Wild primate microbiomes prevent weight gain in germ-free mice
Abstract Background The gut microbiome harbors trillions of bacteria that play a major role in dietary nutrient extraction and host metabolism. Metabolic diseases such as obesity and diabetes are associated with shifts in microbiome composition and have been on the rise in Westernized or highly indu...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-05-01
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| Series: | Animal Microbiome |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s42523-020-00033-9 |
| _version_ | 1811323592288239616 |
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| author | Dimitrios N. Sidiropoulos Gabriel A. Al-Ghalith Robin R. Shields-Cutler Tonya L. Ward Abigail J. Johnson Pajau Vangay Dan Knights Purna C. Kashyap Yibo Xian Amanda E. Ramer-Tait Jonathan B. Clayton |
| author_facet | Dimitrios N. Sidiropoulos Gabriel A. Al-Ghalith Robin R. Shields-Cutler Tonya L. Ward Abigail J. Johnson Pajau Vangay Dan Knights Purna C. Kashyap Yibo Xian Amanda E. Ramer-Tait Jonathan B. Clayton |
| author_sort | Dimitrios N. Sidiropoulos |
| collection | DOAJ |
| description | Abstract Background The gut microbiome harbors trillions of bacteria that play a major role in dietary nutrient extraction and host metabolism. Metabolic diseases such as obesity and diabetes are associated with shifts in microbiome composition and have been on the rise in Westernized or highly industrialized countries. At the same time, Westernized diets low in dietary fiber have been shown to cause loss of gut microbial diversity. However, the link between microbiome composition, loss of dietary fiber, and obesity has not been well defined. Results To study the interactions between gut microbiota, dietary fiber, and weight gain, we transplanted captive and wild douc gut microbiota into germ-free mice and then exposed them to either a high- or low-fiber diet. The group receiving captive douc microbiota gained significantly more weight, regardless of diet, while mice receiving a high-fiber diet and wild douc microbiota remained lean. In the presence of a low-fiber diet, the wild douc microbiota partially prevented weight gain. Using 16S rRNA gene amplicon sequencing we identified key bacterial taxa in each group, specifically a high relative abundance of Bacteroides and Akkermansia in captive douc FMT mice and a higher relative abundance of Lactobacillus and Clostridium in the wild douc FMT mice. Conclusions In the context of our germ-free mouse experiment, wild douc microbiota could serve as a reservoir for microbes for cross-species transplants. Our results suggest that wild douc microbiota are tailored to diverse fiber diets and can prevent weight gain when exposed to a native diet. Graphical abstract |
| first_indexed | 2024-04-13T13:58:54Z |
| format | Article |
| id | doaj.art-ac4915b761424c8ab9f91ae050147814 |
| institution | Directory Open Access Journal |
| issn | 2524-4671 |
| language | English |
| last_indexed | 2024-04-13T13:58:54Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Animal Microbiome |
| spelling | doaj.art-ac4915b761424c8ab9f91ae0501478142022-12-22T02:44:07ZengBMCAnimal Microbiome2524-46712020-05-012111110.1186/s42523-020-00033-9Wild primate microbiomes prevent weight gain in germ-free miceDimitrios N. Sidiropoulos0Gabriel A. Al-Ghalith1Robin R. Shields-Cutler2Tonya L. Ward3Abigail J. Johnson4Pajau Vangay5Dan Knights6Purna C. Kashyap7Yibo Xian8Amanda E. Ramer-Tait9Jonathan B. Clayton10Biotechnology Institute, University of MinnesotaBiotechnology Institute, University of MinnesotaBiotechnology Institute, University of MinnesotaBiotechnology Institute, University of MinnesotaBiotechnology Institute, University of MinnesotaBiotechnology Institute, University of MinnesotaBiotechnology Institute, University of MinnesotaEnteric Neuroscience Program, Division of Gastroenterology & Hepatology, Departments of Medicine and Physiology & Biomedical Engineering, Mayo ClinicDepartment of Food Science and Technology, University of Nebraska-LincolnDepartment of Food Science and Technology, University of Nebraska-LincolnBiotechnology Institute, University of MinnesotaAbstract Background The gut microbiome harbors trillions of bacteria that play a major role in dietary nutrient extraction and host metabolism. Metabolic diseases such as obesity and diabetes are associated with shifts in microbiome composition and have been on the rise in Westernized or highly industrialized countries. At the same time, Westernized diets low in dietary fiber have been shown to cause loss of gut microbial diversity. However, the link between microbiome composition, loss of dietary fiber, and obesity has not been well defined. Results To study the interactions between gut microbiota, dietary fiber, and weight gain, we transplanted captive and wild douc gut microbiota into germ-free mice and then exposed them to either a high- or low-fiber diet. The group receiving captive douc microbiota gained significantly more weight, regardless of diet, while mice receiving a high-fiber diet and wild douc microbiota remained lean. In the presence of a low-fiber diet, the wild douc microbiota partially prevented weight gain. Using 16S rRNA gene amplicon sequencing we identified key bacterial taxa in each group, specifically a high relative abundance of Bacteroides and Akkermansia in captive douc FMT mice and a higher relative abundance of Lactobacillus and Clostridium in the wild douc FMT mice. Conclusions In the context of our germ-free mouse experiment, wild douc microbiota could serve as a reservoir for microbes for cross-species transplants. Our results suggest that wild douc microbiota are tailored to diverse fiber diets and can prevent weight gain when exposed to a native diet. Graphical abstracthttp://link.springer.com/article/10.1186/s42523-020-00033-9MicrobiomeNonhuman primateRed-shanked doucDysbiosisWesternizationFecal microbiota transplantation |
| spellingShingle | Dimitrios N. Sidiropoulos Gabriel A. Al-Ghalith Robin R. Shields-Cutler Tonya L. Ward Abigail J. Johnson Pajau Vangay Dan Knights Purna C. Kashyap Yibo Xian Amanda E. Ramer-Tait Jonathan B. Clayton Wild primate microbiomes prevent weight gain in germ-free mice Animal Microbiome Microbiome Nonhuman primate Red-shanked douc Dysbiosis Westernization Fecal microbiota transplantation |
| title | Wild primate microbiomes prevent weight gain in germ-free mice |
| title_full | Wild primate microbiomes prevent weight gain in germ-free mice |
| title_fullStr | Wild primate microbiomes prevent weight gain in germ-free mice |
| title_full_unstemmed | Wild primate microbiomes prevent weight gain in germ-free mice |
| title_short | Wild primate microbiomes prevent weight gain in germ-free mice |
| title_sort | wild primate microbiomes prevent weight gain in germ free mice |
| topic | Microbiome Nonhuman primate Red-shanked douc Dysbiosis Westernization Fecal microbiota transplantation |
| url | http://link.springer.com/article/10.1186/s42523-020-00033-9 |
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