Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells
The origin of Langerhans cells (LCs), which are skin epidermis-resident macrophages, remains unclear. Current lineage tracing of LCs largely relies on the promoter-Cre-LoxP system, which often gives rise to contradictory conclusions with different promoters. Thus, reinvestigation with an improved tr...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-06-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/36131 |
| _version_ | 1811235992310382592 |
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| author | Sicong He Jiahao Chen Yunyun Jiang Yi Wu Lu Zhu Wan Jin Changlong Zhao Tao Yu Tienan Wang Shuting Wu Xi Lin Jianan Y Qu Zilong Wen Wenqing Zhang Jin Xu |
| author_facet | Sicong He Jiahao Chen Yunyun Jiang Yi Wu Lu Zhu Wan Jin Changlong Zhao Tao Yu Tienan Wang Shuting Wu Xi Lin Jianan Y Qu Zilong Wen Wenqing Zhang Jin Xu |
| author_sort | Sicong He |
| collection | DOAJ |
| description | The origin of Langerhans cells (LCs), which are skin epidermis-resident macrophages, remains unclear. Current lineage tracing of LCs largely relies on the promoter-Cre-LoxP system, which often gives rise to contradictory conclusions with different promoters. Thus, reinvestigation with an improved tracing method is necessary. Here, using a laser-mediated temporal-spatial resolved cell labeling method, we demonstrated that most adult LCs originated from the ventral wall of the dorsal aorta (VDA), an equivalent to the mouse aorta, gonads, and mesonephros (AGM), where both hematopoietic stem cells (HSCs) and non-HSC progenitors are generated. Further fine-fate mapping analysis revealed that the appearance of LCs in adult zebrafish was correlated with the development of HSCs, but not T cell progenitors. Finally, we showed that the appearance of tissue-resident macrophages in the brain, liver, heart, and gut of adult zebrafish was also correlated with HSCs. Thus, the results of our study challenged the EMP-origin theory for LCs. |
| first_indexed | 2024-04-12T12:01:38Z |
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| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:01:38Z |
| publishDate | 2018-06-01 |
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| record_format | Article |
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| spelling | doaj.art-ac49cf7168d24bcdbdd56311a7a742402022-12-22T03:33:51ZengeLife Sciences Publications LtdeLife2050-084X2018-06-01710.7554/eLife.36131Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cellsSicong He0https://orcid.org/0000-0002-0399-3904Jiahao Chen1Yunyun Jiang2Yi Wu3Lu Zhu4Wan Jin5Changlong Zhao6Tao Yu7Tienan Wang8Shuting Wu9Xi Lin10Jianan Y Qu11Zilong Wen12Wenqing Zhang13Jin Xu14https://orcid.org/0000-0002-6840-1359Department of Electronic and Computer Engineering, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Hong Kong, ChinaKey Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaKey Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaDivision of Life Science and State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Hong Kong, ChinaDivision of Life Science and State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Hong Kong, ChinaDivision of Life Science and State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Hong Kong, ChinaDivision of Life Science and State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Hong Kong, ChinaShenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University, The Hong Kong University of Science and Technology Medical Center, Shenzhen, ChinaDivision of Life Science and State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Hong Kong, ChinaDivision of Life Science and State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Hong Kong, ChinaDivision of Life Science and State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Hong Kong, ChinaDepartment of Electronic and Computer Engineering, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Hong Kong, ChinaDivision of Life Science and State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Hong Kong, ChinaKey Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China; Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology, Guangzhou, ChinaDivision of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology, Guangzhou, ChinaThe origin of Langerhans cells (LCs), which are skin epidermis-resident macrophages, remains unclear. Current lineage tracing of LCs largely relies on the promoter-Cre-LoxP system, which often gives rise to contradictory conclusions with different promoters. Thus, reinvestigation with an improved tracing method is necessary. Here, using a laser-mediated temporal-spatial resolved cell labeling method, we demonstrated that most adult LCs originated from the ventral wall of the dorsal aorta (VDA), an equivalent to the mouse aorta, gonads, and mesonephros (AGM), where both hematopoietic stem cells (HSCs) and non-HSC progenitors are generated. Further fine-fate mapping analysis revealed that the appearance of LCs in adult zebrafish was correlated with the development of HSCs, but not T cell progenitors. Finally, we showed that the appearance of tissue-resident macrophages in the brain, liver, heart, and gut of adult zebrafish was also correlated with HSCs. Thus, the results of our study challenged the EMP-origin theory for LCs.https://elifesciences.org/articles/36131Langerhans celltissue resident macrophagelineage tracingzebrafish |
| spellingShingle | Sicong He Jiahao Chen Yunyun Jiang Yi Wu Lu Zhu Wan Jin Changlong Zhao Tao Yu Tienan Wang Shuting Wu Xi Lin Jianan Y Qu Zilong Wen Wenqing Zhang Jin Xu Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells eLife Langerhans cell tissue resident macrophage lineage tracing zebrafish |
| title | Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells |
| title_full | Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells |
| title_fullStr | Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells |
| title_full_unstemmed | Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells |
| title_short | Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells |
| title_sort | adult zebrafish langerhans cells arise from hematopoietic stem progenitor cells |
| topic | Langerhans cell tissue resident macrophage lineage tracing zebrafish |
| url | https://elifesciences.org/articles/36131 |
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