Anti-Melanogenic Effects of <i>Paederia foetida</i> L. Extract via MAPK Signaling-Mediated MITF Downregulation
In this study, in order to explore the anti-melanogenic effect of PFE (<i>Paederia foetida</i> L. extract) and suggest its availability, B16F10 cells, which are murine melanoma cells, were stimulated with alpha-Melanocyte-stimulating hormone (α-MSH) to conduct an in vitro experiment. Tre...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Cosmetics |
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| Online Access: | https://www.mdpi.com/2079-9284/8/1/22 |
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| author | You Chul Chung Jung No Lee Bong Seok Kim Chang-Gu Hyun |
| author_facet | You Chul Chung Jung No Lee Bong Seok Kim Chang-Gu Hyun |
| author_sort | You Chul Chung |
| collection | DOAJ |
| description | In this study, in order to explore the anti-melanogenic effect of PFE (<i>Paederia foetida</i> L. extract) and suggest its availability, B16F10 cells, which are murine melanoma cells, were stimulated with alpha-Melanocyte-stimulating hormone (α-MSH) to conduct an in vitro experiment. Treatment with PFE in B16F10 cells with activated melanogenesis due to stimulants showed that PFE significantly inhibits melanin content as well as intracellular tyrosinase activity within a range that does not cause cytotoxicity. In addition, Western blot assay demonstrated that PFE strongly inhibited the protein expression of not only tyrosinase-related protein (TRP)-1, -2, and tyrosinase, but also microphthalmia-associated transcription factor (MITF). Moreover, mechanism studies have shown that PFE processing inhibited the activation of melanin production by regulating the phosphorylation of each mitogen-activated protein kinase (MAPK) family in the MAPK signaling pathway. To test the biocompatibility of PFE on human skin, a primary skin irritation test was performed. The results revealed that PFE did not have any side effects on human skin. These findings suggest that PFE holds great potential as a skin whitening agent and in the prevention of hyperpigmentation disorders. |
| first_indexed | 2024-03-10T13:14:47Z |
| format | Article |
| id | doaj.art-ac62e48d8820463d95163717b730425f |
| institution | Directory Open Access Journal |
| issn | 2079-9284 |
| language | English |
| last_indexed | 2024-03-10T13:14:47Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cosmetics |
| spelling | doaj.art-ac62e48d8820463d95163717b730425f2023-11-21T10:30:01ZengMDPI AGCosmetics2079-92842021-03-01812210.3390/cosmetics8010022Anti-Melanogenic Effects of <i>Paederia foetida</i> L. Extract via MAPK Signaling-Mediated MITF DownregulationYou Chul Chung0Jung No Lee1Bong Seok Kim2Chang-Gu Hyun3Jeju Inside Agency & Cosmetic Science Center, Department of Chemistry and Cosmetics, Jeju National University, Jeju 63243, KoreaCoseedBioPharm Co., Ltd., Jeju 63309, KoreaBio-Convergence Center, Jeju Technopark, Jeju 63243, KoreaJeju Inside Agency & Cosmetic Science Center, Department of Chemistry and Cosmetics, Jeju National University, Jeju 63243, KoreaIn this study, in order to explore the anti-melanogenic effect of PFE (<i>Paederia foetida</i> L. extract) and suggest its availability, B16F10 cells, which are murine melanoma cells, were stimulated with alpha-Melanocyte-stimulating hormone (α-MSH) to conduct an in vitro experiment. Treatment with PFE in B16F10 cells with activated melanogenesis due to stimulants showed that PFE significantly inhibits melanin content as well as intracellular tyrosinase activity within a range that does not cause cytotoxicity. In addition, Western blot assay demonstrated that PFE strongly inhibited the protein expression of not only tyrosinase-related protein (TRP)-1, -2, and tyrosinase, but also microphthalmia-associated transcription factor (MITF). Moreover, mechanism studies have shown that PFE processing inhibited the activation of melanin production by regulating the phosphorylation of each mitogen-activated protein kinase (MAPK) family in the MAPK signaling pathway. To test the biocompatibility of PFE on human skin, a primary skin irritation test was performed. The results revealed that PFE did not have any side effects on human skin. These findings suggest that PFE holds great potential as a skin whitening agent and in the prevention of hyperpigmentation disorders.https://www.mdpi.com/2079-9284/8/1/22<i>Paederia foetida</i> L.anti-melanogenesisMAPKskin whiteningB16F10 melanoma cellbioactivity |
| spellingShingle | You Chul Chung Jung No Lee Bong Seok Kim Chang-Gu Hyun Anti-Melanogenic Effects of <i>Paederia foetida</i> L. Extract via MAPK Signaling-Mediated MITF Downregulation Cosmetics <i>Paederia foetida</i> L. anti-melanogenesis MAPK skin whitening B16F10 melanoma cell bioactivity |
| title | Anti-Melanogenic Effects of <i>Paederia foetida</i> L. Extract via MAPK Signaling-Mediated MITF Downregulation |
| title_full | Anti-Melanogenic Effects of <i>Paederia foetida</i> L. Extract via MAPK Signaling-Mediated MITF Downregulation |
| title_fullStr | Anti-Melanogenic Effects of <i>Paederia foetida</i> L. Extract via MAPK Signaling-Mediated MITF Downregulation |
| title_full_unstemmed | Anti-Melanogenic Effects of <i>Paederia foetida</i> L. Extract via MAPK Signaling-Mediated MITF Downregulation |
| title_short | Anti-Melanogenic Effects of <i>Paederia foetida</i> L. Extract via MAPK Signaling-Mediated MITF Downregulation |
| title_sort | anti melanogenic effects of i paederia foetida i l extract via mapk signaling mediated mitf downregulation |
| topic | <i>Paederia foetida</i> L. anti-melanogenesis MAPK skin whitening B16F10 melanoma cell bioactivity |
| url | https://www.mdpi.com/2079-9284/8/1/22 |
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