responses were not detected. These data demonstrate Con A-purified proteins induced effective immunoprotection in goats, and underline their significance for controlling this widespread parasite.https://www.mdpi.com/2076-393X/10/11/1891<i>Haemonchus contortus</i>native proteinimmune protectionfecal egg countantibody response |
| spellingShingle | Lisha Ye Yao Zhang Simin Wu Zhiheng Wang Feng Liu Chunqun Wang Min Hu Immunoprotection Efficacy of Con A-Purified Proteins against <i>Haemonchus contortus</i> in Goats Vaccines <i>Haemonchus contortus</i> native protein immune protection fecal egg count antibody response |
| title | Immunoprotection Efficacy of Con A-Purified Proteins against <i>Haemonchus contortus</i> in Goats |
| title_full | Immunoprotection Efficacy of Con A-Purified Proteins against <i>Haemonchus contortus</i> in Goats |
| title_fullStr | Immunoprotection Efficacy of Con A-Purified Proteins against <i>Haemonchus contortus</i> in Goats |
| title_full_unstemmed | Immunoprotection Efficacy of Con A-Purified Proteins against <i>Haemonchus contortus</i> in Goats |
| title_short | Immunoprotection Efficacy of Con A-Purified Proteins against <i>Haemonchus contortus</i> in Goats |
| title_sort | immunoprotection efficacy of con a purified proteins against i haemonchus contortus i in goats |
| topic | <i>Haemonchus contortus</i> native protein immune protection fecal egg count antibody response |
| url | https://www.mdpi.com/2076-393X/10/11/1891 |
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