Hypoxia Activates <i>HIF-1α</i> and Affects Gene Expression and Transcriptional Regulation of <i>PHD</i> in <i>Tegillarca granosa</i>
Hypoxia is a common challenge faced by mollusks, and the role of hypoxia-inducible factor-1α (<i>HIF-1α</i>) in regulating related target genes under hypoxia in <i>Tegillarca granosa (Tg</i>) remains unclear. In this study, we identified gene <i>HIF-1α</i> and fur...
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MDPI AG
2023-07-01
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| Online Access: | https://www.mdpi.com/2410-3888/8/7/359 |
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| author | Zhilan Peng Xiaomin Liu Ming Jin Yu Zhan Xiaolin Zhang Yongbo Bao Minhai Liu |
| author_facet | Zhilan Peng Xiaomin Liu Ming Jin Yu Zhan Xiaolin Zhang Yongbo Bao Minhai Liu |
| author_sort | Zhilan Peng |
| collection | DOAJ |
| description | Hypoxia is a common challenge faced by mollusks, and the role of hypoxia-inducible factor-1α (<i>HIF-1α</i>) in regulating related target genes under hypoxia in <i>Tegillarca granosa (Tg</i>) remains unclear. In this study, we identified gene <i>HIF-1α</i> and further explored its function. qRT-PCR was performed to determine the mRNA expression of <i>HIF-1α</i>, prolyl 4-hydroxylases (<i>PHD</i>), hemoglobin <i>(Hb)</i>, and myoglobin <i>(Mb)</i> in response to hypoxia. Dual-luciferase reporter analysis was used to assess the transcriptional activity of <i>HIF-1α</i> on the <i>PHD</i> and <i>Hb</i> genes during hypoxia. Results showed that the expression levels of <i>HIF-1α</i> and <i>PHD</i> mRNAs were highest in the gill and lowest in the adductor muscle. Under hypoxic conditions, <i>HIF-1α</i> and <i>PHD</i> mRNAs were both induced, and their expression levels increased significantly, peaking at 8 h and gradually decreasing thereafter. The transcription of <i>Tg-PHD</i> was induced by hypoxia and was <i>Tg-HIF-1α</i> dependent. Notably, the expression of <i>Hb</i> decreased in hemocytes (<i>p</i> < 0.01) after 8 h at 0.5 mg/L, while the expression of <i>MbI</i> and <i>MbII</i> increased (<i>p</i> < 0.01) in the hepatopancreas after 24 h and 120 h, respectively. Moreover, <i>Tg-HIF-1α</i> could transactivate the <i>PHD</i> promoter but not that of <i>Hb</i>. These findings provide valuable insights into the regulatory role of <i>Tg-HIF-1α</i> on <i>Tg-PHD</i>, safeguarding it from degradation by <i>PHD</i> and offering significant contributions to the understanding of <i>HIF-1α’s</i> function. Nonetheless, the expression of Tg-HIF-1α protein was not detected under hypoxic conditions, and additional investigations are required to quantify Tg-HIF-1α protein dynamics and establish the correlation between mRNA expression and protein levels. |
| first_indexed | 2024-03-11T01:05:54Z |
| format | Article |
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| last_indexed | 2024-03-11T01:05:54Z |
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| spelling | doaj.art-ac61838878b94e8c994e726bf3501dcf2023-11-18T19:18:15ZengMDPI AGFishes2410-38882023-07-018735910.3390/fishes8070359Hypoxia Activates <i>HIF-1α</i> and Affects Gene Expression and Transcriptional Regulation of <i>PHD</i> in <i>Tegillarca granosa</i>Zhilan Peng0Xiaomin Liu1Ming Jin2Yu Zhan3Xiaolin Zhang4Yongbo Bao5Minhai Liu6Key Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, ChinaKey Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, ChinaKey Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, ChinaSchool of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaLaboratory of Marine Biology Resource and Molecular Engineering, Marine Science and