Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by <sup>1</sup>H-NMR
In the present study, data related to the metabolomics of saliva and serum in trained standardbred horses are provided for the first time. Metabolomic analysis allows to analyze all the metabolites within selected biofluids, providing a better understanding of biochemistry modifications related to e...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/10/7/298 |
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| author | Marilena Bazzano Luca Laghi Chenglin Zhu Enrica Lotito Stefano Sgariglia Beniamino Tesei Fulvio Laus |
| author_facet | Marilena Bazzano Luca Laghi Chenglin Zhu Enrica Lotito Stefano Sgariglia Beniamino Tesei Fulvio Laus |
| author_sort | Marilena Bazzano |
| collection | DOAJ |
| description | In the present study, data related to the metabolomics of saliva and serum in trained standardbred horses are provided for the first time. Metabolomic analysis allows to analyze all the metabolites within selected biofluids, providing a better understanding of biochemistry modifications related to exercise. On the basis of the current advances observed in metabolomic research on human athletes, we aimed to investigate the metabolites’ profile of serum and saliva samples collected from healthy standardbred horses and the relationship with physical exercise. Twelve trained standardbred horses were sampled for blood and saliva before (T<sub>0</sub>) and immediately after (T<sub>1</sub>) standardized exercise. Metabolomic analysis of both samples was performed by <sup>1</sup>H-NMR spectroscopy. Forty-six metabolites in serum and 62 metabolites in saliva were detected, including alcohols, amino acids, organic acids, carbohydrates and purine derivatives. Twenty-six and 14 metabolites resulted to be significantly changed between T<sub>0</sub> and T<sub>1</sub> in serum and saliva, respectively. The findings of 2-hydroxyisobutyrate and 3-hydroxybutyrate in serum and GABA in equine saliva, as well as their modifications following exercise, provide new insights about the physiology of exercise in athletic horses. Glycerol might represent a novel biomarker for fitness evaluation in sport horses. |
| first_indexed | 2024-03-10T18:19:07Z |
| format | Article |
| id | doaj.art-ac5187366fe64faf89fe00469872cbdd |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T18:19:07Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-ac5187366fe64faf89fe00469872cbdd2023-11-20T07:27:17ZengMDPI AGMetabolites2218-19892020-07-0110729810.3390/metabo10070298Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by <sup>1</sup>H-NMRMarilena Bazzano0Luca Laghi1Chenglin Zhu2Enrica Lotito3Stefano Sgariglia4Beniamino Tesei5Fulvio Laus6School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione, 93/95, 62024 Matelica, ItalyDepartment of Agricultural and Food Sciences, University of Bologna, 47521 Cesena, ItalyDepartment of Agricultural and Food Sciences, University of Bologna, 47521 Cesena, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione, 93/95, 62024 Matelica, ItalyPractitioner, 63900 Fermo, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione, 93/95, 62024 Matelica, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione, 93/95, 62024 Matelica, ItalyIn the present study, data related to the metabolomics of saliva and serum in trained standardbred horses are provided for the first time. Metabolomic analysis allows to analyze all the metabolites within selected biofluids, providing a better understanding of biochemistry modifications related to exercise. On the basis of the current advances observed in metabolomic research on human athletes, we aimed to investigate the metabolites’ profile of serum and saliva samples collected from healthy standardbred horses and the relationship with physical exercise. Twelve trained standardbred horses were sampled for blood and saliva before (T<sub>0</sub>) and immediately after (T<sub>1</sub>) standardized exercise. Metabolomic analysis of both samples was performed by <sup>1</sup>H-NMR spectroscopy. Forty-six metabolites in serum and 62 metabolites in saliva were detected, including alcohols, amino acids, organic acids, carbohydrates and purine derivatives. Twenty-six and 14 metabolites resulted to be significantly changed between T<sub>0</sub> and T<sub>1</sub> in serum and saliva, respectively. The findings of 2-hydroxyisobutyrate and 3-hydroxybutyrate in serum and GABA in equine saliva, as well as their modifications following exercise, provide new insights about the physiology of exercise in athletic horses. Glycerol might represent a novel biomarker for fitness evaluation in sport horses.https://www.mdpi.com/2218-1989/10/7/298horsemetabolomicmetabolismexercisesaliva |
| spellingShingle | Marilena Bazzano Luca Laghi Chenglin Zhu Enrica Lotito Stefano Sgariglia Beniamino Tesei Fulvio Laus Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by <sup>1</sup>H-NMR Metabolites horse metabolomic metabolism exercise saliva |
| title | Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by <sup>1</sup>H-NMR |
| title_full | Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by <sup>1</sup>H-NMR |
| title_fullStr | Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by <sup>1</sup>H-NMR |
| title_full_unstemmed | Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by <sup>1</sup>H-NMR |
| title_short | Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by <sup>1</sup>H-NMR |
| title_sort | exercise induced changes in salivary and serum metabolome in trained standardbred assessed by sup 1 sup h nmr |
| topic | horse metabolomic metabolism exercise saliva |
| url | https://www.mdpi.com/2218-1989/10/7/298 |
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