Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl
<i>Centaurea cyanus</i>, belonging to the Asteraceae family, is an arable weed species encountered mainly in fields with cereals, sugar beet, and maize. The high genetic variability of <i>C. cyanus</i> has been recently reported; however, little is known about its sequence va...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/11/2311 |
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| author | Barbara Wrzesińska Tadeusz Praczyk |
| author_facet | Barbara Wrzesińska Tadeusz Praczyk |
| author_sort | Barbara Wrzesińska |
| collection | DOAJ |
| description | <i>Centaurea cyanus</i>, belonging to the Asteraceae family, is an arable weed species encountered mainly in fields with cereals, sugar beet, and maize. The high genetic variability of <i>C. cyanus</i> has been recently reported; however, little is known about its sequence variability in the context of its herbicide resistance. <i>C. cyanus</i> resistance was found mainly against acetolactate synthase (ALS) inhibitors, but no ALS sequence information concerning the herbicide resistance mechanism has been published yet. The aim of this study was to determine the <i>ALS</i> sequences for biotypes susceptible and resistant to tribenuron-methyl in order to identify mutations that may be associated with the resistance emergence. DNA isolation from susceptible and resistant plants was followed by PCR amplification and <i>ALS</i> sequencing. As a result, different lengths of DNA products were obtained. Moreover, both nucleotide and amino acid sequence analysis revealed high sequence variability within one plant as well as between plants from the same biotype. In a few resistant plants, four changes in the amino acid sequence were identified in comparison to those in the susceptible ones. However, these preliminary studies require further investigation toward confirming the significance of these mutations in herbicide resistance development. This study provides preliminary information contributing to the research on the <i>C. cyanus</i> target-site resistance mechanism. |
| first_indexed | 2024-03-10T05:47:21Z |
| format | Article |
| id | doaj.art-ac5e9d0912924af080303e9d5db7ca9e |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T05:47:21Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-ac5e9d0912924af080303e9d5db7ca9e2023-11-22T22:03:41ZengMDPI AGAgronomy2073-43952021-11-011111231110.3390/agronomy11112311Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-MethylBarbara Wrzesińska0Tadeusz Praczyk1Department of Molecular Biology and Biotechnology, Institute of Plant Protection—National Research Institute, 60-318 Poznań, PolandDepartment of Weed Science and Plant Protection Techniques, Institute of Plant Protection—National Research Institute, 60-318 Poznań, Poland<i>Centaurea cyanus</i>, belonging to the Asteraceae family, is an arable weed species encountered mainly in fields with cereals, sugar beet, and maize. The high genetic variability of <i>C. cyanus</i> has been recently reported; however, little is known about its sequence variability in the context of its herbicide resistance. <i>C. cyanus</i> resistance was found mainly against acetolactate synthase (ALS) inhibitors, but no ALS sequence information concerning the herbicide resistance mechanism has been published yet. The aim of this study was to determine the <i>ALS</i> sequences for biotypes susceptible and resistant to tribenuron-methyl in order to identify mutations that may be associated with the resistance emergence. DNA isolation from susceptible and resistant plants was followed by PCR amplification and <i>ALS</i> sequencing. As a result, different lengths of DNA products were obtained. Moreover, both nucleotide and amino acid sequence analysis revealed high sequence variability within one plant as well as between plants from the same biotype. In a few resistant plants, four changes in the amino acid sequence were identified in comparison to those in the susceptible ones. However, these preliminary studies require further investigation toward confirming the significance of these mutations in herbicide resistance development. This study provides preliminary information contributing to the research on the <i>C. cyanus</i> target-site resistance mechanism.https://www.mdpi.com/2073-4395/11/11/2311<i>Centaurea cyanus</i>herbicide resistanceALS sequenceacetolactate synthasecornflowergenetic variability |
| spellingShingle | Barbara Wrzesińska Tadeusz Praczyk Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl Agronomy <i>Centaurea cyanus</i> herbicide resistance ALS sequence acetolactate synthase cornflower genetic variability |
| title | Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl |
| title_full | Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl |
| title_fullStr | Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl |
| title_full_unstemmed | Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl |
| title_short | Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl |
| title_sort | genetic variability of acetolactate synthase als sequence in i centaurea cyanus i plants resistant and susceptible to tribenuron methyl |
| topic | <i>Centaurea cyanus</i> herbicide resistance ALS sequence acetolactate synthase cornflower genetic variability |
| url | https://www.mdpi.com/2073-4395/11/11/2311 |
| work_keys_str_mv | AT barbarawrzesinska geneticvariabilityofacetolactatesynthasealssequenceinicentaureacyanusiplantsresistantandsusceptibletotribenuronmethyl AT tadeuszpraczyk geneticvariabilityofacetolactatesynthasealssequenceinicentaureacyanusiplantsresistantandsusceptibletotribenuronmethyl |