Technical College, Zhejiang Ocean University, Zhoushan 316022, ChinaKey Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, ChinaNinghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ningbo 315100, ChinaHypoxia is a common challenge faced by mollusks, and the role of hypoxia-inducible factor-1α (<i>HIF-1α</i>) in regulating related target genes under hypoxia in <i>Tegillarca granosa (Tg</i>) remains unclear. In this study, we identified gene <i>HIF-1α</i> and further explored its function. qRT-PCR was performed to determine the mRNA expression of <i>HIF-1α</i>, prolyl 4-hydroxylases (<i>PHD</i>), hemoglobin <i>(Hb)</i>, and myoglobin <i>(Mb)</i> in response to hypoxia. Dual-luciferase reporter analysis was used to assess the transcriptional activity of <i>HIF-1α</i> on the <i>PHD</i> and <i>Hb</i> genes during hypoxia. Results showed that the expression levels of <i>HIF-1α</i> and <i>PHD</i> mRNAs were highest in the gill and lowest in the adductor muscle. Under hypoxic conditions, <i>HIF-1α</i> and <i>PHD</i> mRNAs were both induced, and their expression levels increased significantly, peaking at 8 h and gradually decreasing thereafter. The transcription of <i>Tg-PHD</i> was induced by hypoxia and was <i>Tg-HIF-1α</i> dependent. Notably, the expression of <i>Hb</i> decreased in hemocytes (<i>p</i> < 0.01) after 8 h at 0.5 mg/L, while the expression of <i>MbI</i> and <i>MbII</i> increased (<i>p</i> < 0.01) in the hepatopancreas after 24 h and 120 h, respectively. Moreover, <i>Tg-HIF-1α</i> could transactivate the <i>PHD</i> promoter but not that of <i>Hb</i>. These findings provide valuable insights into the regulatory role of <i>Tg-HIF-1α</i> on <i>Tg-PHD</i>, safeguarding it from degradation by <i>PHD</i> and offering significant contributions to the understanding of <i>HIF-1α’s</i> function. Nonetheless, the expression of Tg-HIF-1α protein was not detected under hypoxic conditions, and additional investigations are required to quantify Tg-HIF-1α protein dynamics and establish the correlation between mRNA expression and protein levels.https://www.mdpi.com/2410-3888/8/7/359hypoxia<i>HIF-1α</i><i>PHD</i><i>Tegillarca granosa</i>qRT-PCRtranscriptional regulation |
| spellingShingle | Zhilan Peng Xiaomin Liu Ming Jin Yu Zhan Xiaolin Zhang Yongbo Bao Minhai Liu Hypoxia Activates <i>HIF-1α</i> and Affects Gene Expression and Transcriptional Regulation of <i>PHD</i> in <i>Tegillarca granosa</i> Fishes hypoxia <i>HIF-1α</i> <i>PHD</i> <i>Tegillarca granosa</i> qRT-PCR transcriptional regulation |
| title | Hypoxia Activates <i>HIF-1α</i> and Affects Gene Expression and Transcriptional Regulation of <i>PHD</i> in <i>Tegillarca granosa</i> |
| title_full | Hypoxia Activates <i>HIF-1α</i> and Affects Gene Expression and Transcriptional Regulation of <i>PHD</i> in <i>Tegillarca granosa</i> |
| title_fullStr | Hypoxia Activates <i>HIF-1α</i> and Affects Gene Expression and Transcriptional Regulation of <i>PHD</i> in <i>Tegillarca granosa</i> |
| title_full_unstemmed | Hypoxia Activates <i>HIF-1α</i> and Affects Gene Expression and Transcriptional Regulation of <i>PHD</i> in <i>Tegillarca granosa</i> |
| title_short | Hypoxia Activates <i>HIF-1α</i> and Affects Gene Expression and Transcriptional Regulation of <i>PHD</i> in <i>Tegillarca granosa</i> |
| title_sort | hypoxia activates i hif 1α i and affects gene expression and transcriptional regulation of i phd i in i tegillarca granosa i |
| topic | hypoxia <i>HIF-1α</i> <i>PHD</i> <i>Tegillarca granosa</i> qRT-PCR transcriptional regulation |
| url | https://www.mdpi.com/2410-3888/8/7/359 |
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