Human skeletal muscle plasmalemma alters its structure to change its Ca2+-handling following heavy-load resistance exercise
Abstract High-force eccentric exercise results in sustained increases in cytoplasmic Ca2+ levels ([Ca2+]cyto), which can cause damage to the muscle. Here we report that a heavy-load strength training bout greatly alters the structure of the membrane network inside the fibres, the tubular (t-) system...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms14266 |
| _version_ | 1797789652405452800 |
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| author | Tanya R. Cully Robyn M. Murphy Llion Roberts Truls Raastad Robert G. Fassett Jeff S. Coombes Izzy Jayasinghe Bradley S. Launikonis |
| author_facet | Tanya R. Cully Robyn M. Murphy Llion Roberts Truls Raastad Robert G. Fassett Jeff S. Coombes Izzy Jayasinghe Bradley S. Launikonis |
| author_sort | Tanya R. Cully |
| collection | DOAJ |
| description | Abstract High-force eccentric exercise results in sustained increases in cytoplasmic Ca2+ levels ([Ca2+]cyto), which can cause damage to the muscle. Here we report that a heavy-load strength training bout greatly alters the structure of the membrane network inside the fibres, the tubular (t-) system, causing the loss of its predominantly transverse organization and an increase in vacuolation of its longitudinal tubules across adjacent sarcomeres. The transverse tubules and vacuoles displayed distinct Ca2+-handling properties. Both t-system components could take up Ca2+ from the cytoplasm but only transverse tubules supported store-operated Ca2+ entry. The retention of significant amounts of Ca2+ within vacuoles provides an effective mechanism to reduce the total content of Ca2+ within the fibre cytoplasm. We propose this ability can reduce or limit resistance exercise-induced, Ca2+-dependent damage to the fibre by the reduction of [Ca2+]cyto to help maintain fibre viability during the period associated with delayed onset muscle soreness. |
| first_indexed | 2024-03-13T01:53:43Z |
| format | Article |
| id | doaj.art-ac5dc5c88c904369b9a94eb7648f2c36 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-13T01:53:43Z |
| publishDate | 2017-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-ac5dc5c88c904369b9a94eb7648f2c362023-07-02T11:20:49ZengNature PortfolioNature Communications2041-17232017-02-018111010.1038/ncomms14266Human skeletal muscle plasmalemma alters its structure to change its Ca2+-handling following heavy-load resistance exerciseTanya R. Cully0Robyn M. Murphy1Llion Roberts2Truls Raastad3Robert G. Fassett4Jeff S. Coombes5Izzy Jayasinghe6Bradley S. Launikonis7School of Biomedical Sciences, The University of QueenslandDepartment of Biochemistry & Genetics, La Trobe Institute for Molecular Science, La Trobe UniversitySchool of Human Movement and Nutritional Sciences, The University of QueenslandNorwegian School of Sport SciencesSchool of Human Movement and Nutritional Sciences, The University of QueenslandSchool of Human Movement and Nutritional Sciences, The University of QueenslandSchool of Biomedical Sciences, The University of QueenslandSchool of Biomedical Sciences, The University of QueenslandAbstract High-force eccentric exercise results in sustained increases in cytoplasmic Ca2+ levels ([Ca2+]cyto), which can cause damage to the muscle. Here we report that a heavy-load strength training bout greatly alters the structure of the membrane network inside the fibres, the tubular (t-) system, causing the loss of its predominantly transverse organization and an increase in vacuolation of its longitudinal tubules across adjacent sarcomeres. The transverse tubules and vacuoles displayed distinct Ca2+-handling properties. Both t-system components could take up Ca2+ from the cytoplasm but only transverse tubules supported store-operated Ca2+ entry. The retention of significant amounts of Ca2+ within vacuoles provides an effective mechanism to reduce the total content of Ca2+ within the fibre cytoplasm. We propose this ability can reduce or limit resistance exercise-induced, Ca2+-dependent damage to the fibre by the reduction of [Ca2+]cyto to help maintain fibre viability during the period associated with delayed onset muscle soreness.https://doi.org/10.1038/ncomms14266 |
| spellingShingle | Tanya R. Cully Robyn M. Murphy Llion Roberts Truls Raastad Robert G. Fassett Jeff S. Coombes Izzy Jayasinghe Bradley S. Launikonis Human skeletal muscle plasmalemma alters its structure to change its Ca2+-handling following heavy-load resistance exercise Nature Communications |
| title | Human skeletal muscle plasmalemma alters its structure to change its Ca2+-handling following heavy-load resistance exercise |
| title_full | Human skeletal muscle plasmalemma alters its structure to change its Ca2+-handling following heavy-load resistance exercise |
| title_fullStr | Human skeletal muscle plasmalemma alters its structure to change its Ca2+-handling following heavy-load resistance exercise |
| title_full_unstemmed | Human skeletal muscle plasmalemma alters its structure to change its Ca2+-handling following heavy-load resistance exercise |
| title_short | Human skeletal muscle plasmalemma alters its structure to change its Ca2+-handling following heavy-load resistance exercise |
| title_sort | human skeletal muscle plasmalemma alters its structure to change its ca2 handling following heavy load resistance exercise |
| url | https://doi.org/10.1038/ncomms14266 |
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