Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex
Chromatin boundaries subdivide eukaryotic chromosomes into functionally autonomous domains of genetic activity. This subdivision insulates genes and/or regulatory elements within a domain from promiscuous interactions with nearby domains. While it was previously assumed that the chromosomal domain l...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2012-12-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/00171 |
| _version_ | 1811201495399399424 |
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| author | Tsutomu Aoki Ali Sarkeshik John Yates Paul Schedl |
| author_facet | Tsutomu Aoki Ali Sarkeshik John Yates Paul Schedl |
| author_sort | Tsutomu Aoki |
| collection | DOAJ |
| description | Chromatin boundaries subdivide eukaryotic chromosomes into functionally autonomous domains of genetic activity. This subdivision insulates genes and/or regulatory elements within a domain from promiscuous interactions with nearby domains. While it was previously assumed that the chromosomal domain landscape is fixed, there is now growing evidence that the landscape may be subject to tissue and stage specific regulation. Here we report the isolation and characterization of a novel developmentally restricted boundary factor, Elba. We show that Elba is an unusual hetero-tripartite protein complex that requires all three proteins for DNA binding and insulator activity. |
| first_indexed | 2024-04-12T02:22:36Z |
| format | Article |
| id | doaj.art-ac4ee55961644bb487ebd502bcd03293 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:22:36Z |
| publishDate | 2012-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-ac4ee55961644bb487ebd502bcd032932022-12-22T03:52:05ZengeLife Sciences Publications LtdeLife2050-084X2012-12-01110.7554/eLife.00171Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complexTsutomu Aoki0Ali Sarkeshik1John Yates2Paul Schedl3Department of Molecular Biology, Princeton University, Princeton, United StatesDepartment of Chemical Physiology, Scripps Research Institute, La Jolla, United StatesDepartment of Chemical Physiology, Scripps Research Institute, La Jolla, United StatesDepartment of Molecular Biology, Princeton University, Princeton, United StatesChromatin boundaries subdivide eukaryotic chromosomes into functionally autonomous domains of genetic activity. This subdivision insulates genes and/or regulatory elements within a domain from promiscuous interactions with nearby domains. While it was previously assumed that the chromosomal domain landscape is fixed, there is now growing evidence that the landscape may be subject to tissue and stage specific regulation. Here we report the isolation and characterization of a novel developmentally restricted boundary factor, Elba. We show that Elba is an unusual hetero-tripartite protein complex that requires all three proteins for DNA binding and insulator activity.https://elifesciences.org/articles/00171boundaryInsulatorDomainChromatinBithoraxDevelopment |
| spellingShingle | Tsutomu Aoki Ali Sarkeshik John Yates Paul Schedl Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex eLife boundary Insulator Domain Chromatin Bithorax Development |
| title | Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex |
| title_full | Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex |
| title_fullStr | Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex |
| title_full_unstemmed | Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex |
| title_short | Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex |
| title_sort | elba a novel developmentally regulated chromatin boundary factor is a hetero tripartite dna binding complex |
| topic | boundary Insulator Domain Chromatin Bithorax Development |
| url | https://elifesciences.org/articles/00171 |
| work_keys_str_mv | AT tsutomuaoki elbaanoveldevelopmentallyregulatedchromatinboundaryfactorisaheterotripartitednabindingcomplex AT alisarkeshik elbaanoveldevelopmentallyregulatedchromatinboundaryfactorisaheterotripartitednabindingcomplex AT johnyates elbaanoveldevelopmentallyregulatedchromatinboundaryfactorisaheterotripartitednabindingcomplex AT paulschedl elbaanoveldevelopmentallyregulatedchromatinboundaryfactorisaheterotripartitednabindingcomplex |