Emerging Roles of Heat-Induced circRNAs Related to Lactogenesis in Lactating Sows
Heat stress negatively influences milk production and disrupts normal physiological activity of lactating sows, but the precious mechanisms by which hyperthermia adversely affects milk synthesis in sows still remain for further study. Circular RNAs are a novel class of non-coding RNAs with regulator...
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2019.01347/full |
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| author | Jiajie Sun Haojie Zhang Baoyu Hu Yueqin Xie Dongyang Wang Jinzhi Zhang Ting Chen Junyi Luo Songbo Wang Qinyan Jiang Qianyun Xi Zujing Chen Yongliang Zhang |
| author_facet | Jiajie Sun Haojie Zhang Baoyu Hu Yueqin Xie Dongyang Wang Jinzhi Zhang Ting Chen Junyi Luo Songbo Wang Qinyan Jiang Qianyun Xi Zujing Chen Yongliang Zhang |
| author_sort | Jiajie Sun |
| collection | DOAJ |
| description | Heat stress negatively influences milk production and disrupts normal physiological activity of lactating sows, but the precious mechanisms by which hyperthermia adversely affects milk synthesis in sows still remain for further study. Circular RNAs are a novel class of non-coding RNAs with regulatory functions in various physiological and pathological processes. The expression profiles and functions of circRNAs of sows in lactogenesis remain largely unknown. In the present study, long-term heat stress (HS) resulted in a greater concentration of serum HSP70, LDH, and IgG, as well as decreased levels of COR, SOD, and PRL. HS reduced the total solids, fat, and lactose of sow milk, and HS significantly depressed CSNαs1, CSNαs2, and CSNκ biosynthesis. Transcriptome sequencing of lactating porcine mammary glands identified 42 upregulated and 25 downregulated transcripts in HS vs. control. Functional annotation of these differentially-expressed transcripts revealed four heat-induced genes involved in lactation. Moreover, 29 upregulated and 21 downregulated circRNA candidates were found in response to HS. Forty-two positively correlated circRNA-mRNA expression patterns were constructed between the four lactogenic genes and differentially expressed circRNAs. Five circRNA-miRNA-mRNA post-transcriptional networks were identified involving genes in the HS response of lactating sows. In this study we establish a valuable resource for circRNA biology in sow lactation. Analysis of a circRNA-miRNA-mRNA network further uncovered a novel layer of post-transcriptional regulation that could be used to improve sow milk production. |
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| language | English |
| last_indexed | 2024-12-22T19:18:43Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-ac521e2972c24b6eae02df7c3c32f9a02022-12-21T18:15:26ZengFrontiers Media S.A.Frontiers in Genetics1664-80212020-02-011010.3389/fgene.2019.01347475110Emerging Roles of Heat-Induced circRNAs Related to Lactogenesis in Lactating SowsJiajie Sun0Haojie Zhang1Baoyu Hu2Yueqin Xie3Dongyang Wang4Jinzhi Zhang5Ting Chen6Junyi Luo7Songbo Wang8Qinyan Jiang9Qianyun Xi10Zujing Chen11Yongliang Zhang12College of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Engineering & Research Center for Woody Fodder Plants, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Engineering & Research Center for Woody Fodder Plants, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Engineering & Research Center for Woody Fodder Plants, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Engineering & Research Center for Woody Fodder Plants, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Engineering & Research Center for Woody Fodder Plants, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, Zhejiang University, Hangzhou, ChinaCollege of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Engineering & Research Center for Woody Fodder Plants, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Engineering & Research Center for Woody Fodder Plants, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Engineering & Research Center for Woody Fodder Plants, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Engineering & Research Center for Woody Fodder Plants, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Engineering & Research Center for Woody Fodder Plants, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Engineering & Research Center for Woody Fodder Plants, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Engineering & Research Center for Woody Fodder Plants, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, ChinaHeat stress negatively influences milk production and disrupts normal physiological activity of lactating sows, but the precious mechanisms by which hyperthermia adversely affects milk synthesis in sows still remain for further study. Circular RNAs are a novel class of non-coding RNAs with regulatory functions in various physiological and pathological processes. The expression profiles and functions of circRNAs of sows in lactogenesis remain largely unknown. In the present study, long-term heat stress (HS) resulted in a greater concentration of serum HSP70, LDH, and IgG, as well as decreased levels of COR, SOD, and PRL. HS reduced the total solids, fat, and lactose of sow milk, and HS significantly depressed CSNαs1, CSNαs2, and CSNκ biosynthesis. Transcriptome sequencing of lactating porcine mammary glands identified 42 upregulated and 25 downregulated transcripts in HS vs. control. Functional annotation of these differentially-expressed transcripts revealed four heat-induced genes involved in lactation. Moreover, 29 upregulated and 21 downregulated circRNA candidates were found in response to HS. Forty-two positively correlated circRNA-mRNA expression patterns were constructed between the four lactogenic genes and differentially expressed circRNAs. Five circRNA-miRNA-mRNA post-transcriptional networks were identified involving genes in the HS response of lactating sows. In this study we establish a valuable resource for circRNA biology in sow lactation. Analysis of a circRNA-miRNA-mRNA network further uncovered a novel layer of post-transcriptional regulation that could be used to improve sow milk production.https://www.frontiersin.org/article/10.3389/fgene.2019.01347/fullheat stresslactating sowcircRNAceRNAcasein |
| spellingShingle | Jiajie Sun Haojie Zhang Baoyu Hu Yueqin Xie Dongyang Wang Jinzhi Zhang Ting Chen Junyi Luo Songbo Wang Qinyan Jiang Qianyun Xi Zujing Chen Yongliang Zhang Emerging Roles of Heat-Induced circRNAs Related to Lactogenesis in Lactating Sows Frontiers in Genetics heat stress lactating sow circRNA ceRNA casein |
| title | Emerging Roles of Heat-Induced circRNAs Related to Lactogenesis in Lactating Sows |
| title_full | Emerging Roles of Heat-Induced circRNAs Related to Lactogenesis in Lactating Sows |
| title_fullStr | Emerging Roles of Heat-Induced circRNAs Related to Lactogenesis in Lactating Sows |
| title_full_unstemmed | Emerging Roles of Heat-Induced circRNAs Related to Lactogenesis in Lactating Sows |
| title_short | Emerging Roles of Heat-Induced circRNAs Related to Lactogenesis in Lactating Sows |
| title_sort | emerging roles of heat induced circrnas related to lactogenesis in lactating sows |
| topic | heat stress lactating sow circRNA ceRNA casein |
| url | https://www.frontiersin.org/article/10.3389/fgene.2019.01347/full |
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