A Modified d-ROMs Method for Determination of Lipid Peroxidation
The need for simple and low costing and less time consuming methods for the determination of lipid peroxides and oxidative stress is urgent because oxidative stress is still unable to become a routine laboratory biomarker due to the sophisticated procedures and costing, a matter that made oxidative...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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"Vikol publishing" ST Kolesnichenko V.V.
2022-06-01
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| Series: | Journal of Stress Physiology & Biochemistry |
| Subjects: | |
| Online Access: | http://www.jspb.ru/issues/2022/N2/JSPB_2022_2_93-95.pdf |
| _version_ | 1811250681797935104 |
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| author | Muammar A Y Nassar Saleh H Sharif Khalda M A Hammad |
| author_facet | Muammar A Y Nassar Saleh H Sharif Khalda M A Hammad |
| author_sort | Muammar A Y Nassar |
| collection | DOAJ |
| description | The need for simple and low costing and less time consuming methods for the determination of lipid peroxides and oxidative stress is urgent because oxidative stress is still unable to become a routine laboratory biomarker due to the sophisticated procedures and costing, a matter that made oxidative stress a research only biomarker, despite its crucial value as a risk factor and pathologic status, for many inflammatory and metabolic diseases. This work aimed at developing a modified rapid method to determine serum lipid peroxides at wavelength 492 nm and reference wavelength 630 nm. The results showed linear responses with increasing concentration and that the cheap N,N-DM-P-Phenylenediamine diHCl can be used as a chromogen under saving conditions of pH, temperatures and volume of sample. |
| first_indexed | 2024-04-12T16:08:19Z |
| format | Article |
| id | doaj.art-ac569da837674f71a96eae3c6c2d0485 |
| institution | Directory Open Access Journal |
| issn | 1997-0838 |
| language | English |
| last_indexed | 2024-04-12T16:08:19Z |
| publishDate | 2022-06-01 |
| publisher | "Vikol publishing" ST Kolesnichenko V.V. |
| record_format | Article |
| series | Journal of Stress Physiology & Biochemistry |
| spelling | doaj.art-ac569da837674f71a96eae3c6c2d04852022-12-22T03:25:59Zeng"Vikol publishing" ST Kolesnichenko V.V.Journal of Stress Physiology & Biochemistry1997-08382022-06-011829395A Modified d-ROMs Method for Determination of Lipid PeroxidationMuammar A Y Nassar0Saleh H Sharif1Khalda M A Hammad2The Biomedical Research Laboratory (BRL) group, Faculty of Medicine and Health Sciences, University of Dongola, Dongola, SudanThe Biomedical Research Laboratory (BRL) group, Faculty of Medicine and Health Sciences, University of Dongola, Dongola, SudanThe Biomedical Research Laboratory (BRL) group, Faculty of Medicine and Health Sciences, University of Dongola, Dongola, SudanThe need for simple and low costing and less time consuming methods for the determination of lipid peroxides and oxidative stress is urgent because oxidative stress is still unable to become a routine laboratory biomarker due to the sophisticated procedures and costing, a matter that made oxidative stress a research only biomarker, despite its crucial value as a risk factor and pathologic status, for many inflammatory and metabolic diseases. This work aimed at developing a modified rapid method to determine serum lipid peroxides at wavelength 492 nm and reference wavelength 630 nm. The results showed linear responses with increasing concentration and that the cheap N,N-DM-P-Phenylenediamine diHCl can be used as a chromogen under saving conditions of pH, temperatures and volume of sample.http://www.jspb.ru/issues/2022/N2/JSPB_2022_2_93-95.pdfdetermination of lipid peroxidesmodified rapid method |
| spellingShingle | Muammar A Y Nassar Saleh H Sharif Khalda M A Hammad A Modified d-ROMs Method for Determination of Lipid Peroxidation Journal of Stress Physiology & Biochemistry determination of lipid peroxides modified rapid method |
| title | A Modified d-ROMs Method for Determination of Lipid Peroxidation |
| title_full | A Modified d-ROMs Method for Determination of Lipid Peroxidation |
| title_fullStr | A Modified d-ROMs Method for Determination of Lipid Peroxidation |
| title_full_unstemmed | A Modified d-ROMs Method for Determination of Lipid Peroxidation |
| title_short | A Modified d-ROMs Method for Determination of Lipid Peroxidation |
| title_sort | modified d roms method for determination of lipid peroxidation |
| topic | determination of lipid peroxides modified rapid method |
| url | http://www.jspb.ru/issues/2022/N2/JSPB_2022_2_93-95.pdf |
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