The Study of the Therapeutic Effects of CpG Oligonucleotides in Murine Model of Allergic Asthma
Background and objectivesAugmentation of Th2 mediated responses such as elevation of serum IgE is the characteristic feature in atopy and asthma. CpG oligonucleotides (CpG ODN) have been used during the recent two decades as the potent immunomodulatory agents in allergic conditions. In this paper, w...
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| Format: | Article |
| Language: | fas |
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Qom University of Medical Sciences
2007-07-01
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| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
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| Online Access: | http://journal.muq.ac.ir/library/upload/article/86.2.1.pdf |
| _version_ | 1818179691462262784 |
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| author | T. Mousavi A.R. Salek Moghaddam R. Falak M. Hejazi N. Asadi |
| author_facet | T. Mousavi A.R. Salek Moghaddam R. Falak M. Hejazi N. Asadi |
| author_sort | T. Mousavi |
| collection | DOAJ |
| description | Background and objectivesAugmentation of Th2 mediated responses such as elevation of serum IgE is the characteristic feature in atopy and asthma. CpG oligonucleotides (CpG ODN) have been used during the recent two decades as the potent immunomodulatory agents in allergic conditions. In this paper, we aim to report the anti-inflammatory effects of CPG ODN in murine model of asthma. Methods In this study, BALB/c mice were sensitized with ch.a. allergen and then treated intranasally with CpG ODN. Following the challenge with allergen inhalation, mice were sacrificed and their splenocytes were cultured in the presence of antigen. After three days of culture, supernatants were examined for IFN-γ levels by ELISA method, as an indication of Th1 response, and the results were compared with those in control mice without CpG therapy. Results The results indicate that the mice in the test group, which received CpG ODN, showed higher levels of systemic INF-γ, and lower levels of serum IgE compared with either the antigen or CpG negative ODN-treated groups.Conclusion Based on the results of this study, which shows higher INF-γ levels in mice received CpG ODN, it can be concluded that CpG motifs have immune response modulation potential apparently through the deviation of Th2 into Th1 responses, which lead to a decrease in IgE antibody level. Accordingly, we suggest that these components can have therapeutic effects in the asthma caused by ch.a. allergen.Keywords: Asthma, CpG oligonucleotides, Chenopodium Album |
| first_indexed | 2024-12-11T21:07:54Z |
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| id | doaj.art-ac571c15507545d7a9ef9c2016c86703 |
| institution | Directory Open Access Journal |
| issn | 1735-7799 2008-1375 |
| language | fas |
| last_indexed | 2024-12-11T21:07:54Z |
| publishDate | 2007-07-01 |
| publisher | Qom University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| spelling | doaj.art-ac571c15507545d7a9ef9c2016c867032022-12-22T00:50:48ZfasQom University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum1735-77992008-13752007-07-0112714The Study of the Therapeutic Effects of CpG Oligonucleotides in Murine Model of Allergic AsthmaT. MousaviA.R. Salek MoghaddamR. FalakM. HejaziN. AsadiBackground and objectivesAugmentation of Th2 mediated responses such as elevation of serum IgE is the characteristic feature in atopy and asthma. CpG oligonucleotides (CpG ODN) have been used during the recent two decades as the potent immunomodulatory agents in allergic conditions. In this paper, we aim to report the anti-inflammatory effects of CPG ODN in murine model of asthma. Methods In this study, BALB/c mice were sensitized with ch.a. allergen and then treated intranasally with CpG ODN. Following the challenge with allergen inhalation, mice were sacrificed and their splenocytes were cultured in the presence of antigen. After three days of culture, supernatants were examined for IFN-γ levels by ELISA method, as an indication of Th1 response, and the results were compared with those in control mice without CpG therapy. Results The results indicate that the mice in the test group, which received CpG ODN, showed higher levels of systemic INF-γ, and lower levels of serum IgE compared with either the antigen or CpG negative ODN-treated groups.Conclusion Based on the results of this study, which shows higher INF-γ levels in mice received CpG ODN, it can be concluded that CpG motifs have immune response modulation potential apparently through the deviation of Th2 into Th1 responses, which lead to a decrease in IgE antibody level. Accordingly, we suggest that these components can have therapeutic effects in the asthma caused by ch.a. allergen.Keywords: Asthma, CpG oligonucleotides, Chenopodium Albumhttp://journal.muq.ac.ir/library/upload/article/86.2.1.pdfAsthmaCpG oligonucleotidesChenopodium Album |
| spellingShingle | T. Mousavi A.R. Salek Moghaddam R. Falak M. Hejazi N. Asadi The Study of the Therapeutic Effects of CpG Oligonucleotides in Murine Model of Allergic Asthma Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum Asthma CpG oligonucleotides Chenopodium Album |
| title | The Study of the Therapeutic Effects of CpG Oligonucleotides in Murine Model of Allergic Asthma |
| title_full | The Study of the Therapeutic Effects of CpG Oligonucleotides in Murine Model of Allergic Asthma |
| title_fullStr | The Study of the Therapeutic Effects of CpG Oligonucleotides in Murine Model of Allergic Asthma |
| title_full_unstemmed | The Study of the Therapeutic Effects of CpG Oligonucleotides in Murine Model of Allergic Asthma |
| title_short | The Study of the Therapeutic Effects of CpG Oligonucleotides in Murine Model of Allergic Asthma |
| title_sort | study of the therapeutic effects of cpg oligonucleotides in murine model of allergic asthma |
| topic | Asthma CpG oligonucleotides Chenopodium Album |
| url | http://journal.muq.ac.ir/library/upload/article/86.2.1.pdf |
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