A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs
Abstract The requirement for immediate vascularization of engineered dental pulp poses a major hurdle towards successful implementation of pulp regeneration as an effective therapeutic strategy for root canal therapy, especially in adult teeth. Here, we demonstrate a novel strategy to engineer pre-v...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-02532-3 |
| _version_ | 1819042832979066880 |
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| author | Avathamsa Athirasala Fernanda Lins Anthony Tahayeri Monica Hinds Anthony J. Smith Christine Sedgley Jack Ferracane Luiz E. Bertassoni |
| author_facet | Avathamsa Athirasala Fernanda Lins Anthony Tahayeri Monica Hinds Anthony J. Smith Christine Sedgley Jack Ferracane Luiz E. Bertassoni |
| author_sort | Avathamsa Athirasala |
| collection | DOAJ |
| description | Abstract The requirement for immediate vascularization of engineered dental pulp poses a major hurdle towards successful implementation of pulp regeneration as an effective therapeutic strategy for root canal therapy, especially in adult teeth. Here, we demonstrate a novel strategy to engineer pre-vascularized, cell-laden hydrogel pulp-like tissue constructs in full-length root canals for dental pulp regeneration. We utilized gelatin methacryloyl (GelMA) hydrogels with tunable physical and mechanical properties to determine the microenvironmental conditions (microstructure, degradation, swelling and elastic modulus) that enhanced viability, spreading and proliferation of encapsulated odontoblast-like cells (OD21), and the formation of endothelial monolayers by endothelial colony forming cells (ECFCs). GelMA hydrogels with higher polymer concentration (15% w/v) and stiffness enhanced OD21 cell viability, spreading and proliferation, as well as endothelial cell spreading and monolayer formation. We then fabricated pre-vascularized, full-length, dental pulp-like tissue constructs by dispensing OD21 cell-laden GelMA hydrogel prepolymer in root canals of extracted teeth and fabricating 500 µm channels throughout the root canals. ECFCs seeded into the microchannels successfully formed monolayers and underwent angiogenic sprouting within 7 days in culture. In summary, the proposed approach is a simple and effective strategy for engineering of pre-vascularized dental pulp constructs offering potentially beneficial translational outcomes. |
| first_indexed | 2024-12-21T09:47:10Z |
| format | Article |
| id | doaj.art-ac55dfd1867d4e76a388a1b269be79c7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T09:47:10Z |
| publishDate | 2017-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ac55dfd1867d4e76a388a1b269be79c72022-12-21T19:08:18ZengNature PortfolioScientific Reports2045-23222017-06-017111110.1038/s41598-017-02532-3A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue ConstructsAvathamsa Athirasala0Fernanda Lins1Anthony Tahayeri2Monica Hinds3Anthony J. Smith4Christine Sedgley5Jack Ferracane6Luiz E. Bertassoni7Division of Biomaterials and Biomechanics, Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science UniversityDivision of Biomaterials and Biomechanics, Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science UniversityDivision of Biomaterials and Biomechanics, Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science UniversityDepartment of Biomedical Engineering, School of Medicine, Oregon Health and Science UniversitySchool of Dentistry, University of BirminghamDepartment of Endodontology, School of Dentistry, Oregon Health and Science UniversityDivision of Biomaterials and Biomechanics, Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science UniversityDivision of Biomaterials and Biomechanics, Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science UniversityAbstract The requirement for immediate vascularization of engineered dental pulp poses a major hurdle towards successful implementation of pulp regeneration as an effective therapeutic strategy for root canal therapy, especially in adult teeth. Here, we demonstrate a novel strategy to engineer pre-vascularized, cell-laden hydrogel pulp-like tissue constructs in full-length root canals for dental pulp regeneration. We utilized gelatin methacryloyl (GelMA) hydrogels with tunable physical and mechanical properties to determine the microenvironmental conditions (microstructure, degradation, swelling and elastic modulus) that enhanced viability, spreading and proliferation of encapsulated odontoblast-like cells (OD21), and the formation of endothelial monolayers by endothelial colony forming cells (ECFCs). GelMA hydrogels with higher polymer concentration (15% w/v) and stiffness enhanced OD21 cell viability, spreading and proliferation, as well as endothelial cell spreading and monolayer formation. We then fabricated pre-vascularized, full-length, dental pulp-like tissue constructs by dispensing OD21 cell-laden GelMA hydrogel prepolymer in root canals of extracted teeth and fabricating 500 µm channels throughout the root canals. ECFCs seeded into the microchannels successfully formed monolayers and underwent angiogenic sprouting within 7 days in culture. In summary, the proposed approach is a simple and effective strategy for engineering of pre-vascularized dental pulp constructs offering potentially beneficial translational outcomes.https://doi.org/10.1038/s41598-017-02532-3 |
| spellingShingle | Avathamsa Athirasala Fernanda Lins Anthony Tahayeri Monica Hinds Anthony J. Smith Christine Sedgley Jack Ferracane Luiz E. Bertassoni A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs Scientific Reports |
| title | A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs |
| title_full | A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs |
| title_fullStr | A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs |
| title_full_unstemmed | A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs |
| title_short | A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs |
| title_sort | novel strategy to engineer pre vascularized full length dental pulp like tissue constructs |
| url | https://doi.org/10.1038/s41598-017-02532-3 |
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