Cross-talk between AMP-activated protein kinase and renin–angiotensin system in uninephrectomised rats
Introduction: The renal renin–angiotensin system (RAS) and the ultrasensitive energy sensor AMP-activated protein kinase (AMPK) have been implicated in normal and aberrant states of the kidney, but interaction between the RAS and AMPK remains unknown. Methods: Ninety-six rats were stratified into fo...
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| Format: | Article |
| Language: | English |
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SAGE Publications
2016-10-01
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| Series: | Journal of the Renin-Angiotensin-Aldosterone System |
| Online Access: | https://doi.org/10.1177/1470320316673231 |
| _version_ | 1797281411847159808 |
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| author | Ke-Ke Yang Yi Sui Hui-Rong Zhou Jian Shen Ning Tan Yan-Mei Huang Sha-Sha LI Yan-Hong Pan Xiao-Xi Zhang Hai-Lu Zhao |
| author_facet | Ke-Ke Yang Yi Sui Hui-Rong Zhou Jian Shen Ning Tan Yan-Mei Huang Sha-Sha LI Yan-Hong Pan Xiao-Xi Zhang Hai-Lu Zhao |
| author_sort | Ke-Ke Yang |
| collection | DOAJ |
| description | Introduction: The renal renin–angiotensin system (RAS) and the ultrasensitive energy sensor AMP-activated protein kinase (AMPK) have been implicated in normal and aberrant states of the kidney, but interaction between the RAS and AMPK remains unknown. Methods: Ninety-six rats were stratified into four groups: sham, uninephrectomised, uninephrectomised rats treated with the angiotensin-converting enzyme inhibitor lisinopril or the angiotensin receptor blocker losartan. Histopathological examination at 9 months post-operation and biochemical measurements at 3, 6 and 9 months were performed for changes in renal structure and function. The expression of AMPK and angiotensin II at 9 months was detected by immunofluorescence microscopy and western blot. Results: Compared with sham rats, uninephrectomised rats demonstrated progressive glomerulosclerosis, tubular atrophy with cast formation and chronic inflammatory infiltration, in parallel to elevated serum urea, creatinine, urine total protein to creatinine ratio and reduced serum albumin. Overexpression of angiotensin II coexisted with a 85.6% reduction of phosphorylated to total AMPK ratio in the remnant kidney of uninephrectomised rats. RAS blockade by the angiotensin-converting enzyme inhibitor or angiotensin receptor blocker substantially normalised AMPK expression, morphological and functional changes of the remnant kidney. Conclusions: Uninephrectomy-induced RAS activation and AMPK inhibition in the remnant kidney could be substantially corrected by RAS blockade, suggesting a cross-talk between AMPK and RAS components in uninephrectomised rats. |
| first_indexed | 2024-03-07T16:57:15Z |
| format | Article |
| id | doaj.art-ac6003e6b86447ac9ec93cf6b3acd9fe |
| institution | Directory Open Access Journal |
| issn | 1752-8976 |
| language | English |
| last_indexed | 2024-03-07T16:57:15Z |
| publishDate | 2016-10-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Journal of the Renin-Angiotensin-Aldosterone System |
| spelling | doaj.art-ac6003e6b86447ac9ec93cf6b3acd9fe2024-03-03T03:42:17ZengSAGE PublicationsJournal of the Renin-Angiotensin-Aldosterone System1752-89762016-10-011710.1177/147032031667323110.1177_1470320316673231Cross-talk between AMP-activated protein kinase and renin–angiotensin system in uninephrectomised ratsKe-Ke Yang0Yi Sui1Hui-Rong Zhou2Jian Shen3Ning Tan4Yan-Mei Huang5Sha-Sha LI6Yan-Hong Pan7Xiao-Xi Zhang8Hai-Lu Zhao9Department of Laboratory, The Second Affiliated Hospital of Nantong University, ChinaCenter for Diabetic Systems Medicine, Guilin Medical University, ChinaDepartment of Surgical Pathology, Shenzhen Hospital of Southern Medical University, ChinaCenter for Diabetic Systems Medicine, Guilin Medical University, ChinaCentral Laboratory of Core Facilities, Guilin Medical University, ChinaCenter for Diabetic Systems Medicine, Guilin Medical University, ChinaCenter for Diabetic Systems Medicine, Guilin Medical University, ChinaCenter for Diabetic Systems Medicine, Guilin Medical University, ChinaCenter for Diabetic Systems Medicine, Guilin Medical University, ChinaCenter for Diabetic Systems Medicine, Guilin Medical University, ChinaIntroduction: The renal renin–angiotensin system (RAS) and the ultrasensitive energy sensor AMP-activated protein kinase (AMPK) have been implicated in normal and aberrant states of the kidney, but interaction between the RAS and AMPK remains unknown. Methods: Ninety-six rats were stratified into four groups: sham, uninephrectomised, uninephrectomised rats treated with the angiotensin-converting enzyme inhibitor lisinopril or the angiotensin receptor blocker losartan. Histopathological examination at 9 months post-operation and biochemical measurements at 3, 6 and 9 months were performed for changes in renal structure and function. The expression of AMPK and angiotensin II at 9 months was detected by immunofluorescence microscopy and western blot. Results: Compared with sham rats, uninephrectomised rats demonstrated progressive glomerulosclerosis, tubular atrophy with cast formation and chronic inflammatory infiltration, in parallel to elevated serum urea, creatinine, urine total protein to creatinine ratio and reduced serum albumin. Overexpression of angiotensin II coexisted with a 85.6% reduction of phosphorylated to total AMPK ratio in the remnant kidney of uninephrectomised rats. RAS blockade by the angiotensin-converting enzyme inhibitor or angiotensin receptor blocker substantially normalised AMPK expression, morphological and functional changes of the remnant kidney. Conclusions: Uninephrectomy-induced RAS activation and AMPK inhibition in the remnant kidney could be substantially corrected by RAS blockade, suggesting a cross-talk between AMPK and RAS components in uninephrectomised rats.https://doi.org/10.1177/1470320316673231 |
| spellingShingle | Ke-Ke Yang Yi Sui Hui-Rong Zhou Jian Shen Ning Tan Yan-Mei Huang Sha-Sha LI Yan-Hong Pan Xiao-Xi Zhang Hai-Lu Zhao Cross-talk between AMP-activated protein kinase and renin–angiotensin system in uninephrectomised rats Journal of the Renin-Angiotensin-Aldosterone System |
| title | Cross-talk between AMP-activated protein kinase and renin–angiotensin system in uninephrectomised rats |
| title_full | Cross-talk between AMP-activated protein kinase and renin–angiotensin system in uninephrectomised rats |
| title_fullStr | Cross-talk between AMP-activated protein kinase and renin–angiotensin system in uninephrectomised rats |
| title_full_unstemmed | Cross-talk between AMP-activated protein kinase and renin–angiotensin system in uninephrectomised rats |
| title_short | Cross-talk between AMP-activated protein kinase and renin–angiotensin system in uninephrectomised rats |
| title_sort | cross talk between amp activated protein kinase and renin angiotensin system in uninephrectomised rats |
| url | https://doi.org/10.1177/1470320316673231 |
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