Metagenomic identification of novel viruses of maize and teosinte in North America
Abstract Background Maize-infecting viruses are known to inflict significant agronomic yield loss throughout the world annually. Identification of known or novel causal agents of disease prior to outbreak is imperative to preserve food security via future crop protection efforts. Toward this goal, a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-022-09001-w |
| _version_ | 1811304618278256640 |
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| author | Ryan R. Lappe Manjula G. Elmore Zachary R. Lozier Georg Jander W. Allen Miller Steven A. Whitham |
| author_facet | Ryan R. Lappe Manjula G. Elmore Zachary R. Lozier Georg Jander W. Allen Miller Steven A. Whitham |
| author_sort | Ryan R. Lappe |
| collection | DOAJ |
| description | Abstract Background Maize-infecting viruses are known to inflict significant agronomic yield loss throughout the world annually. Identification of known or novel causal agents of disease prior to outbreak is imperative to preserve food security via future crop protection efforts. Toward this goal, a large-scale metagenomic approach utilizing high throughput sequencing (HTS) was employed to identify novel viruses with the potential to contribute to yield loss of graminaceous species, particularly maize, in North America. Results Here we present four novel viruses discovered by HTS and individually validated by Sanger sequencing. Three of these viruses are RNA viruses belonging to either the Betaflexiviridae or Tombusviridae families. Additionally, a novel DNA virus belonging to the Geminiviridae family was discovered, the first Mastrevirus identified in North American maize. Conclusions Metagenomic studies of crop and crop-related species such as this may be useful for the identification and surveillance of known and novel viral pathogens of crops. Monitoring related species may prove useful in identifying viruses capable of infecting crops due to overlapping insect vectors and viral host-range to protect food security. |
| first_indexed | 2024-04-13T08:10:39Z |
| format | Article |
| id | doaj.art-ac5c1bc6a5c04753b910b8b57f07dec5 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T08:10:39Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-ac5c1bc6a5c04753b910b8b57f07dec52022-12-22T02:55:01ZengBMCBMC Genomics1471-21642022-11-0123111510.1186/s12864-022-09001-wMetagenomic identification of novel viruses of maize and teosinte in North AmericaRyan R. Lappe0Manjula G. Elmore1Zachary R. Lozier2Georg Jander3W. Allen Miller4Steven A. Whitham5Department of Plant Pathology, Entomology and Microbiology, Iowa State UniversityDepartment of Plant Pathology, Entomology and Microbiology, Iowa State UniversityDepartment of Plant Pathology, Entomology and Microbiology, Iowa State UniversityBoyce Thompson InstituteDepartment of Plant Pathology, Entomology and Microbiology, Iowa State UniversityDepartment of Plant Pathology, Entomology and Microbiology, Iowa State UniversityAbstract Background Maize-infecting viruses are known to inflict significant agronomic yield loss throughout the world annually. Identification of known or novel causal agents of disease prior to outbreak is imperative to preserve food security via future crop protection efforts. Toward this goal, a large-scale metagenomic approach utilizing high throughput sequencing (HTS) was employed to identify novel viruses with the potential to contribute to yield loss of graminaceous species, particularly maize, in North America. Results Here we present four novel viruses discovered by HTS and individually validated by Sanger sequencing. Three of these viruses are RNA viruses belonging to either the Betaflexiviridae or Tombusviridae families. Additionally, a novel DNA virus belonging to the Geminiviridae family was discovered, the first Mastrevirus identified in North American maize. Conclusions Metagenomic studies of crop and crop-related species such as this may be useful for the identification and surveillance of known and novel viral pathogens of crops. Monitoring related species may prove useful in identifying viruses capable of infecting crops due to overlapping insect vectors and viral host-range to protect food security.https://doi.org/10.1186/s12864-022-09001-wMastrevirusTombusvirusUmbravirusBetaflexivirusMaize chlorotic mottle virus |
| spellingShingle | Ryan R. Lappe Manjula G. Elmore Zachary R. Lozier Georg Jander W. Allen Miller Steven A. Whitham Metagenomic identification of novel viruses of maize and teosinte in North America BMC Genomics Mastrevirus Tombusvirus Umbravirus Betaflexivirus Maize chlorotic mottle virus |
| title | Metagenomic identification of novel viruses of maize and teosinte in North America |
| title_full | Metagenomic identification of novel viruses of maize and teosinte in North America |
| title_fullStr | Metagenomic identification of novel viruses of maize and teosinte in North America |
| title_full_unstemmed | Metagenomic identification of novel viruses of maize and teosinte in North America |
| title_short | Metagenomic identification of novel viruses of maize and teosinte in North America |
| title_sort | metagenomic identification of novel viruses of maize and teosinte in north america |
| topic | Mastrevirus Tombusvirus Umbravirus Betaflexivirus Maize chlorotic mottle virus |
| url | https://doi.org/10.1186/s12864-022-09001-w |
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