Amphotropic murine leukaemia virus envelope protein is associated with cholesterol-rich microdomains

<p>Abstract</p> <p>Background</p> <p>Cholesterol-rich microdomains like lipid rafts were recently identified as regions within the plasma membrane, which play an important role in the assembly and budding of different viruses, e.g., measles virus and human immunodeficie...

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Main Authors: Pedersen Lene, Beer Christiane, Wirth Manfred
Format: Article
Language:English
Published: BMC 2005-04-01
Series:Virology Journal
Online Access:http://www.virologyj.com/content/2/1/36
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author Pedersen Lene
Beer Christiane
Wirth Manfred
author_facet Pedersen Lene
Beer Christiane
Wirth Manfred
author_sort Pedersen Lene
collection DOAJ
description <p>Abstract</p> <p>Background</p> <p>Cholesterol-rich microdomains like lipid rafts were recently identified as regions within the plasma membrane, which play an important role in the assembly and budding of different viruses, e.g., measles virus and human immunodeficiency virus. For these viruses association of newly synthesized viral proteins with lipid rafts has been shown.</p> <p>Results</p> <p>Here we provide evidence for the association of the envelope protein (Env) of the 4070A isolate of amphotropic murine leukaemia virus (A-MLV) with lipid rafts. Using density gradient centrifugation and immunocytochemical analyses, we show that Env co-localizes with cholesterol, ganglioside GM1 and caveolin-1 in these specific regions of the plasma membrane.</p> <p>Conclusions</p> <p>These results show that a large amount of A-MLV Env is associated with lipid rafts and suggest that cholesterol-rich microdomains are used as portals for the exit of A-MLV.</p>
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spelling doaj.art-ac520520b0bc4452a2e0706fea6ab3b72022-12-21T20:59:25ZengBMCVirology Journal1743-422X2005-04-01213610.1186/1743-422X-2-36Amphotropic murine leukaemia virus envelope protein is associated with cholesterol-rich microdomainsPedersen LeneBeer ChristianeWirth Manfred<p>Abstract</p> <p>Background</p> <p>Cholesterol-rich microdomains like lipid rafts were recently identified as regions within the plasma membrane, which play an important role in the assembly and budding of different viruses, e.g., measles virus and human immunodeficiency virus. For these viruses association of newly synthesized viral proteins with lipid rafts has been shown.</p> <p>Results</p> <p>Here we provide evidence for the association of the envelope protein (Env) of the 4070A isolate of amphotropic murine leukaemia virus (A-MLV) with lipid rafts. Using density gradient centrifugation and immunocytochemical analyses, we show that Env co-localizes with cholesterol, ganglioside GM1 and caveolin-1 in these specific regions of the plasma membrane.</p> <p>Conclusions</p> <p>These results show that a large amount of A-MLV Env is associated with lipid rafts and suggest that cholesterol-rich microdomains are used as portals for the exit of A-MLV.</p>http://www.virologyj.com/content/2/1/36
spellingShingle Pedersen Lene
Beer Christiane
Wirth Manfred
Amphotropic murine leukaemia virus envelope protein is associated with cholesterol-rich microdomains
Virology Journal
title Amphotropic murine leukaemia virus envelope protein is associated with cholesterol-rich microdomains
title_full Amphotropic murine leukaemia virus envelope protein is associated with cholesterol-rich microdomains
title_fullStr Amphotropic murine leukaemia virus envelope protein is associated with cholesterol-rich microdomains
title_full_unstemmed Amphotropic murine leukaemia virus envelope protein is associated with cholesterol-rich microdomains
title_short Amphotropic murine leukaemia virus envelope protein is associated with cholesterol-rich microdomains
title_sort amphotropic murine leukaemia virus envelope protein is associated with cholesterol rich microdomains
url http://www.virologyj.com/content/2/1/36
work_keys_str_mv AT pedersenlene amphotropicmurineleukaemiavirusenvelopeproteinisassociatedwithcholesterolrichmicrodomains
AT beerchristiane amphotropicmurineleukaemiavirusenvelopeproteinisassociatedwithcholesterolrichmicrodomains
AT wirthmanfred amphotropicmurineleukaemiavirusenvelopeproteinisassociatedwithcholesterolrichmicrodomains