Genome-Wide Survey and Functional Verification of the NAC Transcription Factor Family in Wild Emmer Wheat
The NAC transcription factor (TF) family is one of the largest TF families in plants, which has been widely reported in rice, maize and common wheat. However, the significance of the NAC TF family in wild emmer wheat (<i>Triticum turgidum</i> ssp. <i>dicoccoides</i>) is not y...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11598 |
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| author | Fangyi Gong Tian Zhang Zhe Wang Tiangang Qi Yusen Lu Yuhang Liu Shuhong Zhao Ruiqing Liu Rui Yi Jingshu He Bin Tu Tao Zhang Lianquan Zhang Ming Hao Youliang Zheng Dengcai Liu Lin Huang Bihua Wu |
| author_facet | Fangyi Gong Tian Zhang Zhe Wang Tiangang Qi Yusen Lu Yuhang Liu Shuhong Zhao Ruiqing Liu Rui Yi Jingshu He Bin Tu Tao Zhang Lianquan Zhang Ming Hao Youliang Zheng Dengcai Liu Lin Huang Bihua Wu |
| author_sort | Fangyi Gong |
| collection | DOAJ |
| description | The NAC transcription factor (TF) family is one of the largest TF families in plants, which has been widely reported in rice, maize and common wheat. However, the significance of the NAC TF family in wild emmer wheat (<i>Triticum turgidum</i> ssp. <i>dicoccoides</i>) is not yet well understood. In this study, a genome-wide investigation of NAC genes was conducted in the wild emmer genome and 249 NAC family members (<i>TdNAC</i><i>s</i>) were identified. The results showed that all of these genes contained NAM/NAC-conserved domains and most of them were predicted to be located on the nucleus. Phylogenetic analysis showed that these 249 <i>TdNACs</i> can be classified into seven clades, which are likely to be involved in the regulation of grain protein content, starch synthesis and response to biotic and abiotic stresses. Expression pattern analysis revealed that <i>TdNACs</i> were highly expressed in different wheat tissues such as grain, root, leaves and shoots. We found that <i>TdNAC8470</i> was phylogenetically close to NAC genes that regulate either grain protein or starch accumulation. Overexpression of <i>TdNAC8470</i> in rice showed increased grain starch concentration but decreased grain Fe, Zn and Mn contents compared with wild-type plants. Protein interaction analysis indicated that <i>TdNAC8470</i> might interact with granule-bound starch synthase 1 (<i>TdGBSS1</i>) to regulate grain starch accumulation. Our work provides a comprehensive understanding of the NAC TFs family in wild emmer wheat and establishes the way for future functional analysis and genetic improvement of increasing grain starch content in wheat. |
| first_indexed | 2024-03-09T21:39:27Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:39:27Z |
| publishDate | 2022-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ac489544e9844dffadf43823092b883b2023-11-23T20:36:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123191159810.3390/ijms231911598Genome-Wide Survey and Functional Verification of the NAC Transcription Factor Family in Wild Emmer WheatFangyi Gong0Tian Zhang1Zhe Wang2Tiangang Qi3Yusen Lu4Yuhang Liu5Shuhong Zhao6Ruiqing Liu7Rui Yi8Jingshu He9Bin Tu10Tao Zhang11Lianquan Zhang12Ming Hao13Youliang Zheng14Dengcai Liu15Lin Huang16Bihua Wu17State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaRice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaRice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, ChinaThe NAC transcription factor (TF) family is one of the largest TF families in plants, which has been widely reported in rice, maize and common wheat. However, the significance of the NAC TF family in wild emmer wheat (<i>Triticum turgidum</i> ssp. <i>dicoccoides</i>) is not yet well understood. In this study, a genome-wide investigation of NAC genes was conducted in the wild emmer genome and 249 NAC family members (<i>TdNAC</i><i>s</i>) were identified. The results showed that all of these genes contained NAM/NAC-conserved domains and most of them were predicted to be located on the nucleus. Phylogenetic analysis showed that these 249 <i>TdNACs</i> can be classified into seven clades, which are likely to be involved in the regulation of grain protein content, starch synthesis and response to biotic and abiotic stresses. Expression pattern analysis revealed that <i>TdNACs</i> were highly expressed in different wheat tissues such as grain, root, leaves and shoots. We found that <i>TdNAC8470</i> was phylogenetically close to NAC genes that regulate either grain protein or starch accumulation. Overexpression of <i>TdNAC8470</i> in rice showed increased grain starch concentration but decreased grain Fe, Zn and Mn contents compared with wild-type plants. Protein interaction analysis indicated that <i>TdNAC8470</i> might interact with granule-bound starch synthase 1 (<i>TdGBSS1</i>) to regulate grain starch accumulation. Our work provides a comprehensive understanding of the NAC TFs family in wild emmer wheat and establishes the way for future functional analysis and genetic improvement of increasing grain starch content in wheat.https://www.mdpi.com/1422-0067/23/19/11598NAC TF familywild emmer wheattransgenic verificationprotein interaction network |
| spellingShingle | Fangyi Gong Tian Zhang Zhe Wang Tiangang Qi Yusen Lu Yuhang Liu Shuhong Zhao Ruiqing Liu Rui Yi Jingshu He Bin Tu Tao Zhang Lianquan Zhang Ming Hao Youliang Zheng Dengcai Liu Lin Huang Bihua Wu Genome-Wide Survey and Functional Verification of the NAC Transcription Factor Family in Wild Emmer Wheat International Journal of Molecular Sciences NAC TF family wild emmer wheat transgenic verification protein interaction network |
| title | Genome-Wide Survey and Functional Verification of the NAC Transcription Factor Family in Wild Emmer Wheat |
| title_full | Genome-Wide Survey and Functional Verification of the NAC Transcription Factor Family in Wild Emmer Wheat |
| title_fullStr | Genome-Wide Survey and Functional Verification of the NAC Transcription Factor Family in Wild Emmer Wheat |
| title_full_unstemmed | Genome-Wide Survey and Functional Verification of the NAC Transcription Factor Family in Wild Emmer Wheat |
| title_short | Genome-Wide Survey and Functional Verification of the NAC Transcription Factor Family in Wild Emmer Wheat |
| title_sort | genome wide survey and functional verification of the nac transcription factor family in wild emmer wheat |
| topic | NAC TF family wild emmer wheat transgenic verification protein interaction network |
| url | https://www.mdpi.com/1422-0067/23/19/11598 |
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